美洲黑杨雄性花芽cDNA克隆测序及表达序列标签(ESTs)特性分析

doi:10.11707/j.1001-7488.20061107

Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (11): 37-41.doi: 10.11707/j.1001-7488.20061107

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Sequencing of cDNA Clones and Analysis of the Expressed Sequenced Tags(ESTs)Properties of Male Floral Buds of Populus deltoides

Zhou Xiangming^1,2,Zhang Bingyu²,Su Xiaohua²,Wang Dahai²,Huang Qinjun²,Zhang Xianghua²,Zhang Zhiyi¹

1.Key Laboratory for Genetic and Breeding of Forest Tree and Ornamental Plants,Ministry of Education Beijing Forestry University Beijing 100083;2.Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration Research Institute of Forestry,CAF Beijing 100091

Received:2005-09-27 Revised:1900-01-01 Online:2006-11-25 Published:2006-11-25

Abstract

Abstract:

A cDNA library of male floral buds of Populus deltoides through SMART(the Switch Mechanism At the 5' end of RNA Templates)technique was constructed.4 200 clones randomly selected from the cDNA library were sequenced and 3 092 raw sequences were obtained.3 087 valid ESTs were generated after the size of inserts shorter than 150 bp and the contaminants were removed.The average length of the cDNA was 515 bp.A total of 416 clusters were formed after assembly of the 3 087 ESTs,and 451 contigs and 1 104 singletons were obtained.1 015 ESTs with known or unknown function were identified by BlastN searched against the NCBI non_redundant nucleotide databases,540 ESTs had no significant similarity to any protein or DNA sequence in the databases.The present ESTs provide a valuable resource for further research on the flower development of higher plants.

Key words: Populus deltoides, male floral bud, cDNA library, ESTs

Zhou Xiangming;Zhang Bingyu;Su Xiaohua;Wang Dahai;Huang Qinjun;Zhang Xianghua;Zhang Zhiyi. Sequencing of cDNA Clones and Analysis of the Expressed Sequenced Tags(ESTs)Properties of Male Floral Buds of Populus deltoides[J]. Scientia Silvae Sinicae, 2006, 42(11): 37-41.

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Sequencing of cDNA Clones and Analysis of the Expressed Sequenced Tags(ESTs)Properties of Male Floral Buds of Populus deltoides

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