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›› 1986, Vol. 22 ›› Issue (3): 291-294.

• 研究简报 • 上一篇    下一篇

泡桐杂种叶片培养中愈伤组织的诱导和不定芽的形成

黄钦才 林静芳 董茂山 桂耀林 顾淑荣 徐廷玉   

  1. 中国林业科学研究院林业科学研究所;中国科学院植物研究所
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-08-25 发布日期:1986-08-25

CALLUS INDUCTION AND FORMATION OF ADVENTITIOUS BUDS OF PAULOWNIA ELONGALA×P. TOMENTOSA IN VITRO

Huang Qincai,Lin Jingfang,Dong Maoshan,Gui Yaolin,Gu Shurong,Xu Tingyu   

  1. Forest Research Institute, Chinese Academy of Forestry;Institute of Botany, Academia Sinica
  • Received:1900-01-01 Revised:1900-01-01 Online:1986-08-25 Published:1986-08-25

Abstract: Leaves of Paulownia elongala×P. tomentosa were used as experimental material. Plantlets were obtained from MS medium supplemented with 6-BA 4.5ppm+NAA0.2ppm+CH 500ppm.The stem of plantlet was cut off when it reached 2cm in height and was transferred on MS medium with NAA 0.03ppm for rooting. The frequency of rooting is higher on liquid medium with filter paper bridge than solid agar medium. It reached 90% and survived easily when the plantlet with roots was transferred to soil.Histocytological observations on the adventitions buds for mation were also discussed in this paper.