不同挥发物诱导的合作杨叶片中POD，PPO及PAL活性变化*

doi:10.11707/j.1001-7488.20091008

林业科学 ›› 2009, Vol. 12 ›› Issue (10): 44-48.doi: 10.11707/j.1001-7488.20091008

不同挥发物诱导的合作杨叶片中POD，PPO及PAL活性变化*

胡增辉^1，2 沈应柏¹ 王宁宁¹ 王金凤¹ 周艳超¹ 张志毅¹

1.北京林业大学生物科学与技术学院北京 100083； 2.北京农学院园林系北京 102206

收稿日期:2008-06-23 修回日期:1900-01-01 出版日期:2009-10-25 发布日期:2009-10-25
通讯作者: 沈应柏
基金资助:

Activities of POD, PPO and PAL in Poplar(Populus simonii×P. pyramidalis ‘Opera 8277’)Leaves Exposed to Different Volatiles

Hu Zenghui^1,2,Shen Yingbai¹,Wang Ningning¹,Wang Jinfeng¹,Zhou Yanchao¹,Zhang Zhiyi¹

1.College of Biological Sciences and Technology, Beijing Forestry University Beijing 100083；2.Garden Department, Beijing University of Agriculture Beijing 102206

Received:2008-06-23 Revised:1900-01-01 Online:2009-10-25 Published:2009-10-25
Supported by:

摘要/Abstract

摘要：

以1年生合作杨扦插苗为试验材料，利用茉莉酸甲酯(MeJA)分别与水杨酸甲酯(MeSA)、顺式己烯醛(cis-hexenal)、反式己烯醛(trans-hexenal)和苯骈噻唑(benzothiazole)混合的4种挥发物熏蒸合作杨叶片，检测过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL) 3种防御酶的活性变化。结果表明：经过4种混合挥发物熏蒸后，合作杨叶片中POD，PPO和PAL活性都有明显的升高，并且2种挥发物混合的诱导效果要高于单独一种挥发物，但不仅仅是每一种挥发物诱导效果的相加之和。从诱导酶活增加的效果来看，在这4种混合挥发物中，MeJA+cis-hexenal的诱导能力最高。

关键词: 合作杨, 混合挥发物, 复合信号, 防御反应, 防御酶活性

Abstract:

The signal transfer between plants was mediated by compound signals composed of different volatiles. In order to explore the action mode of compound signals, one-year-old poplar(Populus simonii×P. pyramidalis ‘Opera 8277’) cuttings were exposed to four mixed volatiles, I.e. methyl jasmonate(MeJA) + methyl salicylate(MeSA), MeJA + cis-hexenal, MeJA + trans-hexenal, MeJA + benzothiazole, and then the induced activities of three defensive enzymes including peroxidase(POD), polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL) were measured. The results showed that all of four mixed volatiles exposure resulted in higher activities of POD, PPO and PAL than only single volatile treatment in intact poplar leaves, indicating that mixed volatiles possessed stronger inducing capability. Moreover the synthetic inducing effect of mixed volatiles was more than the sum of individual volatiles. Among these four mixed volatiles, MeJA + cis-hexenal exhibited the highest inducing capability.

Key words: Populus simonii×, P. pyramidalis &lsquo, Opera 8277&rsquo, mixed volatiles, compound signal, defense response, activities of defensive enzymes

中图分类号:

胡增辉;沈应柏王宁宁王金凤周艳超张志毅. 不同挥发物诱导的合作杨叶片中POD，PPO及PAL活性变化*[J]. 林业科学, 2009, 12(10): 44-48.

Hu Zenghui;Shen Yingbai;Wang Ningning;Wang Jinfeng;Zhou Yanchao;Zhang Zhiyi. Activities of POD, PPO and PAL in Poplar(Populus simonii×P. pyramidalis ‘Opera 8277’)Leaves Exposed to Different Volatiles[J]. Scientia Silvae Sinicae, 2009, 12(10): 44-48.

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不同挥发物诱导的合作杨叶片中POD，PPO及PAL活性变化*

Activities of POD, PPO and PAL in Poplar(Populus simonii×P. pyramidalis ‘Opera 8277’)Leaves Exposed to Different Volatiles

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