基于培养性质模糊解析和28S rDNA-PCR-RFLP解析的外担子菌属真菌的分类学研究*

摘要/Abstract

摘要：

外担子菌属(Exobasidium)的真菌引起树木的细胞增生性和肥大性病害。本研究基于PSA培养基上菌落的形态和性状的模糊解析,以及分离菌株在不同温度、pH值、氮源、碳源下生长曲线的模糊解析和28S rDNA-PCR-RFLP解析,分布于日本的12个种和1个变种的外担子菌属真菌的35个分离菌株被分类为18个菌群。其中9个菌群和传统分类的种是一致的,这些种包括:坏损外担子菌(E.vexans Mass)、网状外担子菌(E.reticulatum Ito et Sawada)、山茶外担子菌(E.camelliae Shirai)、E.cylindrosporium Ezuka、E.shiraianum P. Heen.、E.hachijoense Otani, Kakishima et Iijima、E.bisporum Sawada et Ezuka、E.pievidis-ovalifoliae Sawada和E.symploci-japonicae Kusano et Tokubuchi;细丽外担子菌(E.gracile (Shirai) Sydow)的7个菌株分化为4个菌群,表现了种内的地理、寄主分化;日本外担子菌(E.japonicum Shirai)的7个菌株分化为5个菌群,表现了种内的寄主分化; E.yoshinagai P. Heen.和E.otanianum Ezuka var. satsumense Zhang et Arai的菌株以及E.japonicum的3个菌株被分类为1个菌群。

关键词: 外担子菌, 培养性质, RFLP, 模糊解析, 分类学

Abstract:

The fungus belonging to the genus Exobasidium (Basidiomycotina, Ustomycetes, Exobasidiales) is known as the pathogen of cell's hyperplastic and hypertrophic disease of several tree plants. In this study, thirty-five isolates (isolated from Japan) of twelve species and one variety of the genus were divided into eighteen groups based on the fuzzy analysis of cultural properties (shapes and characters of colonies; growth curve of the isolates under different temperatures, pHs, nitrogen sources and carbon sources) and the analysis of 28S rDNA-PCR-RFLP. Among the eighteen groups, nine groups were identical with the species identified by the traditional taxonomy. These species are E.vexans Mass、E.reticulatum Ito et Sawada、E.camelliae Shirai、E.cylindrosporium Ezuka、E.shiraianum P. Heen.、E.hachijoense Otani, Kakishima et Iijima、E.bisporum Sawada et Ezuka、E.pievidis-ovalifoliae Sawada and E.symploci-japonicae Kusano et Tokubuchi. Tested seven isolates of E.gracile (Shirai) Sydow were differentiated into four groups that showed the differentiation related to geographical environment and hosts in the species. Tested seven isolates of E.japonicum Shirai were differentiated into five groups that showed the differentiation related to hosts in the species. Tested isolates of E.yoshinagai P. Heen., E.otanianum Ezuka var. satsumense Zhang et Arai and the three isolates of tested seven isolates of E.japonicum were classified into one group.

Key words: Exobasidium, Cultural properties, RFLP, Fuzzy analysis, Taxonomy

张星耀. 基于培养性质模糊解析和28S rDNA-PCR-RFLP解析的外担子菌属真菌的分类学研究^*[J]. 林业科学, 1998, 34(4): 59-71.

Xingyao Zhang. A STUDY ON THE TAXONOMY OF EXOBASIDIUM SPP. ACCORDING TO THE FUZZY ANALYSIS OF CULTURAL PROPERTIES AND THE ANALYSIS OF 28S rDNA-PCR-RFLP[J]. Scientia Silvae Sinicae, 1998, 34(4): 59-71.

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参考文献 16

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