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Scientia Silvae Sinicae ›› 2003, Vol. 39 ›› Issue (4): 23-29.doi: 10.11707/j.1001-7488.20030404

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ISOLATION, PURIFICATION AND ANTIBODY RAISING OF RUBBER PARTICLE BINDING PROTEINS FROM EUCOMMIA ULMOIDES

Wang Minjie1,Han Yuzhen2,Liu Weiping3,Fu Yongfu5,Zhao Degang4   

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University Beijing100094;Agricultural Bioengineering Key Laboratory, Guizhou University Guiyang550025
  • Received:2001-10-10 Revised:1900-01-01 Online:2003-07-25 Published:2003-07-25

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Abstract:

Rubber particles were isolated from Eucommia ulmoides by repeated centrifuging and washing. The results of SDS-PAGE analysis showed that more than 10 proteins associated with E. ulmoides rubber particles, among which 56 ku and 30 ku proteins were more in abundance, we named them EuRPP56 and EuRPP30. The expression of rubber particle proteins was not the same in male and female. In female EuRPP30 was more abundant than EuRPP56 , while in male, EuRPP56 was more abundant than EuRPP30. EuRPP30 was prepared and purified. We raised the antibody against EuRPP30. Immunofluorecent experiments showed that the gutta_containing cells could be stained. These works laid the foundation for studying the function of the rubber particle binding proteins as well as probing into the mechanism of E. ulmoides rubber biosynthesis.

Key words: Eucommia ulmoides, Rubber particle binding protein, Antibody raising