干旱胁迫下马尾松幼苗针叶挥发性物质与内源激素的变化

doi:10.11707/j.1001-7488.20170406

摘要/Abstract

摘要： [目的] 探讨干旱胁迫下马尾松幼苗针叶内源激素含量变化与挥发性物质释放规律，为马尾松抗逆生理研究和育苗提供科学依据。[方法] 采用顶空固相微萃取（HS-SPME）结合气相色谱-质谱联用技术（GC-MS），对不同干旱胁迫处理的马尾松幼苗针叶挥发性物质进行鉴定，并采用高效液相色谱串联三重四级杆质谱法（LC-MS/MS）测定其内源激素含量。[结果] GC-MS鉴定出13种挥发性物质，属于萜类、醛类、醇类和酯类化合物，其中9个化合物确定为萜类，相对含量较高的物质是α-蒎烯、石竹烯和α-水芹烯。干旱胁迫时马尾松幼苗针叶单萜类相对含量先降低后逐渐升高，倍半萜类相对含量则先升高再逐渐降低，干旱胁迫明显诱导针叶单萜类含量增加；马尾松幼苗针叶脱落酸（ABA）和玉米素（ZT）含量随着干旱胁迫程度加大呈明显增加趋势，赤霉素（GA）和生长素（IAA）含量随着干旱胁迫程度加大呈逐渐降低趋势。[结论] HS-SPME可作为干旱胁迫下马尾松幼苗针叶挥发性物质提取的有效手段，GC-MS适合于马尾松幼苗针叶挥发性物质的识别；干旱胁迫时马尾松幼苗针叶含有丰富的单萜和倍半萜，针叶通过调节单萜与倍半萜比例响应干旱胁迫；干旱胁迫时马尾松幼苗针叶通过内源激素相互协调来适应干旱逆境，其中ZT和ABA是其重要的干旱胁迫信号激素。

关键词: 马尾松, 干旱胁迫, 挥发性物质, 内源激素

Abstract: [Objective] This study aimed to reveal the change pattern of endogenous hormones content and volatile compounds in Masson pine seedlings under drought stress, and to provide a foundation for studying the stress-resistance physiology and nursery of Masson pine.[Method] The seedling volatiles, under different drought stress, were identified by headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), and the endogenous hormones were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).[Result] A total of 13 types of volatiles were identified with GC-MS, they belong to terpenoids, aldehydes, alcohols, and ester compounds. Nine types were identified as terpenoids, and among them, α-pinene, caryophyllene, and α-phellandrene had relatively higher content. Under drought stress, relative content of monoterpenes decreased first and then increased as the drought stress intensified, but relative content of sesquiterpenes increased first and then decreased. Drought conditions promoted the synthesis of monoterpenes. The content of ABA and ZT in needles was gradually increased with the strengthening of drought stress, but GA and IAA content was decreased gradually with the strengthening of drought stress.[Conclusion] HS-SPME could be one of the effective techniques to extract the volatiles of Masson pine under drought stress condition, and GC-MS is suitable for the needle volatiles identification. Under drought stress, Masson pine needles are rich in monoterpenes and sesquiterpenes. Through altering the ratio of monoterpenes and sesquiterpenes, seedlings enhanced the drought resistance. Endogenous hormones improved the drought-resistant ability to adapt the drought stress through coordination with each other. ZT and ABA are important hormones of drought stress, and deserve further study.

Key words: Pinus massoniana, drought stress, volatiles, endogenous hormones

中图分类号:

S718.43

全文选, 丁贵杰. 干旱胁迫下马尾松幼苗针叶挥发性物质与内源激素的变化[J]. 林业科学, 2017, 53(4): 49-55.

Quan Wenxuan, Ding Guijie. Dynamic of Volatiles and Endogenous Hormones in Pinus massoniana Needles under Drought Stress[J]. Scientia Silvae Sinicae, 2017, 53(4): 49-55.

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