杨干象对12种植物挥发物的电生理及行为反应

doi:10.11707/j.1001-7488.20170619

摘要/Abstract

摘要： [目的] 从12种植物挥发物中筛选出对杨干象成虫具有显著驱避、引诱作用的挥发性物质，并为生产制备驱避引诱剂提供依据，为有效控制杨干象种群奠定基础。[方法] 利用EAG技术对12种植物挥发物质进行触角电生理反应，筛选出与对照差异显著的植物挥发物，再进行浓度梯度筛选。进而，进行Y型嗅觉仪和风洞生物测定。[结果] 糠醛、苯甲酸、丁香酚、橙花醇、香茅醇、香柏油6种植物挥发物在1mol·L^-1浓度下均能引起雌雄成虫较强的EAG反应（P<0.05）；行为反应测试表明：Y型嗅觉仪生测中1mol·L^-1寄主植物挥发物糠醛对杨干象雌虫有明显引诱作用（P<0.01），引诱率达71.59%；1mol·L^-1非寄主植物挥发物橙花醇对杨干象雌虫具有较强的驱避作用（P<0.01），驱避率高达81.5%。风洞生测中1mol·L^-1寄主植物挥发物苯甲酸对杨干象雌虫具有明显驱避作用，驱避率达74.68%。1 mol·L^-1非寄主植物挥发物香柏油对杨干象雄虫具有驱避作用（P<0.01），驱避率达78.75%。[结论] 在所选择的挥发物中，非寄主植物挥发物对杨干象成虫的行为影响略高于寄主植物；杨干象雌虫较雄虫对植物源挥发物更敏感；Y型嗅觉仪和风洞生测都获得了比较理想的试验结果。

关键词: 杨干象, 植物挥发物, 触角电位, Y型嗅觉仪, 风洞

Abstract: [Objective] To select volatile substances which could significantly repel or lure Cryptorrhynchus lapathi by screening 12 plant volatiles, and provide theoretical basis for production of repellents or attractants to effectively control this pest.[Method] EAG technique was used to exam antennal electrophysiology reactions of C. lapathi to 12 plant volatiles. Substances showed significant difference comparing with control were selected, and the effective concentrations were determined. Y type olfactometer and wind tunnel bioassays were carried out based on the results of EAG experiment.[Result] Six plant volatiles, furfural, benzoic acid, eugenol, nerol, β-cltronellol, and cedar oil, could arouse both male and female adult showing significantly higher EAG reaction (P< 0.05), and the effective concentration was 1 mol·L^-1. In Y type olfactometer behavioral response test, furfural (a host plant volatile) showed significantly higher luring effect on female adults of C. lapathi (P< 0.01), and the luring rate was 71.59%. Nerol (a non-host plant volatile) showed significantly higher repelling effect on female adults of C. lapathi (P < 0.01), and the repelling rate was up to 81.5%. In the wind tunnel bioassay, benzoic acid (a host plant volatile) showed significantly higher repelling effect on female C. lapathi, the repelling rate reached 74.68%, and male C. lapathi was significantly repelled by cedar oil (a non-host plant volatile) (P < 0.01), the repelling rate was 78.75%.[Conclusion] Non-host plant volatiles showed slightly higher impact on the behavior of adult C. lapathi than host plant volatiles, and females of C. lapathi were more sensitive to those plant volatiles than males in our tests. Both Y type olfactometer and the wind tunnel bioassays produced ideal results.

Key words: Cryptorrhynchus lapathi, plant volatiles, electroantennogram(EAG), Y type olfactometer, wind tunnel

中图分类号:

S763.306

邢亚, 迟德富, 宇佳, 严峻鑫, 冉亚丽. 杨干象对12种植物挥发物的电生理及行为反应[J]. 林业科学, 2017, 53(6): 159-167.

Xing Ya, Chi Defu, Yu Jia, Yan Junxin, Ran Yali. EAG and Behavioral Responses of Cryptorrhynchus lapathi (Coleoptera: Curculionidae) to Twelve Plant Volatiles[J]. Scientia Silvae Sinicae, 2017, 53(6): 159-167.

参考文献

曹庆杰,迟德富.2015.杨树品系抗杨干象水平及其与木质部和韧皮部硬度等关系.林业科学,51(5): 56-67.
(Cao Q J,Chi D F.2015.Relationship between the resistence level of poplar strain resist to Cryptorrhynchus lapathi(Coleoptera: Curculionidae)and the physical properties of their trunks.Scientia Silvae Sinicae,51(5): 56-67.[in Chinese])
陈琳.2014.植物挥发物与雌蛾性腺提取物对分月扇舟蛾成虫行为的影响.南昌: 江西农业大学硕士学位论文.
(Chen L.2014.Effects of plant volatiles and sex gland extracts of female moths on behavioral responses of the black-back prominent moth adults,Clostera anastomosis.Nanchang: MS thesis of Jiangxi Agricultural University.[in Chinese])
程立超.2007.十种杨树树皮挥发性物质对青杨脊虎天牛成虫的影响.哈尔滨: 东北林业大学硕士学位论文.
(Cheng L C.2007.The impact of volatile from bark of ten species of poplar to Xylotrechus rusticus L.Harbin: MS thesis of Northeast Forestry University.[in Chinese])
高瑞桐,杨自湘,陈鸿雕,等.1987.杨树品种对杨干象敏感性的初步研究.森林病虫通讯,(3): 16-18.
(Gao R T,Yan Z X,Chen H D,et al.1987.Research on sensitivity of poplar strains against Cryptorrhynchus lapathi L.Forest Pest and Disease,(3): 16-18.[in Chinese])
韩宝瑜,韩宝红.2007.无翅茶蚜对茶树挥发物的触角电生理和行为反应.生态学报,27(11): 4485-4490.
(Han B Y,Han B H.2007.EAG and behavioral responses of the wingless tea aphid Toxoptera aurantii(Homoptera: Aphididae)to tea plant volatiles.Acta Ecologica Sinica,27(11): 4485-4490.[in Chinese])
金大勇,吕龙石,李龙根,等.2003.我国杨干象研究现状及发展趋势.东北林业大学学报,31(6): 75-77.
(Jin D Y,Lü L S,Li L G,et al.2003.Status and perspective of research on Cryptorrhynchus lapathi in China.Journal of Northeast Forestry University,31(6): 75-77.[in Chinese])
李静静,黄翠虹,周琳,等.2014.风洞技术.应用昆虫学报,51(2): 591-596.
(Li J J,Huang C Y,Zhou L,et al.2014.The windtunnel technique.Journal of Applied Entomology,51(2): 591-596.[in Chinese])
李亚杰,钟兆康,林继惠,等.1981.杨干象虫的生物学和防治.昆虫学报,24(4): 390-395.
(Li Y J,Zhong Z K,Lin J H,et al.1981.Biology and control of Cryptorrhynchus lapathi L.Acta Entomologica Sinica,24(4): 390-395.[in Chinese])
李源,尹春燕,刘小侠,等.2010.多种植物挥发物及马铃薯甲虫聚集素对马铃薯甲虫的引诱作用.昆虫学报,53(7): 734-740.
(Li Y,Yin C Y,Liu X X,et al.2010.Attraction of Leptinotarsa decemlineata(Say)(Coleoptera: Chrysomelidae)by several plant volatiles and aggregation pheromone.Acta Entomologica Sinica,53(7): 734-740.[in Chinese])
苗建材,迟德富,李清宇,等.1990.氧乐果微胶囊剂的制备级防治杨干象的研究.东北林业大学学报,18(4): 35-42.
(Miao J C,Chi D F,Li Q Y,et al.1990.Preparation of omethoate microcapsulated pestcides and control Cryptorrhynchus lapathi L.Journal of Northeast Forestry University,18(4): 35-42.[in Chinese])
秦世剑,沈明日.2010.杨干象的发生规律及综合治理技术.林业科技情报,42(1): 20-21.
(Qin S J,Shen M R.2010.Occurrence regulation and integrated management techniques of Cryptorrhynchus lapathi. Journal of Forestry Science and Technology Information,42(1): 20-21.[in Chinese])
宋子英,张海.2006.林业有害生物杨干象危险性分析.甘肃林业科技,31(1): 51-53.
(Song Z Y,Zhang H.2006.Forest pest risk analysis of Cryptorrhynchus lapathi.Journal of Gansu Forestry Science and Technology,31(1): 51-53.[in Chinese])
王振华,赵晖,李金甫,等.2008.植物源挥发物对昆虫信息素的增效作用及其增效机制.应用生态学报, 19(11): 2533-2537.
(Wang Z H,Zhao H,Li J P,et al.2008.Synergism of plant volatiles to insects pheromones and related mechanisms.Chinese Journal of Applied Ecology, 19(11): 2533-2537.[in Chinese])
严善春,刘英胜,王琪,等.2007.落叶松毛虫对兴安落叶松6种挥发物的触角电位反应.林业科学,43(7): 55-60.
(Yan S C,Liu Y S,Wang Q, et al.2007.Electroantennogram responses of Dendrolimus superans to six volatiles of Larix gmelinii.Scientia Silvae Sinicae,43(7): 55-60.[in Chinese])
余玉婷.2013.植物挥发物对金龟甲的引诱作用研究.南昌: 江西农业大学硕士学位论文.
(Yu Y T.2013.Attraction of scarab beetles to plant volatiles.Nanchang: MS thesis of Jiangxi Agricultural University.[in Chinese])
张振,迟德富,宇佳,等.2010.青杨脊虎天牛对13种植物挥发物的电生理及行为反应.林业科学,46(10): 69-75.
(Zhang Z,Chi D F,Yu J, et al.2010.EAG behavioral responses of Xylotrechus rusticus to thirteen plant volatiles.Scientia Silvae Sinicae,46(10): 69-75.[in Chinese])
Anderson A R,Wanner K W,Trowell S C,et al.2009.Molecular basis of female-specific odorant responses in Bombyx mori.Insect Biochemistry and Molecular Biology,39: 189-197.
Cao Q J,Yu J, Chi D F,et al.2015.Effects of plant volatiles on electrophysiological and behavioral responses of Cryptorrhynchus lapathi.Entomologia Experimentalis et Applicata,156(2): 105-116.
Flint H M,Salter S S,Walters S.1979.Caryophyllene: an attractant for the green lacewing. Environ Entomol,8(6): 1123-1125.
Leal W S,Uchida K.1998.Application of GC-EAD to the determination of mosquito repellents derived from a plant,Cymbopogon citratus.Journal of Asia-Pacific Entomol,1(2): 217-221.
Pickett J A,Wadhams L J,Woodcock C M, et al. 1992.The chemical ecology of aphids.Annu Rev Entomol,37: 67-90.
Raguso R A,Light D M, Pickersky E.1996.Electroantennogram responses of Hyles lineata(Sphingidae: Lepidoptera) to volatile compounds from Clarkia breweri(Onagraceae) and other moth-pollinated flowers.Journal of Chemical Ecology,22(10): 1735-1766.
Raguraman S,Singh D.1997.Biopotential of Azadirachta indica and Cedrus deodara oils on Callosobruchus chinensis L.Journal of Pharmaceutical Biology,35(5): 344-348.
Reddy G A P,Guerrero A.2004.Interactions of insect pheromones and plant semiochemicals.Trends in Plant Science,9(5): 253-261.
SOLÉ J,SANS A,RIBA M,et al.2010.Behavioural and electrophysiological responses of the European corn borer Ostrinia nubilalis to host-plant volatiles and related chemicals.Journal of Pharmaceutical Biology,35(4): 354-363.
Xu H X,Wang S,Tan X L,et al.2011.Electrophysiological responses of Plutella xylostella to 9 different plantvolatiles.Journal of Environmental Entomology,33(4): 442-447.
Zhang Z Q,Sun X L,Luo Z X,et al.2013.The manipulation mechanism of "push-pull" habitat management strategy and advances in its application.Acta Ecologica Sinica, 33(2): 94-101.

[1]	付宁宁, 刘佳, 渠成, 王然, 许奕华, 罗晨, 李峰奇. 悬铃木方翅网蝽化学感受蛋白CcilCSP1的结构及其结合寄主挥发物的预测分析[J]. 林业科学, 2017, 53(10): 109-117.
[2]	曹庆杰, 迟德富. 感染杨干象幼虫的高致病力虫生真菌菌株筛选[J]. 林业科学, 2017, 53(1): 119-127.
[3]	陈大风, 李叶静, 孔祥波, 张真, 王鸿斌, 焦相杰, 段瑞龙, 陈洁, 李继峰. 落叶松八齿小蠹对聚集信息素和寄主挥发性萜烯成分的电生理响应[J]. 林业科学, 2013, 49(11): 89-97.
[4]	杨美红;张金桐;宗世祥;骆有庆;曹川健;范丽华;刘红霞;辛海萍. 榆木蠹蛾性诱剂的合成及林间诱蛾试验[J]. 林业科学, 2012, 48(4): 61-66.
[5]	杨文斌;王晶莹;董慧龙;王林和;张国盛;卢琦;赵爱国. 两行一带式乔木固沙林带风速流场和防风效果风洞试验[J]. 林业科学, 2011, 47(2): 95-102.
[6]	杨立军;李新岗. 松果梢斑螟对油松球果挥发物的触角电位反应[J]. 林业科学, 2011, 47(1): 172-176.
[7]	程彬;付晓霞;韩启;张宝民;张大明;李兴鹏;高长启;孙晓玲. 虫害诱导的家榆挥发物对榆紫叶甲寄主选择行为的影响[J]. 林业科学, 2010, 46(10): 76-82.
[8]	张振;迟德富;宇佳;李晓灿;赵晓杰. 青杨脊虎天牛对13种植物挥发物的电生理及行为反应[J]. 林业科学, 2010, 46(10): 69-75.
[9]	严善春杨慧高璐璐王志波毛洪波. 兴安落叶松鞘蛾对寄主挥发物的反应[J]. 林业科学, 2009, 12(5): 94-101.
[10]	严善春刘英胜王琪程红张健迟德富. 落叶松毛虫对兴安落叶松6种挥发物的触角电位反应[J]. 林业科学, 2007, 43(07): 55-60.
[11]	张同心;孙绪艮崔为正梁中贵;. 松阿扁叶蜂对不同树种的选择行为[J]. 林业科学, 2006, 42(06): 66-70.
[12]	李新岗刘惠霞刘拉平马养民. 影响松果梢斑螟寄主选择的植物挥发物成分研究[J]. 林业科学, 2006, 42(06): 71-78.
[13]	王鸿斌;王玉刚张真金幼菊周淑芷. 油松挥发性物质分析及红脂大小蠹的触角电位反应[J]. 林业科学, 2006, 42(05): 84-88.
[14]	邓湘雯文定元申初联何介南刘豪健唐刚. 防火林带迎风面风速分布规律的风洞试验[J]. 林业科学, 2005, 41(6): 114-118.
[15]	张金桐孟宪佐. 小木蠹蛾雄蛾对雌蛾性信息素提取物及合成标准化合物的电生理反应[J]. 林业科学, 2000, 36(5): 123-126.