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›› 1983, Vol. 19 ›› Issue (2): 121-125.

• 论文及研究报告 • 上一篇    下一篇

坡垒、青皮种子失水过程中活力与根尖细胞亚显微结构变化研究

宋学之 陈青度 王东馥 杨军   

  1. 中国林业科学研究院热带林业研究所;中国林业科学研究院林业科学研究所
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1983-05-25 发布日期:1983-05-25

A STUDY OF ULTRASTRUCTURAL CHANGES IN RADICLE-TIP CELLS AND SEED VIGOR OF HOPEA AND VATICA IN LOSING WATER PROCESS

Song Xue-zhi,Chen Qing-du,Wang Dong-fu,Yang Jun   

  1. Institute of Tropical Forestry,Chinese Academy of Forest Science;Institute of Forestry,Chinese Academy of Forest Science
  • Received:1900-01-01 Revised:1900-01-01 Online:1983-05-25 Published:1983-05-25

摘要: 坡垒(Hopea hainanensis Merr.et Chun.)、青皮(Valica astrotricha Han-ce)种子不能长期干藏,经作者试验研究,原因在于经过干藏种子含水率迅速下降,坡垒种胚及子叶颜色变暗,韧性降低变脆,无光泽。根尖细胞亚显微结构明显变化,当含水量从34.0%降至20.3%以下时细胞质空泡化,细胞核的结构正常,然后液泡膜破裂,核内结构变得模糊,直至质壁分离,核膜和核仁的界限不分明,染色体均质化,呈黑色一团,各种细胞内的物质已大量消失,剩下胞壁或仅含某些分解残物。青皮种子根尖细胞当水量下降至23.1%时许多细胞处于解体。作者认为:坡垒、青皮种子之所以因干燥而降低活力,除有人解释为蛋白质变性成为不可逆转的凝胶以外,而且在失水过程中,其细胞的亚显微结构受到不同程度的破坏。坡垒、青皮植物的种实在成熟后无休眠现象,仍需保持很高的含水量(40—50%),只能湿藏。

Abstract: After a series of seeds of Hopea hainanensis or Vatica astrotricha were treatedunder artificial drying for different days at 25℃ or 20℃,to reduce theirwater content to what this experiment was designed,two groups of thesetreated seeds were used for comparing their vigor and ultrastructural changes of their radicle-tip cells,respectively.The results obtained are summarized as follows: Following that the water content was reduced continuously,the vigorexpressed as GP(germination percent),GI(germination index)and Ⅵ(vigor index)also decreased obivously for these treated seeds in the germination test.And the morpholgy of embryos and their cotylendons changed todarkening and wilting in different extents. For Hopea hainanensis,when its water content was reduced from 34.9%to 25.6% and GP decreased from 65% to 20%,the ultrastructures ofradicle-tip cells showed significant changes: (1) The cytoplasm formed a lot of vesicles,but the fine structure ofnucleus seemed to be normal. (2) The tonoplast ruptured,the structures in the cell became indistinct. (3) The cytoplasm severely segregated with cell wall,nuclear membrane and nucleolus could not be distinguished. When the seed water content was continuously reduced to 20.3% and they had all died,all the structures in the cell were completely destroyed,cytoplasm was disintegrated.Most of substances in some cells had disappeared;and for a great number of cells,there only the cell wall remained. For Vatica astrotricha,its vigor and ultrastructures in radicle-tip cellschanged similarly in the main as Hopea hainanensis.