Abstract:

Somatic embryogenesis was successfully done in Liriodendron hybrids with the immature zygotic embryos as explants. Some factors were tested about their effects on somatic embryogenesis in this research, such as genotype, developmental stage, culture condition, plant growth regulator and osmotic potential. The results showed that the embryogenic callus was successfully induced when the experimental materials were cultured on 1/2 medium supplemented with 2,4-D 2.0～3.0 mg·L^-1. Sucrose could be used as osmoticant to improve osmotic potential of the medium and it was effective for somatic embryogenesis. The induction of somatic embryos was best when the embryonic callus was cultured on MS medium supplemented with 1.0～4.0 mg·L^-1ZT. It was also proved that a proper amount of ABA could lessen the production of abnormal embryos. In addition, the genotype , developmental stage of immature embryos and light condition were important to somatic embryogenesis.

Key words: Liriodendron hybrids, Somatic embryogenesis, Embryonic callus