纤维素基质固载酶材料制备及固定化漆酶性能

doi:10.11707/j.1001-7488.20131117

林业科学 ›› 2013, Vol. 49 ›› Issue (11): 122-128.doi: 10.11707/j.1001-7488.20131117

纤维素基质固载酶材料制备及固定化漆酶性能

郭明¹, 燕冰宇¹, 王春鹏², 周建钟¹

1. 浙江农林大学化学系临安 311300;
2. 中国林业科学研究院林产化学工业研究所南京 210042

收稿日期:2012-12-31 修回日期:2013-03-12 出版日期:2013-11-25 发布日期:2013-11-26

Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance

Guo Ming¹, Yan Bingyu¹, Wang Chunpeng², Zhou Jianzhong¹

1. Department of Chemistry, Zhejiang Agriculture & Forestry University Lin'an 311300;
2. Institute of Chemical Industry of Forest Products, CAF Nanjing 210042

Received:2012-12-31 Revised:2013-03-12 Online:2013-11-25 Published:2013-11-26

摘要/Abstract

摘要：

以微晶纤维素（MCC）为原料，采用新型水热氧化合成方法，制备纤维素基质固定化漆酶载体材料——双醛纤维素（DAC）。红外光谱（IR）、固体核磁共振（CP/MAS¹³C NMR）、X-射线衍射（XRD）和扫描电镜（SEM）表征产物结构。测试制备的纤维素基质固载漆酶的酶学性能，并与交联壳聚糖、双醛淀粉固载漆酶的酶学性能进行比较分析，构建酶学性能理论方程。结果表明：新型水热氧化反应成功制备了高醛基含量的氧化纤维素，所得氧化纤维素载体材料固定化漆酶与交联壳聚糖和双醛淀粉固定化漆酶相比，稳定性好，重复使用率高，可在较宽温度和pH范围内保持酶活性，建立的理论方程较好表征了固载酶酶学性能。

关键词: 双醛纤维素, 水热合成, 固定化漆酶, 结构表征, 酶学性能

Abstract:

Dialdehyde cellulose(DAC), a carrier material of cellulose-based immobilized laccase using microcrystalline cellulose(MCC)as raw materials was prepared by new hydrothermal oxidation synthesis method. The structure was characterized by IR, CP/MAS¹³C NMR, XRD and SEM. The enzymatic characteristics of cellulose-based immobilized laccase were tested and compared with chitosan-based immobilized laccase and dialdehyde starch-based immobilized laccase. The theoretical equations of enzymatic characteristics were established. The experimental results showed that DAC containing high aldehyde group content was prepared successfully using hydrothermal oxidation synthesis method. The dialdehyde cellulose-based immobilized laccase had better affinity ability, higher reusage and maintained enzyme activity within broader temperature and pH regions compared with chitosan-based immobilized laccase and dialdehyde starch-based immobilized laccase. The theoretical equations were well able to characterize the enzymatic characteristics.

Key words: dialdehyde cellulose (DAC), hydrothermal synthesis, immobilized laccase, structure characterization, enzymatic characteristics

中图分类号:

TQ352.79
Q55

郭明, 燕冰宇, 王春鹏, 周建钟. 纤维素基质固载酶材料制备及固定化漆酶性能[J]. 林业科学, 2013, 49(11): 122-128.

Guo Ming, Yan Bingyu, Wang Chunpeng, Zhou Jianzhong. Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance[J]. Scientia Silvae Sinicae, 2013, 49(11): 122-128.

参考文献

王炎,郑旭翰,赵敏. 2008. 漆酶在介孔分子筛MCM-41上的固定化研究. 高校化学工程学报, 22(1): 83-87.
杨宇翔, 张亚妮, 邵建国, 等. 2011. 介孔SiO₂固定化漆酶降解2,4-DCP. 同济大学学报: 自然科学版, 39(9): 1324-1328.
赵敏,王玮,李昕芯,等.2008. 海藻酸钠磁性微粒的制备及对漆酶的固定化. 中国造纸学报, 23(4): 94-99.
Alemdar A, Sain M. 2008. Isolation and characterization of nanofibers from agricultural residues-wheat straw and soy hulls. Bioresource Technology, 99(6): 1664-1671.
Duron N, Rosa M A, Annibale A. 2002. Applications of laccases and tyrosinases immobilized on different supports: a review. Enzyme and Microbial Technology, 31(7): 907-931.
Giardina P, Faraco V, Pezaella C. 2010. Laccases: a never ending story. Cellular and Molecular Life Science, 67(3): 369-385.
Krajewska B. 2009. Ureases. II. Properties and their customizing by enzyme immobilizations: a review. Journal of Molecular Catalysis B:Enzymatic, 59(1/3): 22-40.
Noriyuki I, Lee D S, Kwon Y J, et al. 2011. Immobilization of protein on cellulose hydrogel. Cellulose, 18(5): 1251-1256.
Oh S Y, Yoo D I, Shin Y, et al. 2005. Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy. Carbohydrate Research, 340(15): 2376-2391.
Princi E, Vicini S, Pedemonte E. 2004. Physical and chemical characterization of cellulose based textiles modified by periodate oxidation. Macromolecular Symposia, 218(6): 343-352.
Qiu L F, Huang Z X. 2010. The treatment of chlorophenols with laccase immobilized on sol-gel-derived silica. World Journal of Microbiology and Biotechnology, 26(5): 775-781.
Sirvio J, Liimatainen H, Niinimaki J, et al. 2011. Dialdehyde cellulose microfibers generated from wood pulp by milling-induced periodate oxidation. Carbohydrate Polymers, 86(1): 260-265.
Shi X Z, Huang M, Zhao Z F. 2011. Nonlinear Fitting Technology of 7-parameter cross-WLF viscosity model. Advanced Materials Research, 189: 2103-2106.
Tavolaro A, Riccio L L, Tavolaro P. 2012. Hydrothermal synthesis of zeolite composite membranes and crystals as potential vectors for drug-delivering biomaterials. Microporous and Mesoporous Materials, 167: 62-70.
Yang Z P, Si S H, Zhang C J. 2008. Study on the activity and stability of urease immobilized onto nanoporous alumina membranes. Microporous and Mesoporous Materials, 111(1/3): 359-366.
Yuan L I, Emiko R, Selvi T. 2012. Detection of clarithromycin resistance in Helicobacter pylori following noncryogenic storage of rapid urease tests for 30 days. Digestive Diseases, 13(1): 54-59.

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纤维素基质固载酶材料制备及固定化漆酶性能

Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance

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