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›› 1988, Vol. 24 ›› Issue (3): 268-274.

• 论文及研究报告 • 上一篇    下一篇

杨树柳树叶绿体类囊体膜蛋白的研究

种克雄 徐纬英   

  1. 中国林业科学研究院林业研究所
  • 收稿日期:1986-03-07 修回日期:1900-01-01 出版日期:1988-08-25 发布日期:1988-08-25

A STUDY ON THE CHLORPLAST THYLAKCID MEMBRANE PROTEINS OF POPLAR AND WILLOW

Chong Kexiong,Xu Weiying   

  1. Forest Research Institute,Chinese Academy of Forestry
  • Received:1986-03-07 Revised:1900-01-01 Online:1988-08-25 Published:1988-08-25

摘要: 本实验以杨树几个代表种及杨树杂交组合‘美杨×小叶杨’的正反交杂种和亲本,柳树杂交组合‘垂柳×爆竹柳’杂种及其亲本为材料,利用十二烷基磺酸钠(SDS)-聚丙烯酰胺凝胶电泳(PAGE)方法,比较分析了叶绿体类囊体膜蛋白表征,及其在亲子代之间的遗传表现和雌雄性别间的差异。结果表明,杨树叶绿体类囊体膜蛋白质的SDS-PAGE,可显示出40多条带,不同种间,膜蛋白成分存在差异。根据差异的表现,可以将供试的杨属的几个代表种划分为三组。此外,杨树雌雄性别间膜蛋白组成有差异,但不同代表种表现各不相同,没有得到一定的共同规律。小叶杨雄株比雌株多出一条稳定的分子量为63kd多肽带,可以作为小叶杨的雄性生化标志。在杨树杂交组合中,分子量为44kd的多肽带表现为偏母遗传。柳树杂种形态性状分离与其膜蛋白组成成份分离相吻合,说明细胞质对树木杂种性状的遗传具有一定的相关作用。杂种出现特异多肽带,说明膜蛋白表征可以作为一个标记,应用于杂种分析和鉴定中。

Abstract: In this experiment,chloroplast thylakoid membrane proteins were isolated from poplar reciprocal hybrids (Poplus siminii×P.pyramidalis;P.pyramidalis×P.simonii) and willow hybrids (Salix babylonica×S.fragilis),as well as their parents,and also several poplar representive species including female and male plants.The polypeptides were separated by SDSPAGE.The polypeptide patterns obtained were analysed and compared between hybrids and their parents,female and male plants,and among the representive species.Results obtained are as follows: 1.poplar thylakoid membrane proteins were separated into more than 40 polypeptides varied with different species.According to this difference.these tested species of Poplus can be divided into three groups:Aigeiros (P.deltoides Bartr CL.'Lux' and P.deltoides Bard CL 'Harvard') and Tacamahaca (P.simonii Carr) possessing 50kd,and 27kd polypeptides,are in the same group,Leuce (P.alba L.) which has no these two polypeptides belongs to the second group.The third group is Leucoides (P.lasiocarpa Oliv.) in which,50kd and 27kd bands are very faint. 2.One 44kd polypeptide band was observed in the hybrid poplar when it is possessed in their female parent but not in their male parent.It means that 44kd polypeptide may be maternal inheritance and be used as a genetic marker in poplar breeding. 3.The patterns of thylakoid membrane polypeptides of willow hybrids were coincided with its parents in morphological characters.Therefore,we can deduce that there are some relations between thylakoid membrene polypeptides and morphological characters. 4.There were no common pattern of thylakoid membrane polypeptides between female and male and male plants in all tested species,But the male plant of P.simonii Carr.has one more polypeptide band than female plant.This band is very stable and good repeatability in our experiment and can be used as a marker to distinguish the male plant of P.simonii Carr.in poplar breeding.