Abstract:

Pseudomonas sp. was mutated by ultraviolet (UV). Then the UV mutated Pseudomonas sp. was immobilized on bamboo-carbon to investigate its effect in the removal of m-cresol. The effects of various parameters, such as amount of immobilized mutant Pseudomonas sp. and pH, on m-cresol removal were examined. Meanwhile, the m-cresol removal kinetics and the relationship between concentration and reaction time were obtained. The results show that compared with wild strain, the growth cycle of the strain which is treated by UV mutation is shortened by 6 h. Pseudomonas irradiated by UV for 120 s can form obvious zoogloea on the bamboo-carbon surface area and inner pore, and the biomass generated by the mutant on the bamboo-carbon is significantly larger than that by the wild strain. Parameters such as immobilized biomass and pH affect the removal of m-cresol. The removal effect is better with pH at the range of 4 to 6. When 20 g of bamboo-carbon immobilized UV mutated Pseudomonas sp. is used to treat 100 mL of simulated m-cresol water sample, with its initial concentration being 50, 100, 120, 150, 180 mg·L^-1, removal effect is respectively 90.9%,76.4%,72.9%,64.6% and 49.7% after 42 h and the removal of m-cresol follows zero-order kinetics.

Key words: bamboo-carbon, immobilization, UV mutation, Pseudomonas sp., m-cresol