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林业科学 ›› 2019, Vol. 55 ›› Issue (4): 122-128.doi: 10.11707/j.1001-7488.20190412

• 论文与研究报告 • 上一篇    下一篇

基于28S rDNA分析板栗和杉木上针叶小爪螨物种分化原因

尹淑艳1,2, 李波3, 周成刚1,2, 张卫光1, 谢丽霞1, 刘永杰1   

  1. 1. 山东农业大学植物保护学院 泰安 271018;
    2. 山东省林业有害生物防控工程技术研究中心 泰安 271018;
    3. 泰安市林业局 泰安 271000
  • 收稿日期:2017-09-04 修回日期:2018-02-08 出版日期:2019-04-25 发布日期:2019-04-30
  • 基金资助:
    山东省高等学校科技计划项目(J14LE14);国家重点研发计划项目(2016YFD0201113);国家自然科学基金项目(31401957)。

Analysis on Species Differentiation of Oligonychus ununguis on Castanea mollissima and Cunninghamia lanceolata based on 28S rDNA Gene Sequences

Yin Shuyan1,2, Li Bo3, Zhou Chenggang1,2, Zhang Weiguang1, Xie Lixia1, Liu Yongjie1   

  1. 1. College of Plant Protection, Shandong Agricultural University Tai'an 271018;
    2. Engineering Research Center of Forest Pest Management of Shandong Province Tai'an 271018;
    3. Forestry Bureau of Taian City Tai'an 271000
  • Received:2017-09-04 Revised:2018-02-08 Online:2019-04-25 Published:2019-04-30

摘要: [目的]我国经济林树种板栗和杉木上的重要害螨一直被认为是同一种螨——针叶小爪螨,但针叶小爪螨的山东板栗种群和浙江杉木种群间存在生殖隔离,且生殖隔离并非由地理隔离和能调控寄主生殖的内共生菌引起,似乎二者已分化为2个独立的种。本研究目的是明确其成种原因,丰富物种形成理论。[方法]从之前做杂交试验时所采集的针叶小爪螨板栗和杉木种群中各随机取3头雌成螨,提取单头雌成螨的总DNA,使用根据其他小爪螨28S rDNA两端保守序列设计的引物扩增28S rDNA,扩增产物纯化后测序。比较2种群的28S rDNA序列,并基于28S rDNA序列构建小爪螨属系统发育树,结合已有研究分析两种群分化原因。[结果]每个种群的3个个体的28S rDNA序列完全一致,无种群内变异。2种群的28S rDNA序列一致性为98.3%,遗传距离为1.7%。在基于28S rDNA序列构建的小爪螨属系统发育树上,浙江杉木种群与采自日本的日本柳杉上的本岛小爪螨亲缘关系最近,山东板栗种群与采自日本的日本栗上的栗小爪螨的亲缘关系最近。[结论]板栗和杉木上的叶螨并非由同一种叶螨因适应不同寄主植物分化而成,很可能是2个独立的物种。

关键词: 针叶小爪螨, 28S rDNA, 板栗种群, 杉木种群, 物种分化

Abstract: [Objective] The important pest mites that infest Castanea mollissima,an economic forest species, and Cunninghamia lanceolata, have been considered to be the same mites, Oligonychus ununguis. However, previous studies have shown that reproductive isolation exists between the C. mollissima population from Shandong Province(TSBL) and the C. lanceolata population from Zhejiang Province(ZJSM) of O. ununguis and the reproductive isolation is not caused by geographic isolation and the endosymbionts that can manipulate the reproduction of their hosts. The two populations have been differentiated into two independent species, but whether the differentiation is related to adaptation to different host plants has not been determined. This study was carried out to clarify the differentiation reasons and enrich the speciation theory.[Method] Three female adult mites were randomly selected from the C. mollissima population and the C. lanceolata population respectively which collected previously to be used to hybridization experiment. The genomic DNA was extracted from a single female adult. The 28S rDNA sequences were amplified used the primers designed according to the conserved sequences of the 28S rDNA of other mites in Oligonychus. The amplified products were purified and sequenced. The 28S rDNA sequences of the two populations were compared and the phylogenetic tree of Oligonychus was constructed based on the 28S rDNA sequences. The differentiation reasons of these two populations were analyzed combined with previous studies.[Result] The 28S rDNA sequences of three individuals in each population were identical, without intraspecific variation. The interpopulation sequence identity and the genetic distance between the C. mollissima population and the C. lanceolata population based on 28S rDNA sequences were 98.3% and 1.7% respectively. The phylogenetic tree of Oligonychus based on 28S rDNA sequences showed that O. hondoensis collected from Cryptomeria japonica in Japan was the closest relative of the C. lanceolata population, while O. castaneae collected from Castanea crenata in Japan was the closest relative of the C. mollissima population.[Conclusion] The spider mites on C. mollissima and C. lanceolata are probably two independent species rather than differentiated from the same kind of spider mite because of adaptation to different host plants.

Key words: Oligonychus ununguis, 28S rDNA, Castanea mollissima population, Cunninghamia lanceolata population, species differentiation

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