Welcome to visit Scientia Silvae Sinicae，Today is

Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (3): 136-142.doi: 10.11707/j.1001-7488.20120323

Previous Articles     Next Articles

Effects of Pulsed Magnetic Field on the Biochemical and Physiological Characters and Ultrastructure of Pine Wood Nematode

Zhang Ming1,2, Song Xiaobin2, Zhang Xingyao1, Wang Zhenchao3, Liang Jun1   

  1. 1. Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091;2. College of Forestry, Northwest A&F University Yangling 712100;3. College of Electronic and Information Engineering, Hebei University Baoding 071002
  • Received:2011-05-24 Revised:2011-09-12 Online:2012-03-25 Published:2012-03-25

PDF (PC)

528

Abstract:

In this paper, we employed the pulsed magnetic field (PMF) processing to investigate the effect of different pulse rise time and treatment time on the survival, reproduction, cellulase activity and surface characteristics of the pine wood nematode (PWN). The results showed that the wider pulse and the longer treatment time were able to reduce the survival, and inhibit the cellulase activity and reproduction of PWN. The 4th instar and females had a strong resistance to the pulsed magnetic field. With pulse rise time of 10 ms and the treatment of 60 min, the adjusted mortality of PWN was 100%. After the PMF treatment, the cellulase activity of PWN increased within a short time, and then declined with the treatment time. The observation with a scanning electron micrograph (SEM) revealed that there was no significant change in morphology of PWN, but the quantity of rod-shaped bacteria carried by PWN was reduced.

Key words: pulsed magnetic field, pulse rise time, cellulase activity, mortality rate, pine wood nematode

CLC Number: