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›› 1983, Vol. 19 ›› Issue (3): 292-301.

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植树造林与组织培养——通过组织培养繁殖木本植物

王怀智   

  1. 中国科学院上海植物生理研究所
  • 出版日期:1983-08-25 发布日期:1983-08-25

FORESTATION AND PLANT TISSUE CULTURE

Wang Huai-zhi   

  1. Shanghai Institute of Plant Physiology,Academia Sinica
  • Online:1983-08-25 Published:1983-08-25

摘要: 迄今,已有124种木本植物采用离体培养方法培养成再生植株,有些树种的优树繁殖的试管植物已开始用于造林。本文对快速无性繁殖木本植物的方法与概况作了简单的介绍。组织培养方法繁殖木本植物的类型有无菌短枝扦插,器官发生和胚状体发生3种。其特点是繁殖速度较常规无性繁殖快,并可用于繁殖或保存难以繁殖的优良珍贵树种;茎尖培养结合高温处理等措施可以得到去除危害病毒的检定苗,这在果树、林木生产上很有应用价值。目前,在木本植物的组织培养方面除了研究有关细胞、组织和器官的培养条件外,对经济树种,特别是果树与木本油料植物以及与能源有关的树种的快速无性繁殖研究颇受重视。

Abstract: This reivew is a survey in tissue culture of forest trees and the methods of clonal propagation of woody plants.Tissue culture offers an important technique for the rapid clonal propagation of forest trees.So far about 124 species belong to 30 families of woody plants have been regenereted as plantlets.Significant progress has been made in the past six years in vitro propagated conifers.In addition,a list over 70 species of woody plants may be cloned by somatic embryogenesis. The three principal types of clonal propagation of woody plants were discussed:aseptic shoot cuttage,organogenesis and embryogenesis.Eucalyptus (Eucalyptus leickow №1) regenerated in vitro via embrogenesis has been applied to forestation in Guangxi Region of our country. Finally,materials of the woody plants propagation were briefly reviewed in the 5th IAPTC.