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林业科学 ›› 2010, Vol. 46 ›› Issue (11): 162-167.doi: 10.11707/j.1001-7488.20101125

• 论文 • 上一篇    下一篇

菊花花器性状在F1代变异及相关联的SRAP分子标记

张飞1,2陈发棣1房伟民1陈素梅1   

  1. 1.南京农业大学园艺学院南京210095; 2.浙江省农业科学院花卉研究开发中心杭州311202
  • 收稿日期:2009-09-21 修回日期:2009-12-03 出版日期:2010-11-25 发布日期:2010-11-25
  • 通讯作者: 陈发棣

Variation of Inflorescence Traits in F1 Progeny of Chrysanthemum and the Association with SRAP Markers

Zhang Fei1,2Chen Fadi1Fang Weimin1Chen Sumei1   

  1. 1.College of Horticulture, Nanjing Agricultural UniversityNanjing 210095;2.Flower Research and Development Centre, Zhejiang Academy of Agricultural SciencesHangzhou 311202
  • Received:2009-09-21 Revised:2009-12-03 Online:2010-11-25 Published:2010-11-25

关键词: 菊花, 花器性状, SRAP, 关联分析, 单因素方差分析

Abstract:

Variation of inflorescence traits in F1 progeny populations derived from chrysanthemum (Dendranthema morifolium) cultivars ‘Yuhualuoying’ and ‘Aoyunhanxiao’ were investigated and the association between polymorphic SRAP markers and inflorescence traits was analyzed with One-Way ANOVA. The result showed that the 5 inflorescence traits segregated significantly in the F1 population with coefficient of variation ranging from 15.32% to 49.70%, all fitting into a normal distribution. The association analysis between SRAP markers and inflorescence traits suggested that there were 10, 8, 4, 4 and 5 SRAP markers identified to be significantly related with flower diameter, number of ray florets, number of tubular florets, ray floret length and ray floret width, with the cumulative contribution ratio of 35.781%, 33.702%, 16.175%, 15.018% and 20%, respectively. However, the contribution ratio of each single genetic marker was relatively low, varying from 2.815% to 5.882%, which revealed that these markers were polygene with small effect. We anticipate that the proper utilization of these SRAP markers with relatively large contribution ratio, such as Me4Em1-3, Me4Em9-4, Me12Em3-1 and Me12Em16-5, in future gene-cloning studies would effectively improve molecular breeding program for inflorescence traits in chrysanthemum.

Key words: chrysanthemum(Dendranthema morifolium), inflorescence traits, SRAP, association analysis, one-way ANOVA analysis