Scientia Silvae Sinicae ›› 2020, Vol. 56 ›› Issue (12): 166-176.doi: 10.11707/j.1001-7488.20201219
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Fei Ren,Jiaqi Zhang,Hengkang Hu,Bi Liang,Youjun Huang,Heqiang Lou,Qixiang Zhang*
Received:
2019-12-03
Online:
2020-12-25
Published:
2021-01-22
Contact:
Qixiang Zhang
CLC Number:
Fei Ren,Jiaqi Zhang,Hengkang Hu,Bi Liang,Youjun Huang,Heqiang Lou,Qixiang Zhang. Expression Stability of Red Fluorescent Protein Gene DsRED in the Regeneration of Walnut (Juglans regia) Plant[J]. Scientia Silvae Sinicae, 2020, 56(12): 166-176.
Fig.1
Red fluorescent protein gene DsRED expressed stably in walnut somatic embryos A. Walnut somatic embryo proliferation 1, 2: DsRED somatic embryos; 3, 4: Control somatic embryos. B. Walnut somatic embryo developmental stages(Bar: 500 μm) 1-4: Globular embryo, heart-shaped embryo, torpedo embryo, cotyledonary embryo of DsRED somatic embryos in bright field; 5-8: Globular embryo, heart-shaped embryo, torpedo embryo, cotyledonary embryo of DsRED somatic embryos in fluorescent field; 9-12: Globular embryo, heart-shaped embryo, torpedo embryo, cotyledonary embryo of control somatic embryos in bright field; 13-16: Globular embryo, heart-shaped embryo, torpedo embryo, cotyledonary embryo of control somatic embryos in fluorescent field."
Fig.2
PCR and qRT-PCR assay of DsRED gene in walnut somatic embryos and tissue-cultured plantlets A. PCR assay of DsRED gene M: Marker; 1: Control somatic embryo; 2: DsRED somatic embryo; 3-5: Root, stem, leaflet of control tissue-cultured plantlet; 6-8: Root, stem, leaflet of DsRED tissue-cultured plantlet. B. Relative expression of DsRED mRNA(P < 0.05)."
Fig.4
Red fluorescent protein gene DsRED expressed stably in walnut tissue-cultured plantlets A. In vitro plantlets 1: DsRED tissue-cultured plantlet; 2: Control tissue-cultured plantlet. B. Appearance of tissue-cultured plantlets(Bar: 3 mm) 1-3: Root, stem, leaflets of DsRED tissue-cultured plantlet in bright field; 4-6: Root, stem, leaflets of DsRED tissue-cultured plantlet in fluorescent field; 7-9: Root, stem, leaflets of control tissue-cultured plantlet in bright field; 10-12: Root, stem, leaflets of control tissue-cultured plantlet in fluorescent field. C. Section(Bar: 600 μm) 1-3: Cross section of root, stem, leaflet of DsRED tissue-cultured plantlet in bright field; 4-6: Cross section of root, stem, leaflets of DsRED tissue-cultured plantlet in fluorescent field; 7-9: Cross section of root, stem, leaflets of control tissue-cultured plantlet in bright field; 10-12: Cross section of root, stem, leaflets of control tissue-cultured plantlet in fluorescent field."
Table 1
Effect of DsRED gene expression on stem and leaf growth of walnut tissue-cultured plantlets"
指标 Index | DsRED (mean±SD) | 对照Control (mean±SD) |
株高Plant height/cm | 5.72±0.53 a | 5.69±0.68 a |
株幅Plant width/cm | 3.54±0.64 a | 3.43±0.72 a |
茎粗Stem diameter/cm | 0.24±0.06 a | 0.26±0.05 a |
复叶数Number of compound leaf | 7±2 a | 7±2 a |
小叶长Leaflet length/cm | 0.97±0.41 a | 0.98±0.38 a |
小叶宽Leaflet width/cm | 0.48±0.35 a | 0.46±0.37 a |
叶面积Leaflet area/cm2 | 0.69±0.42 a | 0.74±0.38 a |
Fig.5
DsRED red fluorescent protein expressed stably in 3-year-old walnut regenerated plants A. 3-year-old regenerated plants 1: DsRED 3-year-old regenerated plant; 2: Control 3-year-old regenerated plant. B. Appearance of 3-year-old regenerated plants(Bar 3, 6, 9, 12: 10 μm; the rest: 1 cm)1-3: Root, leaflet, leaf epidermis of DsRED 3-year-old regenerated plant in bright field; 4-6: Root, leaflet, leaf epidermis of DsRED 3-year-old regenerated plant in fluorescent field; 7-9: Root, leaflet, leaf epidermis of control 3-year-old regenerated plant in bright field; 10-12: Root, leaflet, leaf epidermis of control 3-year-old regenerated plant in fluorescent field. C. Section(Bar: 300 μm) 1, 2: Cross section of root and leaflet of DsRED 3-year-old regenerated plant in bright field; 3, 4: Cross section of root and leaflet of DsRED 3-year-old regenerated plant in fluorescent field; 5, 6: Cross section of root and leaflet of control 3-year-old regenerated plant in bright field; 7, 8: Cross section of root and leaflet of control 3-year-old regenerated plant in fluorescent field."
Table 2
Effect of DsRED gene expression on stem and leaf growth of 3-year-old walnut regenerated plants"
指标Index | DsRED (mean±SD) | 对照Control (mean±SD) |
株高Plant height/cm | 92.88±5.46 a | 94.14±4.37 a |
株幅Plant width/cm | 71.78±4.32 a | 69.24±3.75 a |
茎粗Stem diameter/cm | 0.89±0.18 a | 0.88±0.19 a |
复叶数Number of compound leaf | 11±2 a | 11±2 a |
小叶长Leaflet length/cm | 15.32±3.43 a | 14.52±4.53 a |
小叶宽Leaflet width/cm | 5.84±1.23 a | 5.63±1.55 a |
叶面积Leaflet area/cm2 | 57.41±28.72 a | 50.33±32.19 a |
陈茂功, 韩志群, 林小虎, 等. 转DsRed荧光蛋白的新月弯孢Curvularia lunata菌株构建. 植物保护, 2012, 38 (6): 16- 21.
doi: 10.3969/j.issn.0529-1542.2012.06.004 |
|
Chen M G , Han Z Q , Lin X H , et al. Construction of DsRed-labeling Curvularia lunata. Plant Protection, 2012, 38 (6): 16- 21.
doi: 10.3969/j.issn.0529-1542.2012.06.004 |
|
陈盼飞, 任亚超, 张军, 等. 8年生嫁接转基因杨树Bt毒蛋白的表达与运输. 林业科学, 2016, 52 (7): 46- 52. | |
Chen P F , Ren Y C , Zhang J , et al. Expression and transportation of Bt toxic protein in 8-year-old grafted transgenic poplar. Scientia Silvae Sinicae, 2016, 52 (7): 46- 52. | |
陈盼飞, 左力辉, 王桂英, 等. 盐胁迫下转复合多基因欧美杨107杨幼苗生长及生理响应. 林业科学, 2017, 53 (7): 45- 53. | |
Chen P F , Zuo L H , Wang G Y , et al. Growth and physiological responses of transgenic Populus×euramericana cv. '4/76' with multiple genes under salt stress. Scientia Silvae Sinicae, 2017, 53 (7): 45- 53. | |
高嵩, 何欢, 吕庆雪, 等. 红色荧光蛋白基因DsRED2植物表达载体的构建及遗传转化. 分子植物育种, 2017, 15 (5): 132- 137. | |
Gao S , He H , Lü Q X , et al. Construction and genetic transformation of DsRED2 plant expression vector in red fluorescent protein gene. Molecular Plant Breeding, 2017, 15 (5): 132- 137. | |
李登峰, 杨彩霞, 刘刚. 光声成像报告基因研究进展. 功能与分子医学影像学:电子版, 2018, 7 (4): 41- 45. | |
Li D F , Yang C X , Liu G . Progress in research on photoacoustic imaging reporter genes. Functional and Molecular Medical Imaging:Electronic Edition, 2018, 7 (4): 41- 45. | |
李季, 黄天带, 蔡海滨, 等. 橡胶树转基因植株遗传稳定性分析. 热带作物学报, 2013, 34 (4): 591- 595.
doi: 10.3969/j.issn.1000-2561.2013.04.001 |
|
Li J , Huang T D , Cai H B , et al. Genetic stability analyses in transformants of Hevea brasiliensis. Chinese Journal of Tropical Crops, 2013, 34 (4): 591- 595.
doi: 10.3969/j.issn.1000-2561.2013.04.001 |
|
李卫东, 王冬梅, 黎小瑛, 等. 用细胞学方法研究番木瓜组培苗的遗传稳定性. 云南植物研究, 2006, 28 (6): 645- 648.
doi: 10.3969/j.issn.2095-0845.2006.06.015 |
|
Li W D , Wang D M , Li X Y , et al. Study on the genetic stability of Carica papaya (Caricaceae) propagating plantlets through cytological approach. Acta Botanica Yunnanica, 2006, 28 (6): 645- 648.
doi: 10.3969/j.issn.2095-0845.2006.06.015 |
|
李小方, 汤章城, 何玉科. 不定根的形态发生与调节机制. 细胞生物学杂志, 2001, 23 (3): 130- 136. | |
Li X F , Tang Z C , He Y K . Studies on stability of exogenous gene in transgenic apples. Molecular Plant Breeding, 2001, 23 (3): 130- 136. | |
李新锋, 赵淑清. 转基因植物中报道基因GUS的活性检测及其应用. 生命的化学, 2016, 24 (1): 73- 76. | |
Li X F , Zhao S Q . Assays for GUS activity in transgenic plants and their application in transgenic plants. Chemistry of Life, 2016, 24 (1): 73- 76. | |
刘娜, 万瑛, 周镜然, 等. 红色荧光蛋白与卵白蛋白表位融合蛋白的表达与纯化. 免疫学杂志, 2005, 21 (5): 382- 385.
doi: 10.3969/j.issn.1000-8861.2005.05.009 |
|
Liu N , Wan Y , Zhou J R , et al. Expression and purification of DsRed tagged OVA epitope fusion protein. Immunological Journal, 2005, 21 (5): 382- 385.
doi: 10.3969/j.issn.1000-8861.2005.05.009 |
|
牛东东, 郝育杰, 荣瑞娟, 等. 转基因水稻中GUS蛋白质的检测及其表达特征. 中国农业科学, 2014, 47 (14): 2715- 2722.
doi: 10.3864/j.issn.0578-1752.2014.14.002 |
|
Niu D D , Hao Y J , Rong R J , et al. Detection of GUS protein and its expression pattern in transgenic rice plants. Scientia Agricultura Sinica, 2014, 47 (14): 2715- 2722.
doi: 10.3864/j.issn.0578-1752.2014.14.002 |
|
汤浩茹, 王永清, 任正隆. 核桃体细胞胚发生与转基因研究进展. 林业科学, 2000, 36 (3): 102- 109.
doi: 10.3321/j.issn:1001-7488.2000.03.017 |
|
Tang H R , Wang Y Q , Ren Z L . An overview of progress on somatic embryogenesis and transformation in walnut. Scientia Silvae Sinicae, 2000, 36 (3): 102- 109.
doi: 10.3321/j.issn:1001-7488.2000.03.017 |
|
王倩倩, 常腾飞, 师校欣, 等. 外源基因在苹果转基因植株的稳定性研究. 分子植物育种, 2013, 11 (4): 539- 544. | |
Wang Q Q , Chang T F , Shi X X , et al. Studies on stability of exogenous gene in transgenic apples (Malus domestica Borkh.). Molecular Plant Breeding, 2013, 11 (4): 539- 544. | |
王文旭, 郁飞. 红色荧光蛋白融合表达载体的构建和蛋白质细胞内定位研究. 江苏农业科学, 2019, 47 (8): 60- 63. | |
Wang W X , Yu F . Construction of red fluorescent protein fusion expression vector and intracellular localization of protein. Jiangsu Agricultural Sciences, 2019, 47 (8): 60- 63. | |
吴瑞, 张树珍. 绿色荧光蛋白及其在植物分子生物学中的应用. 分子植物育种, 2005, 3 (2): 240- 244.
doi: 10.3969/j.issn.1672-416X.2005.02.014 |
|
Wu R , Zhang S Z . Green fluorescent protein and its application in plant molecular biology. Molecular Plant Breeding, 2005, 3 (2): 240- 244.
doi: 10.3969/j.issn.1672-416X.2005.02.014 |
|
夏兰芹, 王远, 郭三堆. 外源基因在转基因植物中的表达与稳定性. 生物技术通报, 2000, (3): 8- 12.
doi: 10.3969/j.issn.1002-5464.2000.03.002 |
|
Xia L Q , Wang Y , Guo S D . The stability of the expression of foreign genes in transgenic plants. Biotechnology Information, 2000, (3): 8- 12.
doi: 10.3969/j.issn.1002-5464.2000.03.002 |
|
杨晓玫, 师尚礼. 红、黄、绿三种颜色荧光质粒导入大肠杆菌中的稳定性表达. 甘肃农业大学学报, 2018, 53 (3): 193- 198. | |
Yang X M , Shi S L . Gene expression of red, yellow and green fluorescence plasmid stability after transferred in Escherichia coli. Journal of Gansu Agricultural University, 2018, 53 (3): 193- 198. | |
杨晓玫, 姚拓, 师尚礼. 荧光蛋白标记研究进展. 草业学报, 2019, 28 (10): 209- 216.
doi: 10.11686/cyxb2019020 |
|
Yang X M , Yao T , Shi S L . Progress in fluorescent protein labeling. Acta Prataculturae Sinica, 2019, 28 (10): 209- 216.
doi: 10.11686/cyxb2019020 |
|
杨宇, 李江江, 王项, 等. 报告基因及其应用研究进展. 生命科学研究, 2011, 15 (3): 277- 282. | |
Yang Y , Li J J , Wang X , et al. Progresses on reporter gene and its application. Life Science Research, 2011, 15 (3): 277- 282. | |
曾凡锁, 钱晶晶, 康君, 等. 转基因白桦中GUS基因表达的定量分析. 植物学报, 2009, 44 (4): 484- 490.
doi: 10.3969/j.issn.1674-3466.2009.04.010 |
|
Zeng F S , Qian J J , Kang J , et al. Histochemical study of β-glucuronidase activity in transgenic birch. Bulletin of Botany, 2009, 44 (4): 484- 490.
doi: 10.3969/j.issn.1674-3466.2009.04.010 |
|
曾凡锁, 王晓风, 骆薇, 等. 转基因白桦的花粉活力及外源基因的遗传表达分析. 植物生理学通讯, 2008, 44 (6): 1082- 1086. | |
Zeng F S , Wang X F , Luo W , et al. Analysis of pollen vigor, inheritance and expression of foreign genes in transgenic birch (Betula platyphylla Suk.) pollen. Plant Physiology Communications, 2008, 44 (6): 1082- 1086. | |
赵彦平, 赵春海. 植物转基因育种的分析与研究. 生物技术通报, 2011, 12 (3): 78- 83. | |
Zhao Y P , Zhao C H . Research and analysis of transgenic breeding in plants. Biotechnology Bulletin, 2011, 12 (3): 78- 83. | |
周瑞金, 杜国强, 师校欣. 外源基因在苹果植株中稳定性研究. 东北农业大学学报, 2011, 42 (7): 97- 101.
doi: 10.3969/j.issn.1005-9369.2011.07.018 |
|
Zhou R J , Du G Q , Shi X X . Study on stability of exogenous gene in transgenic apple plantlets. Journal of Northeast Agricultural University, 2011, 42 (7): 97- 101.
doi: 10.3969/j.issn.1005-9369.2011.07.018 |
|
朱靖杰. 香蕉组织培养中变异的发生与控制途径. 果树科学, 1995, 12 (2): 120- 122. | |
Zhu J J . The occurrence and control of mutation in banana tissue culture. Journal of Fruit Science, 1995, 12 (2): 120- 122. | |
Alford S C , Abdelfattah A S , Ding Y , et al. A fluorogenic red fluorescent protein heterodimer. Chemistry & Biology, 2012, 19 (3): 353- 360. | |
Czymmek K J , Bourett T M , Sweigard J A , et al. Utility of cytoplasmic fluorescent proteins for live-cell imaging of Magnaporthe grisea in planta. Mycologia, 2002, 94 (2): 280- 289.
doi: 10.1080/15572536.2003.11833234 |
|
Dandekar A M , Mcgranahan G H , Vail P V , et al. High levels of expression of full-length cryIA (c) gene from Bacillus thuringiensis in transgenic somatic walnut embryos. Plant Science, 1998, 131 (2): 181- 193.
doi: 10.1016/S0168-9452(97)00256-2 |
|
Dong Q Y , Liang X F , Zhang W , et al. Fluorescent labeling of Colletotrichum fructicola nuclei based on a reporter gene knock-in strategy. Mycosystema, 2018, 37 (2): 166- 174. | |
El-Euch C , Jay-Allemand C , Pastuglia M , et al. Expression of antisense chalcone synthase RNA in transgenic hybrid walnut microcuttings; Effect on flavonoid content and rooting ability. Plant Molecular Biology, 1998, 38 (3): 467- 479.
doi: 10.1023/A:1006034709501 |
|
Guo Y , Li W , Sun H , et al. Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Journal of General Plant Pathology, 2012, 78 (4): 247- 254.
doi: 10.1007/s10327-012-0390-x |
|
Honma Y , Yamakawa T . High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter. Plant Cell Reports, 2019, 38 (11): 1417- 1426.
doi: 10.1007/s00299-019-02453-7 |
|
Jach G , Binot E , Frings S , et al. Use of red fluorescent protein from Discosoma sp. (DsRED) as a reporter for plant gene expression. The Plant Journal, 2001, 28 (4): 483- 491. | |
Jawed A , Cook J . Reporter genes:Application to the study of mammalian gene transcription. Analytical Biochemistry, 1990, 188 (2): 245- 254.
doi: 10.1016/0003-2697(90)90601-5 |
|
Liu X , Walawage S L , Leslie C A , et al. In vitro gene expression and mRNA translocation from transformed walnut (Juglans regia) rootstocks expressing DsRED fluorescent protein to wild-type scions. Plant Cell Reports, 2017, 36 (6): 877- 885.
doi: 10.1007/s00299-017-2116-1 |
|
Mcgranahan G H , Leslie C A , Uratsu S L , et al. Improved efficiency of the walnut somatic embryo gene transfer system. Plant Cell Reports, 1990, 8 (9): 512- 516.
doi: 10.1007/BF00820198 |
|
Meyer P . Transcriptional transgene silencing and chromatin components. Plant Molecular Biology, 2000, 43 (2/3): 221- 234.
doi: 10.1023/A:1006483428789 |
|
Nishizawa K , Kita Y , Kitayama M , et al. A red fluorescent protein, DsRed2, as a visual reporter for transient expression and stable transformation in soybean. Plant Cell Reports, 2006, 25 (12): 1355- 1361.
doi: 10.1007/s00299-006-0210-x |
|
Smit P , Raedts J , Portyanko V , et al. NSP1 of the GRAS protein family is essential for rhizobial nod factor-induced transcription. Science, 2005, 308 (5729): 1789- 1791.
doi: 10.1126/science.1111025 |
|
Stem M , Mol J , Kooter J . The silencing of genes in transgenic plants. Annals of Botany, 1997, 79 (1): 3- 12.
doi: 10.1006/anbo.1996.0295 |
|
Sun L , Alariqi M , Zhu Y , et al. Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding. The Crop Journal, 2018, 6 (4): 366- 376.
doi: 10.1016/j.cj.2018.05.002 |
|
Swartz H J . Field performance and phenotypic stability of tissue-culture-propagated strawberries. Journal American Society for Horticultural, 1981, 106, 667- 673. | |
Taylor C B . Comprehending cosuppression. The Plant Cell, 1997, 9 (8): 1245- 1249. | |
Webb C D , Decatur A , Teleman A , et al. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. Journal of Bacteriology, 1995, 177 (20): 5906- 5911.
doi: 10.1128/JB.177.20.5906-5911.1995 |
|
Wu T M , Lin K C , Liau W S , et al. A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.). Plant Molecular Biology, 2016, 90 (1/2): 107- 115. | |
Yanushevich Y G , Staroverov D B , Savitsky A P , et al. A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins. Febs Letters, 2002, 511 (1): 11- 14. | |
Yarbrough D , Wachter R M , Kallio K , et al. Refined crystal structure of DsRED, a red fluorescent protein from coral, at 2.0-Å resolution. Proceedings of the National Academy of Sciences, 2001, 98 (2): 462- 467.
doi: 10.1073/pnas.98.2.462 |
|
Zhang Q , Walawage S L , Tricoli D M , et al. A red fluorescent protein(DsRED) from Discosoma sp. as a reporter for gene expression in walnut somatic embryos. Plant Cell Reports, 2015, 34 (5): 861- 869.
doi: 10.1007/s00299-015-1749-1 |
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