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›› 1983, Vol. 19 ›› Issue (1): 25-29.

• 论文及研究报告 • 上一篇    下一篇

茶树子叶离体培养形成胚状体的研究

颜慕勤 陈平   

  1. 广西僮族自治区林业科学研究所生理研究室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1983-02-25 发布日期:1983-02-25

STUDIES ON DEVELOPMENT OF EMBRYOID FROM THE CULTURE COTYLEDONS OF TUBA SINENSIS L.

Yan Mu-qin,Chen Ping   

  1. Forest Research rnstitute of the Guangxi Zhuang Nationality Autonomeus Regiou
  • Received:1900-01-01 Revised:1900-01-01 Online:1983-02-25 Published:1983-02-25

摘要: 采用改良的ER培养基,附加不同种类的植物生长调节物质,使茶树接近成熟的种子的子叶基部及子叶柄形成胚状体。详细观察了胚状体形成过程中形态学上和细胞组织学上的变化。初步肯定了胚状体起源于子叶表皮细胞;胚状体在胚胎发生的最初分隔方式上与合子胚不同,发育过程与合子胚较一致。进一步观察到不同种类的植物生长调节物质的用量及配合用量影响胚状体的分化及小植株的形成。并详细介绍了茶树组培苗移栽成活的方法。

Abstract: The use of improved ER medium will enable cotyledons basis and cotyledon petiole of the Thea sinensis L. to develop embryoid. The cytohistological and morphological investigations on the changes in developing process of embryoid have been made in detail. Our works reveal that the embryoid of Thea sinensis L. are differentiated from the epidermal cell of cotyledons. The initial separating patterns of the embryogensis in embryo and embryoid are different. But it js more similar in their form-formative processes. We also found that the different kinds exogenous growth regulators and their quantity used for the cultural medium will affect the differentiations of embryoid and the formation of young plants.