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Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (4): 37-42.doi: 10.11707/j.1001-7488.20100406

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Isolation and Analysis of Stowaway-like MITEs from DNA Libraries Enriched in Phyllostachys edulis

Zhong Hao1,Zhou Mingbing1,Bai Youhuang2,Tang Dingqin1   

  1. 1. Zhejiang Key Laboratory for Modern Silvicultural Technology Zhejiang Forestry University Lin’an 311300；2. Department of Bioinformatics, College of Life Sciences, Zhejiang University Hangzhou 310058
  • Received:2009-08-31 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

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Abstract:

Transposable elements were a kind of DNA fragments movable in genomes. Miniature inverted repeat transposable elements (MITEs) were a class of short, widespread transposable elements with high copy number which played an important role in evolution of genome and regulation of gene expression. In order to analyze the distribution and characteristics of MITEs in Phyllostachys edulis genome, DNA library enriched for Stowaway-like MITEs was firstly constructed by the method of FIASCO (Fast Isolation by AFLP of Sequences Containing repeats). Totally 21 recombinant clones were randomly isolated with PCR method and then sequenced. Two clones (Stow-Ph1 and Stow-Ph2) were identified as Stowaway-like MITEs. The rate of positive clones was 9.52%. The fragment lengths of Stow-Ph1 and Stow-Ph2 were 260 bp and 258 bp, respectively. They had typical TIRs (terminal inverted repeats) and TSD (target site duplication) of Stowaway-like MITEs. The identity was 46.4% between Stow-Ph1and Stow-Os8(FJ266024), and 52.6% between Stow-Ph2and Stow-Os8. The identities among Stow-Ph1,Stow-Ph2and 16 rice Stowaway-like MITEs were above 50% based on the first 20 bp of their TIRs, which reflected that the TIRs of Stow-Ph1 and Stow-Ph2 were highly conserved.

Key words: Phyllostachys edulis, enrichment by magnetic beads, transposon, TIRs, Stowaway-like MITEs