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林业科学 ›› 2009, Vol. 12 ›› Issue (5): 137-142.doi: 10.11707/j.1001-7488.20090521

• 论文 • 上一篇    下一篇

华南红树林溶磷菌16S rDNA PCR-RFLP分析及其溶磷能力*

陆俊锟1 陈 俊1,2 康丽华1 杨振德2   

  1. (1.中国林业科学研究院热带林业研究所 广州510520; 2.广西大学林学院 南宁530004)
  • 收稿日期:2008-05-12 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 康丽华

Analysis of the 16S rDNA PCR-RFLP and the Phosphate-Dissolving Capacity of Phosphate-Dissolving Bacteria Isolated from Rhizosphere of Mangrove in Southern China

Lu Junkun1,Chen Jun1,2,Kang Lihua1,Yang Zhende2   

  1. (1.Research Institute of Tropical Forestry,CAF Guangzhou 510520; 2. College of Forestry,Guangxi University Nanning 530004)
  • Received:2008-05-12 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

关键词: 红树林, 溶磷菌, RFLP, 系统发育, 多样性, 溶磷能力

Abstract:

Phosphate-dissolving bacteria isolated from rhizosphere of mangrove were characterized by 16S rDNA PCR-RFLP and 16S rDNA sequence to describe the genetic diversity and phylogeny. The dendrogram of 16S rDNA PCR-RFLP revealed the 24 isolated stains were assembled at 59% similarity level. The strains were grouped into 7 subgroups at 80% similarity. Phylogenetic tree of 16S rDNA sequences was constructed with representative strains in reference to the homogenous sequences in Genebank, and the result showed that Bacillussp. was the dominant member,besides Paenibacillus sp.,Vibrio sp.,Klebsiella sp. and Pseudomonassp. were also found.The highly genetic diversity was highly revealed by this study.In addition, the phosphate-dissolving capacity to dissolve solubilize the unsolvable inorganic phosphorus of bacteria was tested.All representative strains had the phosphate-dissolving capacity.The pH of liquid media of strains both were reduced to some degree, but the correlations were not found between phosphate-dissolving capacity and pH of the culture solution.

Key words: mangrove, phosphate-dissolving bacteria, RFLP, phylogeny, diversity, phosphate-dissolving capacity