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林业科学 ›› 2007, Vol. 43 ›› Issue (03): 138-142.doi: 10.11707/j.1001-7488.20070324

• 研究简报 • 上一篇    

抗生素对泡桐丛枝病植原体和发病相关蛋白质的影响

范国强 张胜 翟晓巧 刘飞 董占强   

  1. 河南农业大学泡桐研究所,郑州450002
  • 收稿日期:2006-09-29 修回日期:1900-01-01 出版日期:2007-03-25 发布日期:2007-03-25

Effects of Antibiotics on the Paulownia Witches' Broom Phytoplasmas and Pathogenic Protein Related to Witches' Broom Symptom

Fan Guoqiang,Zhang Sheng,Zhai Xiaoqiao,Liu Fei,Dong Zhanqiang   

  1. Institute of Paulownia, Henan Agricultutral University Zhengzhou 450002
  • Received:2006-09-29 Revised:1900-01-01 Online:2007-03-25 Published:2007-03-25

关键词: 泡桐, 丛枝病, 蛋白质, 双向电泳, PCR

Abstract:

The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulownia tomentosa×Paulownia fortunei seedling and the pathogenic protein related to the witches' broom symptom were studied with PCR based on 16S rRNA gene of the phytoplasmas and 2-dimensional electrophoresis of proteins. The results indicated that the plantlets in vitro culture treated with 0.5~300 mg·L-1 5-fluorouracil had little effect on the symptom remission, but those plantlets treated with 100 and 150 mg·L-1 rifampicin for 30 d showed obvious effect on the symptom remission respectively, and the phytoplasmas in the plantlets treated with 150 mg·L-1 rifampicin could not be detected with PCR of 16S rDNA of the phytoplasmas. Moreover, the protein (pI6.8,m 24 ku) which appeared in both the healthy seedling and diseases ones treated with 150 mg·L-1 rifampicin disappeared in the infected seedling and the those without the symptom after treating with rifampicin and tetracycline, and in which existed with phytoplasma through PCR detection.

Key words: Paulownia;witches&rsquo, broom;protein;2-dimensional electrophoresis;PCR