Abstract:

It is hardly possible to isolate nucellus in its original shape from tiny ovules of Populus tomentosa without enzymatic maceration. This paper introduced a new method to isolate the nucellus from the ovule of P.tomentosa with gum block, the anatomical lens and other ordinary instruments. The isolated nucellus was little damaged and could maintain its shape. The technique made the nucellus easy to isolate, and can be used for non-enzymatically isolating the ovule, nucellus and embryo of flowering plants. These works can be finished at a laboratory even in a reduced condition.

Key words: Populus tomentosa, ovule, nucellus, gum block, isolate