臭椿花叶病病原病毒的研究

›› 1993, Vol. 29 ›› Issue (6): 503-508.

臭椿花叶病病原病毒的研究

姚俊梅刘仪周仲铭

北京林业大学,北京100083；北京农业大学,北京100094；北京林业大学,北京100083

收稿日期:1991-01-01 修回日期:1900-01-01 出版日期:1993-11-25 发布日期:1993-11-25

THE OCCURRENCE OF A POTYVIRUS IN AILANTHUS ALTISSIMA MILL,IN CHINA

Yao Junmei Liu Yi Zhou Zhongming

Beijing Forestry University,Beijing 100083;Beijiag Agricultural University,Beijing 100094;Beijing Forestry University, Beijing 100083

Received:1991-01-01 Revised:1900-01-01 Online:1993-11-25 Published:1993-11-25

摘要/Abstract

摘要： 从出现花叶症状的臭椿上分离到一种线条状病毒,经过生物学特性、病毒粒体形态、血清学及超薄切片等方面的研究,确定为马铃薯Y病毒组病毒。

关键词: 臭椿, 马铃薯Y组病毒, 树木病毒病

Abstract: It a fleuxous filamentous virus has been isolated from Ailanthus altissimaMill. showing mosaic symptoms. It was identified as a menber of potyvirusgroup. The virus could infect twenty-one of the twenty-three plant speciescultivas from six families inoculated. The thermal inactivation point was 70-75℃. The longevity in vitro was longer than 5 weeks. The dilution endpoint was 10^-3-10^-4. It was easily transmitted by Myzus persicae Sulz. Thevirus particles were fleuxous rods, about 725nm long. The purified prepareation had an ultraviolet absorption spectra typical of a nucleoprotein (A=260/280=1.20). The virus capsid contained a single protein species with amolecular weight of 35,000 daltons. Antisera to the virus was obtained fromrabbits immunized with purified virus preparations. The virus could be"described" in ISEM tests using antisera for PVY. In addition, electron microscopyof thin sections of infected leaf tissue demonstrated the presence of severaltypes of inclusion bodies similar in appearance to inclusions prevously descri-bed in association with potyvirus infections in other plants.

Key words: Ailanthus altissima, Potyvirus, Virus disease

姚俊梅刘仪周仲铭. 臭椿花叶病病原病毒的研究[J]. , 1993, 29(6): 503-508.

Yao Junmei Liu Yi Zhou Zhongming. THE OCCURRENCE OF A POTYVIRUS IN AILANTHUS ALTISSIMA MILL,IN CHINA[J]. , 1993, 29(6): 503-508.