可溶性有机物输入对亚热带森林土壤CO2排放及微生物群落的影响

doi:10.11707/j.1001-7488.20160213

摘要/Abstract

摘要： [目的] 研究可溶性有机物(DOM)输入对森林土壤CO₂排放及微生物群落的影响,为探讨DOM在森林生态系统碳循环中的作用提供依据。[方法] 设置添加米槠凋落叶DOM、杉木凋落叶DOM、米槠枯死根DOM、杉木枯死根DOM及添加去离子水(对照)处理,通过36 h短期室内培养,研究添加米槠及杉木凋落叶和枯死根DOM后土壤CO₂排放速率最大值出现的时间及对土壤微生物群落的影响。[结果] 无论米槠还是杉木,其凋落叶DOC含量均显著高于枯死根DOC含量,而凋落叶DOM的腐殖化指数(HIX)则显著低于枯死根DOM的HIX,添加米槠枯死根DOM和杉木枯死根DOM的土壤CO₂排放速率在第2 h达到最大值,分别是对照的7.3和8.3倍,在24 h时则降低至最大值的78.9%和66.3%;而添加米槠凋落叶DOM和杉木凋落叶DOM的土壤CO₂排放速率在12 h时达到最大值,分别是对照的20.6和13.2倍,在24 h时则分别降低至最大值的84.0%和53.1%;磷脂脂肪酸(PLFA)分析结果显示,土壤添加米槠凋落叶DOM后革兰氏阳性细菌、革兰氏阴性细菌、放线菌和真菌的PLFAs含量显著低于土壤添加杉木凋落叶DOM的27%,38%,46%和41% (P<0.05);土壤添加米槠枯死根DOM后革兰氏阳性细菌、革兰氏阴性细菌和真菌PLFAs含量显著低于添加杉木枯死根DOM的21%,21%和22% (P<0.05);培养36 h时,添加米槠凋落叶DOM的土壤和对照土壤中G+:G-(革兰氏阳性细菌:革兰氏阴性细菌)高于培养前,但添加米槠凋落叶DOM的土壤中真菌:细菌低于培养前,这与其他处理的结果相反,表明添加不同来源DOM对土壤微生物群落的影响不一致。[结论] 外源添加DOM后土壤CO₂排放速率最大值的出现时间由外源添加DOM的组成和化学性质决定,而且外源添加DOM显著影响土壤微生物的群落组成。

关键词: 米槠, 杉木, CO₂排放, 可溶性有机物, 微生物群落

Abstract: [Objective] DOM (dissolved organic matter) is an important labile carbon source in soil, and can be an important factor regulating CO₂ emission of forest soil. This study will improve understanding of the role of DOM on forest C cycle.[Method] We added DOM from leaf litter and dead roots of Cunninghamia lanceolata and Castanopsis carlesii to soil to examine the effects of carbon inputs on soil CO₂ efflux and microbial community composition by phospholipid fatty acid (PLFA) analysis through laboratory incubations for 36 hours. The treatments were as follows:soil with DOM from C. carlesii leaf litter, soil with DOM from C. lanceolata leaf litter, soil with DOM from C. carlesii dead root, soil with DOM from C. lanceolata dead root, and a control (soil with deionized water). Mineral soil (0-10 cm) was from an 11-year-old C. lanceolata plantation in Sanming of Fujian Province, China. Carbon mineralization was determined using CO₂respiration method.[Result] The contents of dissolved organic carbon (DOC) from leaf litter were much higher than those from dead roots, and the humification index (HIX) values of the DOM were opposite. The maximum rates of C mineralization occurred in 2 hours following addition of DOM from dead roots of C. lanceolata and C. carlesii, and were 7.3 and 8.3 times higher than that of control respectively, then decreased to 78.9% and 66.3% of the maximum values by 24 hours. In contrast, the maximum rates of C mineralization were in 12 hours following addition of DOM from leaf litter of C. lanceolata and C. carlesii, and the magnitudes were 20.6 and 13.2 times that of control respectively, then decreased to 84.0% and 53.1% of the maximum by 24 hours. PLFA analysis showed that the contents of gram-positive bacteria, gram-negative bacteria, actinomycetes and fungi in soils added with DOM from C. carlesii leaf litter were 27%, 38%, 46% and 41% lower than those of soils added with DOM from C. lanceolata leaf litter, respectively (P<0.05). Compared to soils added with DOM from dead roots of C. lanceolata, the contents of gram-positive bacteria, gram-negative bacteria and fungi were 21%, 21% and 22% lower in soils added with DOM from dead roots of C. carlesii, respectively (P<0.05). After 36 h incubation, the ratios of gram-positive bacteria to gram-negative bacteria in soils added with DOM from C. carlesii leaf litter and the control were higher than those in untreated soil, while compared to untreated soil, the ratio of fungi to bacteria was lower following additions of DOM from leaf litter of C. carlesii.[Conclusion] There was significant difference in the microbial community composition following additions of DOM from various sources, and the maximum rates of C mineralization following addition of DOM depended on the quantity and quality of DOM.

Key words: Castanopsis carlesii, Cunninghamia lanceolata, CO₂ emission, dissolved organic matter, microbial community composition

中图分类号:

S718.5

万菁娟, 郭剑芬, 纪淑蓉, 任卫岭, 杨玉盛. 可溶性有机物输入对亚热带森林土壤CO₂排放及微生物群落的影响[J]. 林业科学, 2016, 52(2): 106-113.

Wan Jingjuan, Guo Jianfen, Ji Shurong, Ren Weiling, Yang Yusheng. Effects of Dissolved Organic Matter Input on Soil CO₂ Emission and Microbial Community Composition in a Subtropical Forest[J]. Scientia Silvae Sinicae, 2016, 52(2): 106-113.

参考文献

程东升. 1993. 森林微生物生态学. 哈尔滨:东北林业大学出版社, 81-84.
(Cheng D S.1993. Forest microbial Ecology. Harbin:Northeast Forestry University Press, 81-84.[in Chinese])
万菁娟,郭剑芬,纪淑蓉,等. 2015. 杉木和米槠凋落叶DOM对土壤碳矿化的影响. 生态学报, 35(24):8148-8154.
(Wan J J, Guo J F, Ji S R, et al. 2015. Effects of dissolved organic matter from Cunninghamia lanceolata and Castanopsis carlesii leaf litter on soil C mineralization. Acta Ecologica Sinica, 35(24):8148-8154.[in Chinese])
熊丽, 杨玉盛, 王巧珍. 等. 2014. 可溶性有机碳在米槠天然林土壤中的淋溶特征. 亚热带资源与环境学报, 9(1):46-52.
(Xiong L, Yang Y S, Wang Q Z, et al. 2014. Movement of dissolved organic carbon in natural forest soil of Castanopsis carlesii. Journal of Subtropical Resources and Environment, 9(1):46-52.[in Chinese])
Ayres E, Steltzer H, Simmons B L, et al. 2009. Soil biota accelerate decomposition in high-elevation forests by specializing in the breakdown of litter produced by the plant species above them. Journal of Ecology, 97(5):901-912.
Blagodatskaya E, Kuzyakov Y. 2008. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure:critical review. Biology and Fertility of Soils, 45(2):115-131.
Boddy E, Hill P W. 2007. Fast turnover of low molecular weight components of the dissolved organic carbon pool of temperate grassland field soils. Soil Biology and Biochemistry, 39(4):827-835.
Chen Q H, Feng Y, Zhang Y P, et al. 2012. Short-term responses of nitrogen mineralization and microbial community to moisture regimes in greehouse vegetable soils. Pedosphere, 22 (2):263-272.
Cleveland C C, Neff J C, Townsend A R, et al. 2004. Composition, dynamics, and fate of leached dissolved organic matter in terrestrial ecosystems:results from a decomposition experiment. Ecosystems, 7(3):175-285.
Cleveland C C, Nemergut D R, Schmidt S K, et al. 2007. Increases in soil respiration following labile carbon additions linked to rapid shifts in soil microbial community composition. Biogeochemistry, 82(3):229-240.
Cleveland C C, Wieder W R, Reed S C, et al. 2010. Experimental drought in a tropical rain forest increases soil carbon dioxide losses to the atmosphere. Ecology, 91(8):2313-2323.
Crow S E, Lajtha K, Filley T R, et al. 2009. Sources of plant-derived carbon and stability of organic matter in soil:implications for global change. Global Change Biology, 15(8):2003-2019.
Denef K, Roobroeck D, Manimel Wadu M C, et al. 2009. Microbial community composition and rhizodeposit-carbon assimilation in differently managed temperate grassland soils. Soil Biology and Biochemistry, 41(1):144-153.
Dungait J A J, Kemmitt S J, Michallon L, et al. 2011. Variable responses of the soil microbial biomass to trace concentrations of ¹³C-labelled glucose, using ¹³C-PLFA analysis. European Journal of Soil Science, 62(1):117-126.
Feng W T, Zou X M, Schaefer D. 2009. Above- and belowground carbon inputs affect seasonal variations of soil microbial biomass in a subtropical monsoon forest of southwest China. Soil Biology and Biochemistry, 41(5):978-983.
Fontaine S, Mariotti A, Abbadie L. 2003. The priming effect of organic matter:a question of microbial competition? Soil Biology and Biochemistry, 35 (6):837-843.
Frostegrd , Tunlid A, Bth E. 2011. Use and misuse of PLFA measurements in soils. Soil Biology and Biochemistry, 43(8):1621-1625.
Garcia-Pausas J, Paterson E. 2011. Microbial community abundance and structure are determinants of soil organic matter mineralisation in the presence of labile carbon. Soil Biology and Biochemistry, 43(8):1705-1713.
Grdens A I, gren G I, Bird J A, et al. 2011. Knowledge gaps in soil carbon and nitrogen interactions -from molecular to global scale. Soil Biology and Biochemistry, 43 (4):702-717.
Housman D C, Powers H H, Collins A D, et al. 2006. Carbon and nitrogen fixation differ between successional stages of biological soil crusts in the Colorado Plateau and Chihuahuan Desert. Journal of Arid Environments, 66(4):620-634.
Kramer C, Gleixner G. 2006. Variable use of plant- and soil-derived carbon by microorganisms in agricultural soils. Soil Biology and Biochemistry, 38(11):3267-3278.
Kuzyakov Y. 2010. Priming effects:interactions between living and dead organic matter. Soil Biology and Biochemistry, 42(9):1363-1371.
Kuzyakov Y, Friedel J K, Stahr K. 2000. Review of mechanisms and quantification of priming effects. Soil Biology and Biochemistry, 32(11):1485-1498.
Landesman W J, Dighton J. 2010. Response of soil microbial communities and the production of plant-available nitrogen to a two-year rainfall manipulation in the New Jersey Pinelands. Soil Biology and Biochemistry, 42(10):1751-1758.
Leff J W, Nemergut D R, Grandy A S, et al. 2012. The effects of soil bacterial community structure on decomposition in a tropical rain forest. Ecosystems, 15(2):284-298.
Lejon D P, Chaussod R, Ranger J, et al. 2005. Microbial community structure and density under different tree species in an acid forest soil (Morvan, France). Microbial Ecology, 50(4):614-625.
Moore-Kucera J, Dick R P. 2008. Application of ¹³C-labeled litter and root materials for in situ decomposition studies using phospholipid fatty acids. Soil Biology and Biochemistry, 40(10):2485-2493.
Neff J C, Asner G P. 2001. Dissolved organic carbon in terrestrial ecosystems:synthesis and a model. Ecosystems, 4(1):29-48.
Nemergut D R, Cleveland C C, Wieder W R, et al. 2010. Plot-scale manipulations of organic matter inputs to soils correlate with shifts in microbial community composition in a lowland tropical rain forest. Soil Biology and Biochemistry, 42(12):2153-2160.
Nottingham A T, Griffiths H, Chamberlain P M, et al. 2009. Soil priming by sugar and leaf-litter substrates:a link to microbial groups. Applied Soil Ecology, 42(3):183-190.
Paterson E. 2009. Comments on the regulatory gate hypothesis and implications for C-cycling in soil. Soil Biology and Biochemistry, 41(6):1352-1354.
Paterson E, Gebbing T, Abel C, et al. 2007. Rhizodeposition shapes rhizosphere microbial community structure in organic soil. New Phytologist, 173(3):600-610.
Potts D L, Huxman T E, Cable J M, et al. 2006. Antecedent moisture and seasonal precipitation influence the response of canopy-scale carbon and water exchange to rainfall pulses in a semi-arid grassland. New Phytologist, 170(4):849-860.
Swallow M, Quideau S, MacKenzie M, et al. 2009. Microbial community structure and function:the effect of silvicultural burning and topographic variability in northern Alberta. Soil Biology and Biochemistry, 41(4):770-777.
Tavi N M, Martikainen P J, Lokko K, et al. 2013. Linking microbial community structure and allocation of plant-derived carbon in an organic agricultural soil using ¹³CO₂ pulse-chase labelling combined with ¹³C-PLFA profiling. Soil Biology and Biochemistry, 58(3):207-215.
Von Lützow M, K gel-Knabner I, Ekschmitt K, et al. 2007. SOM fractionation methods:relevance to functional pools and to stabilization mechanisms. Soil Biology and Biochemistry, 39(9):2183-2207.
Wang Q, He T, Wang S, et al. 2013. Carbon input manipulation affects soil respiration and microbial community composition in a subtropical coniferous forest. Agricultural and Forest Meteorology, 178-179:152-160.
Wieder WR, Cleveland C, Townsend A R. 2008. Tropical tree species composition affects the oxidation of dissolved organic matter from litter. Biogeochemistry, 88(2):127-138.

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可溶性有机物输入对亚热带森林土壤CO₂排放及微生物群落的影响

Effects of Dissolved Organic Matter Input on Soil CO₂ Emission and Microbial Community Composition in a Subtropical Forest

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