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林业科学 ›› 2014, Vol. 50 ›› Issue (5): 147-153.doi: 10.11707/j.1001-7488.20140519

• 研究简报 • 上一篇    下一篇

油松成熟胚培养不定芽状态对其生根状况的影响

买凯乐1, 邵砾群2, 李荣珍1, 董丽芬2, 兰健花1   

  1. 1. 广西生态工程职业技术学院 柳州 545004;
    2. 西北农林科技大学 杨凌 712100
  • 收稿日期:2013-08-28 修回日期:2014-01-02 出版日期:2014-05-25 发布日期:2014-06-06
  • 基金资助:

    国家林业局项目“干旱地区主要造林树种快速育苗及设备研究”(96—122);广西生态工程职业技术学院学院立项课题(201105A);广西高校科学技术研究项目(2013LX199)。

Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting

Mai Kaile1, Shao Liqun2, Li Rongzhen1, Dong Lifen2, Lan Jianhua1   

  1. 1. Guangxi Eco-Engineering Vocational and Technical College Liuzhou 545004;
    2. Northwest A&F University Yangling 712100
  • Received:2013-08-28 Revised:2014-01-02 Online:2014-05-25 Published:2014-06-06
  • Contact: 董丽芬

关键词: 油松, 成熟胚, 改良1/2MS培养基, 不定芽诱导, 不定芽生根

Abstract:

The mature embryo of Pinus tabulaeformis was used as explants to induce adventitious buds and root on modified 1/2MS medium. The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces. The rooting rate of adventitious buds was 91.7%. The adventitious bud clusters with diameter larger than 0.9 cm, and the shoots with length longer than 6 mm had higher rooting rate. In the process of shoot elongation and multiplication, short culture duration could lead to a higher rooting capacity of the adventitious bud cluster, and the 28-days culture period was optimal for rooting, leading to a rooting rate of 73.3%. Adventitious bud clusters with big biomass were easily to root, and the higher the rooting rate the bigger the bud cluster biomass.

Key words: Pinus tabulaeformis, mature embryo, modified 1/2MS medium, adventitious bud induction, adventitious bud rooting

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