欢迎访问林业科学,今天是

林业科学 ›› 1999, Vol. 35 ›› Issue (6): 81-85.

•   • 上一篇    下一篇

枣尺蠖核多角体病毒活性及林间防治试验

冀卫荣,刘贤谦,师光禄   

  1. 山西农业大学 太谷 030801
  • 收稿日期:1998-11-09 出版日期:1999-11-25 发布日期:1999-11-25
  • 基金资助:
    山西省科技攻关项目(972086)

THE BIOASSAY AND FIELD EFFICACY TESTS OF THE NUCLEAR POLYHEDROSIS VIRUS OF Chihuo zao Yang

Weirong Ji,Xianqian Liu,Guanglu Shi   

  1. Forestry Department, Shanxi Agricultural University Taigu 030801
  • Received:1998-11-09 Online:1999-11-25 Published:1999-11-25

摘要:

本文报道了枣尺蠖(Chihuo zao Yang)核多角体病毒(CzN PV)活性的生物测定结果及在山西太谷枣树林间防治枣尺蠖的试验效果。以不同浓度该病毒悬液浸渍鲜枣树叶,饲喂感染3龄初枣尺蠖幼虫,病毒浓度对数值与死亡机率的回归直线方程为; LC50及95 %的置信限分别为3.65 ×105及4.04 × 104 ~ 3.13 ×106PI Bs/ mL。以2.5 ×105、1.25×106、2.5 ×106、1.25 ×107和2.5 ×107PIBs/mL 5种浓度病毒感染3龄初幼虫,其LT50值分别为10.01、9.57、9.08、7.94和7.49 d。同一浓度病毒(2.5 ×106 PI Bs/ mL)对不同虫龄感染力不同。幼虫死亡率随虫龄增大而降低,从2龄的96.7%降低到4龄的30.0 %; LT50值随着虫龄增大而增大,从2龄的5.97 d增加到4龄的12.57 d。在山西太谷枣林枣尺蠖幼虫2龄、3龄占85 %时喷洒浓度为2.5 ×107P IBs/ mL的病毒悬液,虫口减退率高,成本较低,可在生产上推广应用。

关键词: 枣尺蠖, 核多角体病毒, 生物活性测定, 林间防治试验

Abstract:

The results of bioassay and field efficacy tests of the nuclear polyhedrosis virus of Chihuo zao Yang were reported in this paper.Early third-instar larvae were fed on jujube tree leaves immersed with polyhedral inclusion bodies(PIB)suspension of virus.Mortality of larvae increased with the increasing of concentration of polyhedral inclusion bodies suspension.The LC50 and 95% confidence limits were 3.65 × 105 and 4.04 ×104 ~ 3.13 ×106PIBs/mL, respectively.The LT50 values for the virus concentration of 2.5 ×107, 1.25 ×107, 2.5 ×106, 1.25 ×106 and 2.5 ×105 PIBs/mL were 7.49, 7.94, 9.08, 9.57 and 10.01 days, respectively.A study on larval susceptibility to a concentration of 2.5 ×106 PIBs/mL virus suspension indicated that the LT50 values increased with the increasing of instar(from 5.97 days for early second instar larvae to 12.57 days for early fourth instar larvae).Mortality decreased with the increasing of instar (96.7 % for early second instar larvae, 73.3 %for early third instar larvae and 30.0% for early fourth instar larvae).Mortality of larvae decreased and the LT50 values increased at a low temperature(16 ℃±1 ℃) and at a high temperature(35 ℃±1℃)in contrast to a middle temperature(21 ℃±1 ℃or 26 ℃±1 ℃). The field-release testing were conducted on 8th of May, 1998, when 85% of the larval population consisted of the second and third instar larvae and on 17th of May, 1998, when 85% of the larval population consisted of the third and fourth instar larvae, with virus suspension 2.5 ×106, 5 ×106, 2.5 ×107 and 5 ×107. The adjusted mortility were 90.33%, 90.35 %, 91.80 %, 92.29 % for the first spraying and 66.03 %, 67.31 %, 65.69 %, 69.38 % for the second spraying, respectively, which were analyzed statistically of ANOVA and Duncan' s new multiple-range test.The results from field-release testing indicated that the best time for control Chihuo zao Yang was the first ten-days of May in Taigu, Shanxi and the concentration suitable for control was 2.5 ×107 PIBs/mL, with which the adjusted mortility was higher and the cost was lower.

Key words: Chihuo zao Yang, Nuclear polyhedrosis virus, Bioassay, Field efficacy tests