欢迎访问林业科学,今天是

林业科学 ›› 2011, Vol. 47 ›› Issue (3): 175-177.doi: 10.11707/j.1001-7488.20110326

• 研究简报 • 上一篇    下一篇

杉木花粉和花粉管中微丝骨架

甘国峰, 徐进, 陆叶, 施季森   

  1. 南京林业大学林木遗传与生物技术省部共建教育部重点实验室 南京 210037
  • 收稿日期:2009-11-06 修回日期:2010-01-18 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 徐进

Microfilament Skeleton in the Pollen and Pollen Tube of Cunninghamia lanceolata

Gan Guofeng, Xu Jin, Lu Ye, Shi Jisen   

  1. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037
  • Received:2009-11-06 Revised:2010-01-18 Online:2011-03-25 Published:2011-03-25

关键词: 杉木, 花粉, 花粉管, 微丝

Abstract:

Microfilament skeleton plays important role during the process of pollen development and pollen tube growth. We observed the distribution of microfilaments in the pollen and pollen tube of Cunninghamia lanceolata by phalloidin labeling fluorescence and confocal laser scanning microscope. The images showed different distribution of microfilaments during pollen germination process. At the early stage of pollen germination, filiform microfilaments occurred in the pollen cytoplasm, and formed thick F-actin bundles around the nuclear surface; With pollen tube growth, microfilament bundles extended and distributed axially in the pollen tube; Thick F-actin bundles were absent near the pollen tube tip and a dense actin cap was formed with thin microfilament fragments in the tip of pollen tube. The microfilament density in the pollen and pollen tube was different from other angiosperms. The results sugguested that the difference in the microfilament density might be related to the slow growth of a pollen tube in C. lanceolata .

Key words: Cunninghamia lanceolata, pollen, pollen tube, microfilaments

中图分类号: