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Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (6): 108-113.doi: 10.11707/j.1001-7488.20110616

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Morphological Type and Molecular Identification of Ectomycorrhizae on Picea crassifolia in Helan Mountain

Fan Yongjun1, Yan Wei2, Wang Liyuan1   

  1. 1. Department of Biology Science and Technology, Baotou Teachers College Baotou 014030;2. Forest College, Inner Mongolia Agricultural University Huhhot 010018
  • Received:2009-11-18 Revised:2010-05-19 Online:2011-06-25 Published:2011-06-25

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Abstract:

A preliminary study on diversity of ectomycorrhizas associated with Picea crassifolia at Helan Mountain area was investigated using morphological, anatomical and molecular characteristics, of PCR amplified product analysis of the ITS region of rDNA, and then identification results were obtained according to that the sequences were BLASTed using the GenBank. The results indicated that there were 11 different ECM types on Picea crassifolia in Helan Mountain. ITS regions of rDNA of the 11 different ECM fungi were amplified and sequenced, and the sequences were compared with those registered in DNA sequence database on Internet, identified to family level of 2, genus level of 2 and species level of 7, respectively. Among the 11 identified ectomycorrhizal types, 2 species belong to Ascomycotina and the rest species belong to Basidiomycotina. It was found that using sequences of rDNA ITS regions confirmed consistently the classification of these species based on morphological and anatomic ways.

Key words: Picea crassifolia, ectomycorrhizae, morphological and anatomical characteristics, molecular identification

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