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›› 1982, Vol. 18 ›› Issue (4): 405-408.

• 研究简报 • 上一篇    下一篇

由欧洲赤松(Pinus sylvestris L.)胚离体培养出完整小植株的初步研究

沈熙环 斯·伏·阿诺德   

  1. 北京林学院;瑞典乌布萨拉大学植物生理所
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1982-11-25 发布日期:1982-11-25

IN VITRO FORMATION OF ADVENTITIOUS PLANTLETS FROM EMBRYOS OF Pinus sylvestris L.

Shen Xi-huan,Arnold Sara Von   

  1. Department of Forest Genetics,Beijing Forestry college;Institute of Physiological Botany,University of Uppsala,Swaden
  • Received:1900-01-01 Revised:1900-01-01 Online:1982-11-25 Published:1982-11-25

Abstract: Adventitious bud primordia were formed by culturing isolated embryos of Pinus sylvestris on media containing a relatively high cytokinin to auxin ratio. Further development of the bud Primordia required transfer to media lacking growth regulators.Adventitious buds with a visible stem were isolated from the initial tissue and cultured individually.On the average 10% of the vigorous adventitious shoots formed roots spontaneously after about 10 months in culture.These rooted shoots were transferred to unsterile conditions in the greenhouse.The capacity of forming adventitious buds varied among different seed lots.