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›› 1987, Vol. 23 ›› Issue (4): 443-447.

• 论文及研究报告 • 上一篇    下一篇

波纹杂毛虫核多角体病毒的研究

黄健屏   

  1. 中南林学院
  • 收稿日期:1985-12-15 修回日期:1900-01-01 出版日期:1987-11-25 发布日期:1987-11-25

STUDIES ON A NUCLEAR POLYHEDROSIS VIRUS OF OAK CATERPILLAR AND ITS NUCLEAR ACID-DNA

Huang Jianping   

  1. Central-South Forestry College
  • Received:1985-12-15 Revised:1900-01-01 Online:1987-11-25 Published:1987-11-25

摘要: 波纹杂毛虫(Cyclophragma undans Walker)幼虫感染波纹杂毛虫核多角体病毒(简称CuNPV)初期,行动呆滞,食欲不振,体色变为深褐色。死后内脏液化,体壁易破,流出茶色脓液,不臭。室内毒效测定,CuNPV0.4×108PIB/ml能使6—8龄波纹杂毛虫幼虫死亡率高达94.7%。CuNPV的多角体呈三角形、四边形、五边形和不规则的多边形。大小为1.8—2.9μm,少数可达4.0μm。多粒包埋,多数为2—4个。病毒粒子杆状,大小为250—300×30—40nm。多角体蛋白质晶格为点线型,两点的中心距离约为4.4nm。晶格点的直径为2.5nm。病毒束外面有一层共同的疏松蛋白质膜包围,膜厚为15—20μm。病毒粒子经直接释放的DNA,在电镜下呈松弛的开环状分子和闭环状分子,平均长度为36.1μm。分子量为71.12×106道尔顿。经试验鉴定本病毒为CuNPV。CuNPV的安全试验结果表明,对脊椎动物未发现不安全之处。

Abstract: A series of experiments with a newly finding nuclear polyhedrosis virus of the oak caterpillar (Cyclophragma undans Walker) have been carried out. This virus, causes the larvae of the oak caterpillar lose their appetite and become slow in movement and yellowish brown in color after infection. The inne tissues of the dead larvae were fluidized and flowed out from the damaged skins which were fragile. The laboratory virulence tests showed that the mortality of the 6—8th instar larvae come up to 94.7% trated with 0.4×108 PIB/ml CuNPV.Under electronic microscope the polyhedral bodies of CuNPV are tetrangonal, pentagonal or irregularly polygonal, and are measured to be 1.8—2.9μm, sometimes up to 4.0μm in size. Their protein lattice is of a pattern of regular dots and rows. The distance of two neighouring grides of the lattice is 4.40nm. The dots of the protein lattice are about 2.5nm in diameter. The virus bundle is enveloped by lax protein capsule with a thickness of 15—20nm. The polyhedroses are MEV, most of them have 2—4 virions which are rod-shaped and are measured 250—300×30—40nm.The viral DNA released from virions of CuNPV are found to be the circular or open circular molecules with countour length of 36.1 μm on the average. The molecular weight of the viral DNA is 71.12×106 daltons.This virus as an insecticide is safe to vertebrates by experiments.