7个桉树杂交亲本RAPD位点多态性和杂合性的研究(英文)

doi:10.11707/j.1001-7488.20030228

Scientia Silvae Sinicae ›› 2003, Vol. 39 ›› Issue (2): 162-167.doi: 10.11707/j.1001-7488.20030228

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DNA POLYMORPHISM AND HETEROZYGOSITY IN SEVEN PARENTAL EUCALYPT CLONES AS REVEALED BY RAPD MARKERS

Gan Siming,Bai Jiayu,Wu Kunming,Wu Juying,Shi Jisen

Research Institute of Tropical Forestry,Chinese Academy of Forestry Guangzhou 510520；Laboratory of Forestry Genetics and Gene Engineering,Nanjing Forestry University Nanjing210037

Received:2000-04-13 Revised:1900-01-01 Online:2003-03-25 Published:2003-03-25

Abstract

Abstract:

Five controlled crosses of Eucalyptus urophylla S.T.Blake×E.tereticornis Smith were used for RAPD analysis on DNA polymorphism and heterozygosity of the seven parental clones.A total of 58 fragments were amplified with 9 single arbitrarily chosen primers,and 42 fragments(72.4%) were scored as polymorphic among the parents.The genetic distance (GD) between parents was calculated with RAPD data matrix.High levels of heterozygosity were revealed in the parents studied,with an average 28.0%,but the percentage of heterozygous loci varied with parent from 16.2% to 39.5%.The implications for material selection in genetic linkage map construction were discussed.

Key words: RAPD, Polymorphism, Heterozygosity, Eucalypt species

Gan Siming;Bai Jiayu;Wu Kunming;Wu Juying;Shi Jisen. DNA POLYMORPHISM AND HETEROZYGOSITY IN SEVEN PARENTAL EUCALYPT CLONES AS REVEALED BY RAPD MARKERS[J]. Scientia Silvae Sinicae, 2003, 39(2): 162-167.

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DNA POLYMORPHISM AND HETEROZYGOSITY IN SEVEN PARENTAL EUCALYPT CLONES AS REVEALED BY RAPD MARKERS

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