松材线虫纤维素酶的免疫学分析

doi:10.11707/j.1001-7488.20070211

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (02): 64-67.doi: 10.11707/j.1001-7488.20070211

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Immunological Analysis of Bursaphelenchus xylophilus Pathogenous Cellulase

Zhang Qi¹,Li Haiyan²,Bai Gang¹,Xiong Huilong²,Yang Wenbo¹

1. College of Life Science, Nankai University Tianjin 300071; 2. General Station of Forest Pest Control, State Administration of Forestry Shenyang 110034

Received:2005-07-28 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract:

After producing mouse anti-cellulase BXC60 polyclonal antibody (anti-BXC60), the specificity of Anti-BXC60 was verified by SDS-PAGE, cellulase staining, western blot and ELISA method. Further, we established a double staining method with rabbit anti-Bursaphelenchus xylophilus serum (Anti-BX) and Anti-BXC60, which could be used for analyzing secretory proteins on PVDF membrane. The result of double staining assay showed that the pine wood nematodes secreted some solute proteins through secretion pore, and cellulase was secreted from stylet that was the same as other plant nematodes.

Key words: Bursaphelenchus xylophilus, cellulase, double staining

Zhang Qi;Li Haiyan;Bai Gang;Xiong Huilong;Yang Wenbo. Immunological Analysis of Bursaphelenchus xylophilus Pathogenous Cellulase[J]. Scientia Silvae Sinicae, 2007, 43(02): 64-67.

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Immunological Analysis of Bursaphelenchus xylophilus Pathogenous Cellulase

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