油橄榄炭疽病菌C. gloeosporiodes毒素对油橄榄原生质体的毒害作用

›› 1986, Vol. 22 ›› Issue (1): 30-37.

油橄榄炭疽病菌C. gloeosporiodes毒素对油橄榄原生质体的毒害作用

王敬文

中国林业科学研究院亚热带林业科学研究所

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-01-25 发布日期:1986-01-25

STUDIES ON THE TOXIN FROM COLLETOTRICHUM GLOEOSPORIODES INJURING OLIVE PROTOPLAST

Wang Jingwen

Subtropic Forest Research Institute, Chinese Academy of Forestry

Received:1900-01-01 Revised:1900-01-01 Online:1986-01-25 Published:1986-01-25

摘要/Abstract

摘要： 在改良的Czapek-Dox培养液中,用振荡法培养Colletotrichum gloeosporiodes菌8天,从培养滤液中分离制备C. gloeosporiodes毒素。毒素制备物使蕃茄切条发生萎蔫,使油橄榄愈伤组织的原生质体遭受毒害,停止生长和坏死。抗病品种和感病品种的愈伤组织的原生质体对C. gloeosporiodes毒素的敏感性不同,抗病品种“佛奥”的原生质体对毒素100mg/ml浓度的抵抗力约为感病品种“钟山24”原生质体的3倍。Fe⁽⁺⁺⁺⁾能够强化毒素的毒害作用,Cu⁽⁺⁺⁾则弱化毒素的毒害作用。

Abstract: The toxic substances are isolated and partially purified from culture filtrate of Colletotrichum gloeosporioides. The toxic preparation induces tomato seedling to wilt and stoped growth of Olive Callus following necrosis. Olive protoplasts are obtained from callus and exposured in toxin and subsequently examined survival of protoplast in optical microscope. The sensitivity of callus and protoplast to toxin is different in resistant and susceptible var. and the survival of protoplast from resistant var. "Frandoio" is more two-fold than the protoplast from susceptible var. "Zhongsan 24" during 48hr. At 100mg/ml toxin concentration.

王敬文. 油橄榄炭疽病菌C. gloeosporiodes毒素对油橄榄原生质体的毒害作用[J]. , 1986, 22(1): 30-37.

Wang Jingwen. STUDIES ON THE TOXIN FROM COLLETOTRICHUM GLOEOSPORIODES INJURING OLIVE PROTOPLAST[J]. , 1986, 22(1): 30-37.