枣尺蠖核多角体病毒活性及林间防治试验

林业科学 ›› 1999, Vol. 35 ›› Issue (6): 81-85.

枣尺蠖核多角体病毒活性及林间防治试验

冀卫荣,刘贤谦,师光禄

山西农业大学太谷 030801

收稿日期:1998-11-09 出版日期:1999-11-25 发布日期:1999-11-25
基金资助:
山西省科技攻关项目(972086)

THE BIOASSAY AND FIELD EFFICACY TESTS OF THE NUCLEAR POLYHEDROSIS VIRUS OF Chihuo zao Yang

Weirong Ji,Xianqian Liu,Guanglu Shi

Forestry Department, Shanxi Agricultural University Taigu 030801

Received:1998-11-09 Online:1999-11-25 Published:1999-11-25

摘要/Abstract

摘要：

本文报道了枣尺蠖(Chihuo zao Yang)核多角体病毒(CzN PV)活性的生物测定结果及在山西太谷枣树林间防治枣尺蠖的试验效果。以不同浓度该病毒悬液浸渍鲜枣树叶,饲喂感染3龄初枣尺蠖幼虫,病毒浓度对数值与死亡机率的回归直线方程为; LC₅₀及95 %的置信限分别为3.65 ×10⁵及4.04 × 10⁴ ~ 3.13 ×10⁶PI Bs/ mL。以2.5 ×10⁵、1.25×10⁶、2.5 ×10⁶、1.25 ×10⁷和2.5 ×10⁷PIBs/mL 5种浓度病毒感染3龄初幼虫,其LT₅₀值分别为10.01、9.57、9.08、7.94和7.49 d。同一浓度病毒(2.5 ×10⁶ PI Bs/ mL)对不同虫龄感染力不同。幼虫死亡率随虫龄增大而降低,从2龄的96.7%降低到4龄的30.0 %; LT₅₀值随着虫龄增大而增大,从2龄的5.97 d增加到4龄的12.57 d。在山西太谷枣林枣尺蠖幼虫2龄、3龄占85 %时喷洒浓度为2.5 ×10⁷P IBs/ mL的病毒悬液,虫口减退率高,成本较低,可在生产上推广应用。

关键词: 枣尺蠖, 核多角体病毒, 生物活性测定, 林间防治试验

Abstract:

The results of bioassay and field efficacy tests of the nuclear polyhedrosis virus of Chihuo zao Yang were reported in this paper.Early third-instar larvae were fed on jujube tree leaves immersed with polyhedral inclusion bodies(PIB)suspension of virus.Mortality of larvae increased with the increasing of concentration of polyhedral inclusion bodies suspension.The LC₅₀ and 95% confidence limits were 3.65 × 10⁵ and 4.04 ×10⁴ ~ 3.13 ×10⁶PIBs/mL, respectively.The LT₅₀ values for the virus concentration of 2.5 ×10⁷, 1.25 ×10⁷, 2.5 ×10⁶, 1.25 ×10⁶ and 2.5 ×10⁵ PIBs/mL were 7.49, 7.94, 9.08, 9.57 and 10.01 days, respectively.A study on larval susceptibility to a concentration of 2.5 ×10⁶ PIBs/mL virus suspension indicated that the LT₅₀ values increased with the increasing of instar(from 5.97 days for early second instar larvae to 12.57 days for early fourth instar larvae).Mortality decreased with the increasing of instar (96.7 % for early second instar larvae, 73.3 %for early third instar larvae and 30.0% for early fourth instar larvae).Mortality of larvae decreased and the LT₅₀ values increased at a low temperature(16 ℃±1 ℃) and at a high temperature(35 ℃±1℃)in contrast to a middle temperature(21 ℃±1 ℃or 26 ℃±1 ℃). The field-release testing were conducted on 8th of May, 1998, when 85% of the larval population consisted of the second and third instar larvae and on 17th of May, 1998, when 85% of the larval population consisted of the third and fourth instar larvae, with virus suspension 2.5 ×10⁶, 5 ×10⁶, 2.5 ×10⁷ and 5 ×10⁷. The adjusted mortility were 90.33%, 90.35 %, 91.80 %, 92.29 % for the first spraying and 66.03 %, 67.31 %, 65.69 %, 69.38 % for the second spraying, respectively, which were analyzed statistically of ANOVA and Duncan' s new multiple-range test.The results from field-release testing indicated that the best time for control Chihuo zao Yang was the first ten-days of May in Taigu, Shanxi and the concentration suitable for control was 2.5 ×10⁷ PIBs/mL, with which the adjusted mortility was higher and the cost was lower.

Key words: Chihuo zao Yang, Nuclear polyhedrosis virus, Bioassay, Field efficacy tests

冀卫荣,刘贤谦,师光禄. 枣尺蠖核多角体病毒活性及林间防治试验[J]. 林业科学, 1999, 35(6): 81-85.

Weirong Ji,Xianqian Liu,Guanglu Shi. THE BIOASSAY AND FIELD EFFICACY TESTS OF THE NUCLEAR POLYHEDROSIS VIRUS OF Chihuo zao Yang[J]. Scientia Silvae Sinicae, 1999, 35(6): 81-85.

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