松材线虫rDNA-ITS2的Taq Man探针实时荧光PCR检测

doi:10.11707/j.1001-7488.20050213

Scientia Silvae Sinicae ›› 2005, Vol. 41 ›› Issue (2): 82-85.doi: 10.11707/j.1001-7488.20050213

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Studies on Real-Time Fluorescent PCR With TaqMan Probe for rDNA-ITS2 of Pine Wood Nematode(Bursaphelenchus xylophilus)

Wang Mingxu^1,2,Zhu Shuifang³,Luo Kuan¹,Zhou Lihua¹,Zhao Wenjun³

1.College of Bio-safety Science and Technology, Hunan Agric. Univ. Changsha 410128; 2.General Station of Forest Pest Management and Quarantine, Hunan Province Changsha 410007; 3. Institute of Animal and Plant Quarantine, CAIQ Beijing 100029

Received:2004-10-18 Revised:1900-01-01 Online:2005-03-25 Published:2005-03-25

Abstract

Abstract:

A real time fluorescent PCR(ATF-PCR) method with TaqMan probe that was designed within rDNA-ITS2 was established to detect Pine Wood Nematode, Bursaphelenchus xylophilus. The results of detecting mass DNA and single nematode showed that fluorescent signal was detected for B. xylophilus and no signal for B. mucronatus. The BXP probe was high specific for Pine Wood Nematode. The lowest concentration detected by TaqMan probe was 1 pg·μL^-1. The results of DNA sequence and ATF-PCR detection was coincidence. The research provided a new detection method for Pine Wood Nematode.

Key words: rDNA-ITS2, TaqMan probe, Bursaphelenchus xylophilus, detection

Wang Mingxu;Zhu Shuifang;Luo Kuan;Zhou Lihua;Zhao Wenjun. Studies on Real-Time Fluorescent PCR With TaqMan Probe for rDNA-ITS2 of Pine Wood Nematode(Bursaphelenchus xylophilus)[J]. Scientia Silvae Sinicae, 2005, 41(2): 82-85.

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Studies on Real-Time Fluorescent PCR With TaqMan Probe for rDNA-ITS2 of Pine Wood Nematode(Bursaphelenchus xylophilus)

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