欢迎访问林业科学,今天是

›› 1988, Vol. 24 ›› Issue (2): 156-161.

• 论文及研究报告 • 上一篇    下一篇

杉木硝酸还原酶的初步研究

周国章 苏梦云   

  1. 中国林业科学研究院亚热带林业研究所
  • 收稿日期:1986-09-24 修回日期:1900-01-01 出版日期:1988-05-25 发布日期:1988-05-25

A PRELIMINARY STUDY ON NITRATE REDUCTASE ACTIVITY IN THE LEAVES OF CUNNINGHAMIA LANCEOLATA

Zhou Guozhang,Su Mengyun   

  1. Subtropical Forest Research Institute,Chinese Academy of Forestry
  • Received:1986-09-24 Revised:1900-01-01 Online:1988-05-25 Published:1988-05-25

摘要: 本项研究表明,在两种生长速率不同的杉木无性系间,硝酸还原酶活力存在显差异。一般硝酸还原酶活力高的无性系生长快,新梢平均生长为59cm和76cm。两类无性系植株的硝酸还原酶活力分别为0.08和0.236μmolNO2-·h(-1)·g鲜重(-1)。硝酸还原酶活力随生长时期变化,生长旺盛时期(8月)硝酸还原酶活力最高。一年生枝条上叶片的硝酸还原酶活力要比二年生枝条上叶片的硝酸还原酶高。当年生主干的新生枝上的成龄叶片,其硝酸还原酶活力高于去年和前年生主干的新生枝上的成龄叶片。当年生枝条中部的成龄叶硝酸还原酶活力明显高于枝顶的未成龄叶片和枝条基部角质化老叶的硝酸还原酶活力。试验结果,认为硝酸还原酶活力作为生长速率的生化指标是可能的。

Abstract: There is significant difference in nitrate reductase (NR) activity between clones of Chinese fir with different growth rate.Generally,high NR activity was found in the fast growing clone.Trees with new shoot mean growth of 59 and 76 cm had NR activity values of 0.08 and 0.236 μ mol NO2- per g fresh weight per hour respectively.The level of NR activity varied with the growing period.The maximum activity was observed when the tree is quick growing (Aug.).The NR activity in the leaves on 1 year old branch was higher than that in the leaves on two years old branch.The NR activity in mature leaves on current lateral branches from stem of current year growth higher than that from stem of last year growth and the year before last,which in mature leaves from the middle of the branch of current growth significantly higher than that in young leaves from the top and that in cutinized leaves from the base of the same branch.If the branch with the leaves on were induced with potassium nitrate solution and light,NR activity in leaves increased apparently.Higher NR activity was obtained when treated with 50 m mol/L KNO3 and illumination of 2000 lux for 12 hours.According to the results obtained in these exprements,it is suggested that NR activity could be used as a biochemical indicator of growth rate of Chinese fir.