单叶蔓荆种子休眠特性与解除方法

doi:10.11707/j.1001-7488.20201218

Abstract

Abstract:

Objective: Vitex rotundifolia, sandy plant and halophyte, naturally grows on the sandy beach coast front with arid and barren soil. In order to adapt to the adverse environment of strong wind and sea immersion, the seeds of V. rotundifolia have developed a deep dormancy. This study aimed to explore the dormancy characteristics of V. rotundifolia seed and the methods of breaking the seed dormancy, so as to provide theoretical basis to the propagation from seeds and large-scale production. Method: In this study, water absorption of the fruit and seed was measured. The sites and types of endogenous germination inhibitors were biologically identified, the mechanical barrier of the seed coat was broken with physical means (concentrated H₂SO₄, cutting peel), and the inhibitor concentration was reduced by low temperature stratification and GA₃ treatment to study the possible types of and cause for dormancy, and methods of breaking dormancy of V. rotundifolia. Result: The results showed that:1) Vitex rotundifolia had a thick pericarp, and the endocarp had a completely corked stone cell layer with a seed empty shell rate of 26%. The peel had obvious mechanical hindrance to the water absorption, and the seed had no water absorption barrier. 2) The water extract and methanol extract of the peel were able to significant inhibit the seed germination and seedling growth of Chinese cabbage. The inhibitory effect of water extract on the seed germination and seedling growth of Chinese cabbage was more obvious than that of methanol extract under the same concentration, that is, the activity of the water-soluble germination inhibitor is greater than that of the alcohol-soluble germination inhibitor. The GA₃ treatment of peel water extract and methanol extract could significantly improve the seed germination rate, root length and seedling height of Chinese cabbage. The water extract and methanol extract of the seeds did not significantly inhibit the seed germination and seedling growth of Chinese cabbage, indicating that the presence of water-soluble and alcohol-soluble germination inhibitors in the pericarp of V. rotundifolia is the main cause of seed dormancy. 3) Seed dormancy was able to be broken down by low temperature stratification for 90 d, GA₃, GA₃+concentrated H₂SO₄, GA₃+cutting peel, GA₃+low temperature stratification for 30 d, GA₃+low temperature stratification for 60 d and GA₃+low temperature stratification for 90 d. The seed germination time under GA₃+concentrated H₂SO₄ treatment was the shortest(14 days), and the germination rate was the highest(44%). Conclusion: The mechanical barrier and the endogenous inhibitor of the peel are the main factors affecting the dormancy of Vitex rotundifolia. Treatment with 500 mg·L^-1GA₃+concentrated H₂SO₄ can effectively release seed dormancy and increase germination rate.

Key words: Vitex rotundifolia, seed dormancy, mechanical obstruction, endogenous inhibitor, GA₃

CLC Number:

S722.1⁺4

Dejie Yin,Fengqin Bu,Yanfang Xu,Biao Huang,Cheng Li. Dormancy Characteristics and Breaking Methods of Vitex rotundifolia Seeds[J]. Scientia Silvae Sinicae, 2020, 56(12): 157-165.

Figures/Tables 7

Table 1

Different experimental treatments on the fruits of Vitex rotundifolia"

序号 No.	处理 Treatments
1	对照处理CK
2	1 mol·L^-1H₂SO₄浸泡15 min，用蒸馏水冲洗，并用纱布搓掉表面碳化物质Soaking for 15 min in 1 mol·L^-1 H₂SO₄, rinsing with distilled water, and removing the surface carbonized material with gauze
3	对果皮进行切割处理Cutting peel
4	500 mg·L^-1 GA₃溶液浸泡24 h Soaking in 500 mg·L^-1 GA₃ solution for 24 h
5	1:1.5的比例混合种子与湿沙，保持沙的含水率为60%并搅拌均匀，放置于4 ℃冰箱进行低温层积处理，层积天数为30天Mixing the seeds and wet sand in a ratio of 1:1.5, keeping the water content of the sand at 60% and stiring evenly, then placing it in a refrigerator at 4 ℃ for low-temperature stratification of 30 days
6	处理同5，层积天数为60天Processing the same as No.5, 60 days of stratification
7	处理同5，层积天数为90天Processing the same as No.5, 90 days of stratification
8	1 mol·L^-1浓H₂SO₄浸泡15 min，用蒸馏水冲洗，并用纱布搓掉表面碳化物质后，500 mg·L^-1 GA₃溶液浸泡24 h Soaking for 15 min in 1 mol·L^-1 H₂SO₄, rinsing with distilled water, soaking for 24 h with 500 mg·L^-1 GA₃ after removing the surface carbonized material with gauze
9	对果皮进行切割处理后，500 mg·L^-1 GA₃溶液浸泡24 h Soaking for 24 h with 500 mg·L^-1 GA₃ after cutting the peel
10	500 mg·L^-1 GA₃溶液浸泡24 h后，低温层积设置梯度30天(低温层积方法同5) Low-temperature stratification of 30 days after soaking for 24 h with 500 mg·L^-1 GA₃
11	500 mg·L^-1 GA₃溶液浸泡24 h后，低温层积设置梯度60天(低温层积方法同5) Low-temperature stratification of 60 days after soaking for 24 h with 500 mg·L^-1 GA₃
12	500 mg·L^-1 GA₃溶液浸泡24 h后，低温层积设置梯度90天(低温层积方法同5)Low-temperature stratification of 90 days after soaking for 24 h with 500 mg·L^-1 GA₃

Table 1

Fig.1

Fig.2

Fig.3

Table 2

Table 3

Table 4

References 0

	曹晓晓, 王奎玲, 刘庆超, 等. 2011.单叶蔓荆繁殖方式研究.银川: 中国园艺学会观赏园艺专业委员会2011年学术年会.
	Cao X X, Wang K L, Liu Q C, et al. 2011. Research on reproduction pattern of Vitex trifolia var. simplicifolia. Yinchuan: China Horticultural Society Ornamental Horticulture Committee 2011 Academic Annual Meeting.[in Chinese]
	常宗涛, 胡胜云, 杜楠楠, 等. 沙质海岸防护林树种单叶蔓荆个体生态学研究综述. 安徽农业科学, 2016, 44 (27): 160- 161. doi: 10.3969/j.issn.0517-6611.2016.27.055
	Chang Z T , Hu S Y , Du N N , et al. Review of autecology of Vitex trifolia var. simplicifolia for coastal sandy shelter forest. Journal of Anhui Agricultural Sciences, 2016, 44 (27): 160- 161. doi: 10.3969/j.issn.0517-6611.2016.27.055
	陈怀远, 涂林锋, 肖春荣, 等. 单叶蔓荆子的化学成分研究. 中国中药杂志, 2018, 43 (18): 3694- 3700.
	Chen H Y , Tu L F , Xiao C R , et al. Chemical constituents from fruits of Vitex trifolia var. simplicifolia. China Journal of Chinese Materia Medica, 2018, 43 (18): 3694- 3700.
	付婷婷, 程红焱, 宋松泉. 种子休眠的研究进展. 植物学报, 2009, 44 (5): 629- 641. doi: 10.3969/j.issn.1674-3466.2009.05.014
	Fu T T , Cheng H Y , Song S Q . Advances in studies of seed dormancy. Chinese Bulletin of Botany, 2009, 44 (5): 629- 641. doi: 10.3969/j.issn.1674-3466.2009.05.014
	洑香香, 周晓东, 刘红娜, 等. 木本植物种子休眠和萌发的分子生物学研究综述. 世界林业研究, 2011, 24 (4): 24- 29.
	Fu X X , Zhou X D , Liu H N , et al. Review of seed dormancy and germination of woody plant in molecular biology. World Forestry Research, 2011, 24 (4): 24- 29.
	古今, 吴梅, 李文春, 等. 滇重楼种子中萌发抑制物质活性的研究. 现代中药研究与实践, 2013, (1): 10- 12.
	Gu J , Wu M , Li W C , et al. Study on the activity of germination inhibitors in the seeds of Paris polyphylla var. yunnanensis. Research and Practice of Chinese Medicines, 2013, (1): 10- 12.
	郭聪聪, 沈永宝, 史锋厚. 白皮松种子休眠研究进展. 南京林业大学学报:自然科学版, 2019, 43 (2): 175- 183.
	Guo C C , Shen Y B , Shi F H . Review on seed dormancy of Pinus bungeana Zucc. Journal of Nanjing Forestry University:Natural Sciences Edition, 2019, 43 (2): 175- 183.
	黄玮, 孟繁蕴, 张文生, 等. 滇重楼种子休眠机理研究. 中国农学通报, 2008, 24 (12): 242- 246.
	Huang W , Meng F Y , Zhang W S , et al. Study on seed dormancy mechanism of Paris polyphylla var. yunnanensis. Chinese Agricultural Science Bulletin, 2008, 24 (12): 242- 246.
	刘超, 伏秦超, 罗正敏, 等. 肉果秤锤树核果中萌发抑制物质的初步研究. 北方园艺, 2013, (4): 20- 24.
	Liu C , Fu Q C , Luo Z M , et al. Preliminary study on germination inhibitors from Sinojackia sarcocarpa drupe. Northern Horticulture, 2013, (4): 20- 24.
	潘昊磊, 赵毅, 石文昊, 等. 牡丹种子休眠与萌发综述. 现代农业科技, 2019, (20): 128- 129, 131. doi: 10.3969/j.issn.1007-5739.2019.20.076
	Pan H L , Zhao Y , Shi W H , et al. A review of peony seed dormancy and germination. Modern Agricultural Science and Technology, 2019, (20): 128- 129, 131. doi: 10.3969/j.issn.1007-5739.2019.20.076
	乔勇进, 张敦论, 郗金标, 等. 沿海沙质海岸单叶蔓荆群落特点及土壤改良的分析. 防护林科技, 2001, (4): 6- 8. doi: 10.3969/j.issn.1005-5215.2001.04.002
	Qiao Y J , Zhang D L , Xi J B , et al. Analysis of soil improvement and community features of Vitex trifolia var. simplicifolia on the sandy coast. Protection Forest Science and Technology, 2001, (4): 6- 8. doi: 10.3969/j.issn.1005-5215.2001.04.002
	尚旭岚, 徐锡增, 方升佐. 青钱柳种子休眠机制. 林业科学, 2011, 47 (3): 68- 74.
	Shang X L , Xu X Z , Fang S Z . Seed dormancy mechanism of Cyclocarya paliurus. Scientia Silvae Sinicae, 2011, 47 (3): 68- 74.
	苏贺. 2016.巫山淫羊藿种子休眠机制及解除方法研究.北京: 中国农业大学博士学位论文.
	Su H. 2016. Study on seed dormancy mechanism and dissolution method of Epimedium wushanense. Beijing: PhD thesis of China Agricultural University.[in Chinese]
	孙荣进, 罗光明. 单叶蔓荆种子休眠特性研究. 中草药, 2012, 43 (8): 1621- 1625.
	Sun R J , Luo G M . Seed dormancy characteristics for Vitex trifolia var. simplicifolia. Chinese Traditional and Herbal Drugs, 2012, 43 (8): 1621- 1625.
	孙婷, 刘鹏, 徐根娣. 栝楼种子休眠特性分析. 中国农业科学, 2008, 41 (12): 4273- 4280. doi: 10.3864/j.issn.0578-1752.2008.12.044
	Sun T , Liu P , Xu G D . Study on dormancy characteristics of Trichosathes kirilowii Maxim. Scientia Agricultura Sinica, 2008, 41 (12): 4273- 4280. doi: 10.3864/j.issn.0578-1752.2008.12.044
	孙云波, 陈翔, 陈垣, 等. 种荚预处理对红芪种子发芽特性的影响. 草业学报, 2015, 24 (6): 159- 167.
	Sun Y B , Chen X , Chen H , et al. Effects of pod pre-treatments on the germination traits of Hedysarum polybotrys seeds. Acta Prataculturae Sinica, 2015, 24 (6): 159- 167.
	唐安军. 中国特有植物凹叶木兰种子的休眠与贮藏行为. 植物生理学报, 2014, 50 (1): 105- 110.
	Tang A J . Seed dormancy and storage behavior of Magnolia sargentiana endemic to China. Plant Physiology Journal, 2014, 50 (1): 105- 110.
	王世杰, 莫颖宁. 栝楼种子休眠与促萌发研究进展. 中医药导报, 2018, 24 (15): 118- 120.
	Wang S J , Mo Y N . Research progress of dormancy and germination promotion of Gualou(Trichosanthes Kirilowii Maxim)seed. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2018, 24 (15): 118- 120.
	王小平, 王九龄. 白皮松种子内含物的提取、分离及生物测定. 种子, 1998, 17 (5): 19- 22.
	Wang X P , Wang J L . Extraction, separation and biological measurement of endogenous growth regulating substance of seeds of Pinus bungeana. Seed, 1998, 17 (5): 19- 22.
	魏宗贤, 周赛霞, 彭焱松, 等. 鄱阳湖沙地沙漠化过程中单叶蔓荆群落结构和功能特征. 广西植物, 2011, 31 (5): 620- 626. doi: 10.3969/j.issn.1000-3142.2011.05.011
	Wei Z X , Zhou S X , Peng Y S , et al. Characterication of construction and functions of Vitex trifolia var. simplicifolia community during the process of desertification in Poyang Lake. Guihaia, 2011, 31 (5): 620- 626. doi: 10.3969/j.issn.1000-3142.2011.05.011
	徐向东, 董丽莉, 卢晓燕, 等. 黄山花楸果实水浸提液的萌发抑制作用. 安徽农业科学, 2014, 42 (29): 10183- 10184. doi: 10.3969/j.issn.0517-6611.2014.29.053
	Xu X D , Dong L L , Lu X Y , et al. The inhibition of aqueous extracts of Sorbus amabilis fruit on seeds germination. Journal of Anhui Agricultural Sciences, 2014, 42 (29): 10183- 10184. doi: 10.3969/j.issn.0517-6611.2014.29.053
	闫芳, 张恩和, 王勤礼, 等. 野生黄瑞香种子的休眠机制与破除方法. 林业科学, 2016, 52 (4): 30- 37.
	Yan F , Zhang E H , Wang Q L , et al. The mechanisms of seed dormancy of wild Daphne giraldii and methods for dormancy breaking. Scientia Silvae Sinicae, 2016, 52 (4): 30- 37.
	颜启传. 种子学. 北京: 中国农业出版社, 2001: 64- 71.
	Yan Q C . Seeds. Beijing: China Agricultural Press, 2001: 64- 71.
	颜升, 罗光明, 朱玉野, 等. 栀子种子萌发条件优化及萌发抑制物的初步研究. 南方农业学报, 2014, 45 (3): 376- 382. doi: 10.3969/j.issn.2095-1191.2014.3.376
	Yan S , Luo G M , Zhu Y Y , et al. Optimization of seed germination and detection of germination inhibitors in gardenia fruits. Journal of Southern Agriculture, 2014, 45 (3): 376- 382. doi: 10.3969/j.issn.2095-1191.2014.3.376
	于卓, 王林和. 三种沙拐枣种子休眠原因研究初探. 西北林学院学报, 1998, 13 (3): 9- 13.
	Yu Z , Wang L H . Causes of seed dormancy of three species of Calligonum. Journal of Northwest Forestry University, 1998, 13 (3): 9- 13.
	张丹, 张金政, 孙国峰, 等. 细叶鸢尾种子休眠原因及解除休眠的方法研究. 草业学报, 2011, 20 (6): 109- 117.
	Zhang D , Zhang J Z , Sun G F , et al. A study on the causes of Iris tenuifolin dormancy and the methods for breaking dormancy. Acta Prataculturae Sinica, 2011, 20 (6): 109- 117.
	张瑞, 李廷山, 胡小文, 等. 窄叶野碗豆种子的休眠与发芽特性. 西北植物学报, 2014, 34 (1): 135- 142.
	Zhang R , Li T S , Hu X W , et al. Seed dormancy and germination characteristics of Vicia angustifolia. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34 (1): 135- 142.
	朱敏嘉, 黄俊华, 杨文英, 等. 裕民贝母种子形态休眠解除过程中营养物质的动态变化. 江苏农业科学, 2019, 47 (11): 182- 186.
	Zhu M J , Huang J H , Yang W Y , et al. Dynamic changes of nutrients during Fritillaria imperialis seed form dormancy release. Jiangsu Agricultural Sciences, 2019, 47 (11): 182- 186.
	Baskin C C, Baskin J M. 2004. Determining dormancy-breaking and germination requirements from the fewest seeds. Washington: Island Press, 162-179.
	Baskin J M , Baskin C C . A classification system for seed dormancy. Seed Science Research, 2004, 14 (1): 1- 16. doi: 10.1079/SSR2003150
	Carrick K M , Plyler D B . Interaction of fusicoccin and abscisic acid in breaking and restoring seed dormancy in Spartina alterniflora. ASB Bulletin Association of Southeastern Biologists, 1994, 41 (2): 82.
	Debeaujon I , Koornneef M . Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid. Plant Physiology, 2000, 122 (2): 415- 424. doi: 10.1104/pp.122.2.415
	Gusano M G , Gomez P M , Dicenta F . Breaking seed dormancy in almond (Prunus dulcis (Mill.) D.A.Webb). Scientia Horticulturae, 2004, 99 (1): 363- 370.
	Kermode A R . Role of abscisic acid in seed dormancy. Journal of Plant Growth Regulation, 2005, 24 (7): 319- 344.
	Nikolaeva M G . Factors controlling the seed dormancy pattern. Physiology and Biochemistry of Seed Dormancy and Germination, 1977, 57 (3): 51- 74.
	Roberts E H . Seeds:Physiology of development and germination. Plant, Cell and Environment, 1986, 9 (4): 356.
	Walck J L , Hidayati S N , Okagami N . Seed germination ecophysiology of the Asian species Osmorhiza aristata (Apiaceae):comparison with its North American congeners and implications for evolution of types of dormancy. American Journal of Botany, 2002, 89 (5): 829- 835. doi: 10.3732/ajb.89.5.829

浸提液 Extracts solvent	浓度Concentration/(g·mL^-1)	发芽率 Germination percentage(%)	主根长 Root length/cm	苗高 Seedling height/cm	内源萌发抑制活性 Germination inhibition activity
CK	—	63.33±2.60 a	2.13±0.37 a	2.87±0.39 a
水Water	0.01	23.00±2.00 b	1.35±0.060 b	2.08±0.45 a	63.68±3.16 b
	0.05	22.33±1.20 b	1.35±0.12 b	2.03±0.13 b	64.73±1.90 b
	0.1	19.67±3.48 c	1.33±0.41 b	1.99±0.081 b	68.95±5.50 a
甲醇Methanol	0.01	24.67±0.67 b	1.75±0.059 ab	2.45±0.094 ab	61.05±1.05 b
	0.05	23.98±1.20 b	1.39±0.18 b	2.07±0.16 b	62.13±1.90 b
	0.1	22.33±1.45 b	1.31±0.14 b	2.01±0.10 b	64.73±2.29 a

浸提液 Extracts solvent	浓度Concentration/(g·mL^-1)	发芽率 Germination percentage (%)	主根长 Root length/cm	苗高 Seedling height/cm
CK	—	63.33±2.60 a	2.13±0.37 a	2.87±0.39 a
水Water	0.01	46.25±1.36 b	1.54±0.090 b	2.26±0.29 ab
	0.05	38.74±0.97 b	1.48±0.15 b	2.20±0.37 b
	0.10	34.19±1.12 b	1.43±0.26 b	2.01±0.092 b
甲醇Methanol	0.01	51.82±2.73 b	1.87±0.13 ab	2.57±0.087 a
	0.05	42.59±1.72 b	1.69±0.17 b	2.21±0.19 ab
	0.10	38.65±0.65 b	1.57±0.078 b	2.18±0.13 ab

处理 Treatment	发芽开始天数 Days beginning/d	发芽率 Germination percentage(GR)(%)	发芽势 Germination potential(GP)(%)	发芽指数 Germination index (GI)	活力指数 Vitality index (Ⅵ)
CK	0	0	0	0	0
1 mol·L^-1H₂SO₄1 mol·L^-1H₂SO₄	0	0	0	0	0
切割果皮Cutting peel	0	0	0	0	0
低温层积30天Low temperature layering 30 d	0	0	0	0	0
低温层积60天Low temperature layering 60 d	0	0	0	0	0
低温层积90天Low temperature layering 90 d	25	16.00±1.15 d	12.00±1.15 c	3.20±0.17 f	1.28±0.086 g
GA₃	18	22.67±0.67 c	15.33±0.67 bc	6.84±0.17 c	4.96±0.058 c
GA₃+1 mol·L^-1浓H₂SO₄ GA₃+1 mol·L^-1 H₂SO₄	14	44.00±1.15 a	28.67±1.76 a	8.92±0.17 a	8.33±0.075 a
GA₃+切割果皮GA₃+ Cutting peel	15	33.33±1.76 b	20.00±2.31 b	6.13±0.17 d	3.50±0.18 d
GA₃+低温层积30天GA₃+low temperature layering 30 d	19	19.33±1.76 cd	14.00±1.15 c	4.17±0.27 e	1.75±0.055 f
GA₃+低温层积60天GA₃+low temperature layering 60 d	19	21.33±1.76 c	16.00±1.15 bc	4.34±0.097 e	2.16±0.21 e
GA₃+低温层积90天GA₃+low temperature layering 90 d	17	36.00±1.15 b	24.67±1.76 a	8.34±0.16 b	7.25±0.10 b

[1]	Chen Xiangbo, Liu Yang, Zhao Mingshui, Tu Shuping. Embryo Development and Dormancy Releasing of Acer yangjuechi, the Extremely Endangered Plant [J]. Scientia Silvae Sinicae, 2017, 53(4): 65-73.
[2]	Yan Fang, Zhang Enhe, Wang Qinli, Mao Zhuhong. The Mechanisms of Seed Dormancy of Wild Daphne giraldii and Methods for Dormancy Breaking [J]. Scientia Silvae Sinicae, 2016, 52(4): 30-37.
[3]	Zhu Zhenjia, Jiang Chengying, Shi Yanhu, Chen Weiqing, Chen Nianlai, Zhao Mengjiong, Wu Wenjun. Variations of Endogenous Hormones in Lateral Buds of Olive Trees(Olea europaea) during Floral Induction and Flower-Bud Differentiation [J]. Scientia Silvae Sinicae, 2015, 51(11): 32-39.
[4]	Ma Hong;Li Zhenghong;Zhang Yanli;Wang Yan;Liu Xiuxian;Wan Youming. Release of Seed Dormancy of Paeonia ludlowii [J]. Scientia Silvae Sinicae, 2012, 48(9): 62-67.
[5]	Wei Zhigang;Qian Tingting;Zhang Kaixuan;Liu Guanjun;Liu Guifeng;Yang Chuanping. Effect of Exogenous GA₃ on Flowering Genes Expression of Betula platyphylla by Real-Time Fluorescent Quantitative PCR [J]. Scientia Silvae Sinicae, 2011, 47(7): 187-192.
[6]	Shang Xulan;Xu Xizeng;Fang Shengzuo. Seed Dormancy Mechanism of Cyclocarya paliurus [J]. Scientia Silvae Sinicae, 2011, 47(3): 68-74.
[7]	Zhang Rumin;Bai Jing;Lü Chunling;Chen Hongwei;Gao Yan. Fluctuating-Temperature Stratification Induced Seed Germination of Cistanche deserticola [J]. Scientia Silvae Sinicae, 2008, 44(9): 170-173.
[8]	Qian Hua;Liu Yan;Yu Jiying;Fan Wenfeng;Zhang Ying. Effects of Different Hormone on the Flowering of Two Nobile-Type Dendrobium Cultivars [J]. Scientia Silvae Sinicae, 2007, 43(8): 148-150.
[9]	Shen Hailong;YangLing;Zhang Jianying;Feng Dandan;Fan Shaohui. Influencing Factors to Seed Dormancy and Germination Characteristics of Sorbus pohuashanensis [J]. Scientia Silvae Sinicae, 2006, 42(10): 133-138.
[10]	Dong Lifen;Shao Chongbin;Zhang Zongqin. CHARACTERISTICS OF EMBRYO DORMANCY AND GERMINATION OF PINUS BUNGEANA [J]. Scientia Silvae Sinicae, 2003, 39(6): 47-54.
[11]	Pei Dong;Zhang Junpei;Shi Yongsen;Xu Huzhi. SEED GERMINATION AND SEEDLING GROWTH ASSOCIATED WITH STRATIFICATION WAYS ON JUGLANS NIGRA [J]. Scientia Silvae Sinicae, 2002, 38(5): 73-77.
[12]	Kongshu Ji,Zhangrong Wang,Tianhua Chen,Mingxiu Wang. STUDY ON THE ENDOGENOUS INHIBITORS IN MASSON PINE (PINUS MASSONIANA LAMB.) CUTTINGS [J]. Scientia Silvae Sinicae, 1997, 33(2): 142-151.
[13]	Xu Huacheng;Tang Jilin. SEED GERMINATION ECOLOGY OF PINUS TABULAEFORMIS IN RELATION TO THEIR PROVENANCES [J]. , 1989, 25(6): 492-501.

Dormancy Characteristics and Breaking Methods of Vitex rotundifolia Seeds

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