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林业科学 ›› 2018, Vol. 54 ›› Issue (1): 54-63.doi: 10.11707/j.1001-7488.20180106

• 论文与研究报告 • 上一篇    下一篇

不同光环境下天台鹅耳枥叶形变化的测定与分析

陈模舜, 金则新, 柯世省   

  1. 浙江省植物进化生态学与保护重点实验室 台州学院生命科学学院 台州 318000
  • 收稿日期:2017-01-03 修回日期:2017-04-18 出版日期:2018-01-25 发布日期:2018-03-01
  • 基金资助:
    国家自然科学基金项目(51279121);浙江省植物进化生态学与保护重点实验室项目(1403ky05)。

Measurement and Analysis of Leaf Shape Variation of Carpinus tientaiensis in Different Light Environment

Chen Moshun, Jin Zexin, Ke Shisheng   

  1. Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation School of Life Sciences, Taizhou University Taizhou 318000
  • Received:2017-01-03 Revised:2017-04-18 Online:2018-01-25 Published:2018-03-01

摘要: [目的]天台鹅耳枥属于极少种群植物,环境适应能力较弱,仅分布于浙江省天台县和磐安县。叶片是对光环境变化敏感且可塑性较大的器官,通过几何学形态测定分析天台鹅耳枥叶形变化与环境因素之间的关系,探究不同光环境下天台鹅耳枥叶的异速生长模式,以期为天台鹅耳枥的苗木培育提供理论依据。[方法]研究天台鹅耳枥叶形对不同模拟生长环境光强(低光强LI、中等光强MI和全光照FI)的响应。3月中旬在叶未展开时对天台鹅耳枥进行不同遮荫处理,7月下旬采集成熟叶片。运用几何学形态测定法分析天台鹅耳枥3种光照梯度下叶形之间的差异,基于Tpsdig 2程序将叶片轮廓的17个地标点数字化为标准的图像,用IMP系列软件中的Coordgen软件计算每个梯度种群的标准轮廓坐标数据,用薄板样条曲线图来表示相对形态上的变化,应用PAST 3.14软件显示叶形和叶脉方面的差异。环境因素对叶形态的影响采用SPSS 11.5软件进行相关分析。[结果]通过天台鹅耳枥叶的几何学形态测定分析,表明叶片为椭圆形,先端渐钝尖,基部微心形;不同光环境下叶片形态相近,但叶形异速生长较明显。经过主成分分析和多变量方差分析,结果显示3个有意义的叶形状变量,其主成分占总方差的77.48%。相关性分析表明,天台鹅耳枥叶的形态学差异与光合有效辐射(PAR)、地表温度(Ts)、大气温度(Ta)和相对湿度(RH)显著相关(P<0.05)。当叶形变化与PAR、TsTa显著正相关、与RH显著负相关时,叶片出现中部的扩张或收缩,变异聚焦于叶基和叶尖交替的伸缩率;生长于弱光环境下,叶片中部出现扩展、叶尖压缩;在强光环境中,叶片中部挤压、叶尖膨大。当叶形变化与PAR、TsTa呈显著负相关、与RH显著正相关时,叶形变化涉及叶柄长度和叶尖伸缩率;在强光和弱光环境下,天台鹅耳枥叶柄伸长、叶尖收缩;在中等光强下,叶柄收缩、叶尖膨大。当叶形变化仅与PAR、TsTa显著正相关时,叶形变化涉及叶片的伸缩率;在中等光强下,叶柄收缩,叶片下半部挤压、叶尖膨大。利用叶形状的主成分数据作相对扭曲图验证,显示由于受光环境的影响,天台鹅耳枥叶柄和叶尖出现上、下扭曲。[结论]不同光环境下天台鹅耳枥叶存在异速生长,随着光照的增强天台鹅耳枥改变叶片形态、调节叶柄位置增加光合能力。天台鹅耳枥叶形变化与光环境的相关分析显示,与其中等光强的林窗环境相适应,天台鹅耳枥叶片椭圆形较为饱满,叶基和叶片下半部收缩,叶尖膨大,较短的叶柄能更有效地传导水分和养料。天台鹅耳枥在自然状态下依赖于特殊的生境,引种栽培天台鹅耳枥时,选择光照较强的林窗环境,可有效恢复和扩大天台鹅耳枥种群。

关键词: 天台鹅耳枥, 叶形, 几何学形态测定法, 光环境

Abstract: [Objective] Carpinus tientaiensis is a plant with extremely small population and poor acclimatization, it is only distributed in Tiantai County and Pan'an County of Zhenjiang Province. The leaf is sensitive to light conditions and with a strong plasticity. The relationship between leaf shape variability and environmental factors of C. tientaiensis was analyzed by geometric morphometrics, and the differential growth mode of the leaf of C. tientaiensis under different light environment was explored, providing a theoretical basis for cultivation of C. tientaiensis.[Method] The response of leaf shape of C. tientaiensis to irradiance intensity in varied simulated growth environment (low irradiance LI, moderate irradiance MI and full irradiance FI) was studied. In mid-March, different shading treatments were carried out on C. tientaiensis before leaf expansion, and mature leaves were collected in late July. To investigate the differences of leaf shape among 3 groups of irradiance gradients, geometric morphometrics was used, while the 17 landmark points of leaf profile were digitalized to be standard images on the basis of Tpsdig 2 program. The Coordgen software in IMP series software was used to calculate the standard contour coordinates data of each gradient population, and the thin plate spline graph was used to indicate the relative morphological changes. The application of PAST 3.14 showed differences in leaf shape and vein. The effect of environmental factors on leaf morphology was analyzed by SPSS 11.5 software.[Result] The geometric morphometrics analysis of C. tientaiensis leaf indicated that the leaf was oval, and the base was slightly heart-shaped with a gradual pointy tip. In different light conditions, the leaf shapes were similar, but the allometry of leaf shape was more obvious. Principal component analysis and multivariate analysis of variance showed three significant leaf shape variables, and the principal component of the total variance was 77.48%. Correlation analysis showed that the morphological difference of C. tientaiensis leaf was significantly correlated with photosynthetic active radiation (PAR), surface temperature (Ts), atmospheric temperature (Ta) and relative humidity (RH) (P<0.05). When the leaf shape changes had significant positive correlations with PAR, Ts and Ta, and significant negative correlation with RH, the leaves appeared to expand or shrink in the middle, and the variation focuses on the alternating expansion of the leaf blade and leaf apex. Growing in weak light environment, the middle of the leaf was expanded and the leaf apex was compressed. In strong light environment, there was extrusion of the middle of the leaves and enlargement of the leaf apex. When the leaf shape change had significant negative correlations with PAR, Ts and Ta, and significant positive correlation with RH, the petiole length and leaf apex stretch ratio were involved in the leaf shape changes. In strong light and weak light environments, the petiole was elongated and the leaf apex contracted. The petiole contracted and the leaf apex enlarged in the moderate irradiance. Only when the leaf shape changes had significant positive correlations with PAR, Ts and Ta, it involved with the leaf expansion rate. Under the moderate irradiance, the petiole shrank, the lower half of the blade was extruded, and the leaf apex was dilated. By using principal component data to make the relative distortion of leaf shape, it showed under the influence of light environment the petiole and leaf apex appeared twisted up and down.[Conclusion] In different light environments, C. tientaiensis leaves grow at allometry. With the enhancement of light, C. tientaiensis changes the shape of the leaves, it regulates the position of petioles, in order to increase its photosynthetic capacity. The correlation analysis between leaf variation of C. tientaiensis and light environment showed that due to the adaptation to the moderate irradiance in forest gap, C. tientaiensis oval leaves become more full. The basal and the lower half of the blade were extruded, while the leaf apex was dilated. Shorter petioles were more effective in conducting water and nutrients. In nature, C. tientaiensis relies on special growing environment. The introduction and cultivation of the C. tientaiensis should choose forest gaps where the light is stronger, in favor to restore and expand the C. tientaiensis population effectively.

Key words: Carpinus tientaiensis, leaf shape, geometric morphometrics, light environment

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