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林业科学 ›› 2000, Vol. 36 ›› Issue (6): 82-85.doi: 10.11707/j.1001-7488.20000620

• 论文及研究报告 • 上一篇    下一篇

桑粒肩天牛3种纤维素消化酶的分布

殷幼平 曹月青 何正波 董亚敏   

  1. 西南农业大学 重庆 400716
  • 收稿日期:1999-11-09 修回日期:1900-01-01 出版日期:2000-11-25 发布日期:2000-11-25

ORIGIN AND DISTRIBUTION OF THREE DIGESTIVE ENZYMES IN THE BROWN MULBERRY LONGHORN BORER APRIONA GERMARI (HOPE),(COLEOPTERA:CERAMBYCIDAE)

Yin Youping,Cao Yueqing,He Zhengbo,Dong Yamin   

  1. Center of Biotechnology Southwest Argricultural University Chongqing 400716
  • Received:1999-11-09 Revised:1900-01-01 Online:2000-11-25 Published:2000-11-25

摘要: 将桑粒肩天牛唾腺匀浆、肠道分段冰冻切片后抽提,测定各部分内切β-1,4-葡聚糖酶(Cx),纤维二糖水解酶(CHB),β-1 ,4-葡萄糖苷酶在肠道及唾腺中的酶活与分布。结果发现97.13%的CX酶,95.7%的β-1,4-葡萄糖苷酶的97.03%的CHB酶存在于桑粒肩天牛中肠,唾腺中没有检测到酶活性,结合天牛消化道形态和组织学特征,证明桑粒肩天牛纤维素酶的主要来源是内源性的。

关键词: 桑粒肩天牛, 纤维素酶, 分布, 起源

Abstract: The distribution of the cellulolytic enzymes namely Endo-β-1,4-glucanase (CX),cellobiohydrolase (CBH) and β-1,4-glucosidase activities in gut and salivery glands of Apriona germari (Hope) was measured.97.13% CX,95.7% β-1,4-glucosidase and 97.03% CBH activities were found in the midgut and not detected in salivary glands.This showed that the major origin of cellulases were endogeneous.

Key words: Apriona germari (Hope), Cellulase, Distribution, Origin