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林业科学 ›› 2012, Vol. 48 ›› Issue (2): 63-68.doi: 10.11707/j.1001-7488.20120209

• 论文 • 上一篇    下一篇

同源四倍体刺槐花序变异表型观察与分析

路超1, 崔彬彬1,2, 黄禄君1, 孙鹏1, 张国君1,3, 李云1   

  1. 1. 林木育种国家工程实验室 北京林业大学生物科学与技术学院 北京 100083;2. 保定学院生化系 保定 071000;3. 河北科技师范学院园艺科技学院 秦皇岛 066004
  • 收稿日期:2011-06-09 修回日期:2011-08-25 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 李云

Phenotypic Observation and Analysis of Inflorescence Variation of Autotetraploid Robinia pseudoacacia

Lu Chao1, Cui Binbin1,2, Huang Lujun1, Sun Peng1, Zhang Guojun1,3, Li Yun1   

  1. 1. National Engineering Laboratory for Tree Breeding College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing 100083;2. Department of Biology and Chemistry, Baoding University Baoding 071000;3. College of Horticulture Science and Technology, Hebei Normal University of Science & Technology Qinhuangdao 066004
  • Received:2011-06-09 Revised:2011-08-25 Online:2012-02-25 Published:2012-02-25

摘要:

为揭示同源四倍体花序的遗传变异机制,对同源四倍体刺槐和二倍体刺槐的花序生长进行对比研究。结果显示:与二倍体刺槐相比,同源四倍体刺槐的花序长度没有明显变化,但着生的小花数量明显提高; 同源四倍体刺槐花序不同位置小花的发育呈现不同步性; 分别从同源四倍体刺槐与二倍体刺槐中克隆出花分生组织决定基因 LFY 和 TFL 1 的同源基因的部分片段,其同源性分别达到98.44%和98.00%,而去甲基化剂5-acazC能够明显抑制变异花序的形成和促进花序提前开花。

关键词: 刺槐, 同源四倍体, 花序变异, 甲基化, 花分生组织决定基因

Abstract:

A comparative study between autotetraploid and diploid Robinia pseudoacacia was carried out to investigate the mechanism of genetic variations of inflorescence in autotetraploid. The result showed there were no significantly differences in the length of inflorescence between autotetraploid and diploid R. pseudoacacia, but the number of florets was significantly more in autotetraploid than the diploid. The florets of the autotetraploid R. pseudoacacia in different positions showed the remarkable asynchrony. DNA sequences of homologous genes of LFY and TFL 1 were cloned from autotetraploid and diploid, and their homology reached to 98.44% and 98.00%, respectively. The treatment with the acazC could observably reduce the inflorescence variation and promote inflorescence bloom prematurely.

Key words: Robinia pseudoacacia, autotetraploid, variation of inflorescence, methylation, floral meristem identity genes

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