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林业科学 ›› 2007, Vol. 43 ›› Issue (03): 34-38.doi: 10.11707/j.1001-7488.20070306

• 论文及研究报告 • 上一篇    下一篇

竹子捕光叶绿素a/b结合蛋白基因全长的克隆和序列分析

高志民1 李雪平1 彭镇华2 岳永德1   

  1. 1.国际竹藤网络中心国家林业局竹藤科学与技术重点开放实验室,北京100102;2.中国林业科学研究院林业研究所,北京100091
  • 收稿日期:2006-11-10 修回日期:1900-01-01 出版日期:2007-03-25 发布日期:2007-03-25

Cloning and Characterization of a Full-Length Gene Encoding the Light Harvesting Chlorophyll a/b-Binding Protein in Bamboo

Gao Zhimin1,Li Xueping1,Peng Zhenhua2,Yue Yongde1   

  1. 1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102; 2. Research Institute of Forestry, CAF Beijing 100091
  • Received:2006-11-10 Revised:1900-01-01 Online:2007-03-25 Published:2007-03-25

摘要:

采用RT-PCR技术与RACE技术,从绿竹中克隆到捕光叶绿素a/b结合蛋白基因全长cDNA,命名为cab-BO1(GenBank登记号EF061137)。该基因长1102bp,从第64~861bp含有1个开放阅读框,编码265个氨基酸。Cab-BO1编码的氨基酸中第64~232位包括典型的捕光叶绿素a/b结合蛋白功能域,第9~11位为蛋白激酶C的磷酸化位点,第27~52位为泛素结构功能域信号,第55~58位为酪氨酸激酶(CK2)磷酸化位点。序列相似性分析结果表明cab-BO1DNA序列和编码的氨基酸序列与玉米、小果野蕉、小麦、水稻等的cab基因及其氨基酸序列的相似性分别在85%和89%以上,显示了cab家族基因在进化过程中的保守性。

关键词: 绿竹, 叶绿素a/b结合蛋白基因(cab), 基因克隆, 序列分析

Abstract:

A full-length Cdna of cab gene was cloned from the first strand of bamboo(Bambusa oldhamii) cDNA through RT-PCR and RACE methods, named as cab-BO1 (cab gene from B. oldhamii). The length of cab-BO1(GenBank accession number:EF061137) is 1 102 bp,which contains an open reading frame encoding 265 amino acids from 64 th to 861 th position. The bioinformatics analysis indicated that the protein encoded by cab-BO1 has a chlorophyll a/b binding domain(64th232th position),a protein kinase C phosphorylation site (9th~11th position), a ubiquitin domain signature (27th~52th position),and a casein kinase II phosphorylation site(55th~58 th position). The DNA sequence and encoding amino acid sequence of cab-BO1 showed high similarity with the cab genes of Triticum aestivum,Musa acuminata,Zea mays and Oryza sativa, more than 85% and 89% repectively. The result indicates a clue that cDNA of cab family genes are conserved.

Key words: bamoo(Bambusa oldhamii), light harvesting chlorophyll a/b-binding protein(cab), gene cloning, characterization