红枝鸡爪槭枝枯病病原鉴定及防治

doi:10.11707/j.1001-7488.20140616

Abstract

Abstract:

To explore the pathogens of branch blight of Acer palmatum ‘Sangokaku’ in Zhejiang province,The infected samples were collected from Siming mountain area of Zhejiang in September 2013. The pathogens were isolated by tissue isolation method from blighted A. palmatum‘Sangokaku’ branch and cultured on standard medium. The obtained colonies were identified as Colletotrichum according to the taxonomic system of Sutton B C after being subcultured and single hyphal fragment. The results showed that the six isolates were obtained from the sampling sites. Their conidia were cylindrical, colorless single spore, with one oil ball, blunt ends, ranged from (18.23-11.76) μm × (4.46-2.94) μm (average 14.79 μm × 4.06 μ m). The appressoria were nearly round or irregular in shape, edge smoothing, pale brown, average 8.67 μm × 4.52 μ m. Conidia and appressoria were similar with Colletotrichum gloeosporioides in shape and size. Pathogenicity tests were performed by strain Z920121. After inoculation, the same branch blight was observed in 23 days on 100% of inoculated branches. DNA was directly extracted from the mycelium of Z920121 grown on PDA for 10 days. The identities of the isolates were confirmed by ITS1-5.8S-ITS2 rDNA sequence (GenBank Accession No. KF787111) analysis that showed 100% sequence similarity to C. gloeosporioides. (Accession No. KC493156.1,HQ645082.1). It was identified that the pathogen of branch blight of A.palmatum ‘Sangokaku’ in Zhejiang province was C. gloeosporioides. Treatment by spraying the plant with 40% EC 800-times diluted prochloraz liquid had a control effect on the disease up to 92.6%.

Key words: Acer palmatum‘Sangokaku’, branch blight, pathogen, Colletotrichum gloeosporioides, control

CLC Number:

S763.1

Wang Zhilong, Lin Li, Wang Guoliang, Zhu Zhiyong, Xu Wanwan. Identification of Pathogen and Control of Acer palmatum ‘Sangokaku’ Branch Blight[J]. Scientia Silvae Sinicae, 2014, 50(6): 125-130.

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Identification of Pathogen and Control of Acer palmatum ‘Sangokaku’ Branch Blight

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