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林业科学 ›› 2003, Vol. 39 ›› Issue (3): 129-135.doi: 10.11707/j.1001-7488.20030321

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利用分子标记技术对林木近缘种进行遗传鉴别的研究

李淑娴 尹佟明 邹惠渝 黄敏仁   

  1. 南京林业大学,南京210037
  • 收稿日期:2001-01-02 修回日期:1900-01-01 出版日期:2003-05-25 发布日期:2003-05-25

DEVELOPING SPECIES-SPECIFIC MOLECULAR MARKERS TO DISTINGUISH CLOSELY RELATED TREE SPECIES

Li Shuxian,Yin Tongming,Zou Huiyu,Huang Minren   

  1. Nanjing Forestry University Nanjing 210037
  • Received:2001-01-02 Revised:1900-01-01 Online:2003-05-25 Published:2003-05-25

摘要:

不同树种近缘种的种子和苗木的真实性鉴别是林木种苗检验过程中常常遇到的难题。本文对国内外林木种苗的真实性鉴别研究及相关信息进行了综述,并在此基础上,对利用种特异分子标记鉴别林木种苗真实性的技术和方法进行了探讨:根据在进化上有同源性的突变基因位点或DNA区段可发展种特异的SNP标记;而利用物种在进化过程中固定的特异基因位点或DNA区段可发展种特异的STS或SCAR标记。这2种类型的种特异分子标记均可直接应用到实际的检验过程中。

关键词: 林木种子检验, 种特异分子标记

Abstract:

The authentic identification of seed and seedling in the closely related tree species is a very difficult problem in the forest tree seed and seedling of inspection. The research proceeding and the related information in this area are reviewed in this paper.On these bases,the authores discuss how to develop species-specific molecular markers to distinguish closely related tree species:one method is to develop species-specific SNPs markers according to the difference of the homogenous genes or loci in different tree species,the other is to develop species-specific STS or SCAR markers due to the specific genes or loci fixed in different tree species in the evolution progress.The species-specific molecular markers developed by these two methods can be used directly in forest tree seed inspection.

Key words: Forest tree seed inspection, Species-specific molecular markers