土壤微生物和氮添加对柠条根际微生境及根系形态的调控作用

doi:10.11707/j.1001-7488.LYKX20230311

Abstract

Abstract:

Objective: This study aims to explore the influence of soil microorganisms on the rhizosphere microbiota characteristics of Caragana korshinskii under nitrogen deposition background, and explore the regulatory rules of microbiota changes on the morphological construction of C. korshinskii root system, so as to provide theoretical basis for ecological restoration of fragile ecosystems. Method: On the basis of soil sterilization treatment (sterilized soil (+S) and non-sterilized soil (-S)), 1-year-old potted C. korshinskii seedlings were subjected to two kinds of inoculation experiments (mixed inoculation with Rhizophagus intraradices and Funneliformis mosseae (+M), and non-inoculation treatment (?M)), and three nitrogen addition treatments (no nitrogen application (0N, 0 g·m^?2a^?1), low nitrogen (LN, 3 g·m^?2a^?1), high nitrogen (HN, 6 g·m^?2a^?1)). After the end of the growing season, the soil microbial biomass, soil enzymes activity related to nutrient conversion, soil nutrient content, and seedling fine root morphology with diameter ≤ 0.5 mm (total root length, total surface area, total volume and number of root tips) of each treatment were analyzed, the correlation between micro ecological environment changes and root morphology construction was explored. Result: 1) In both 0N and LN treatments, the microbial biomass carbon content in rhizosphere soil of C. korshinskii in non-sterilized soil was higher than that in sterilized soil. In HN treatment, the soil microbial biomass carbon content of non-mycorrhizal seedlings was the lowest in sterilized soil. In sterilized soil with different nitrogen addition treatments, the activity of carbon conversion related enzymes in rhizosphere soil of mycorrhizal seedlings was higher than that of non-mycorrhizal seedlings (P<0.05), while the soluble carbon content in rhizosphere soil of C. korshinskii in non-sterilized soil and inoculation treatment was higher than that in the single treatment (P<0.05). 2) Under different nitrogen addition treatments, the nitrogen content of rhizosphere soil microbial biomass of non-mycorrhizal seedlings of C. korshinskii in non-sterilized soil treatment was significantly higher than that in sterilized soil treatment (P<0.05), and the activity of nitrogen conversion related enzymes in rhizosphere soil of C. korshinskii in the interaction of non-sterilized soil treatment and inoculation treatment was significantly higher than that in the single treatment. Under LN and HN treatments, the rhizosphere available nitrogen content of non-mycorrhizal seedlings in soil sterilization was significantly higher than that in other treatments (P<0.05). 3) Under HN treatment, the phosphorus content and alkaline phosphatase activity of microbial biomass in rhizosphere soil of C. korshinskii in the interaction of non-sterilized soil and inoculation treatment were significantly higher than those in other treatments (P<0.05), and the available phosphorus content in rhizosphere soil in the interaction of non-sterilized soil and inoculation treatment was between the single effects of non-sterilized soil and inoculation treatment. 4) Under HN treatment, the root morphological indexes of C. korshinskii mycorrhizal seedlings in sterilized soil were the highest. Under 0N treatment, the root morphological indexes of non-mycorrhizal seedlings in non-sterilized soil were the smallest. 5) The redundancy analysis results showed that microbial biomass carbon content had the highest explanatory degree in the key regulatory factors affecting the root morphology of seedlings, and it had a positive regulatory effect. The results of multiple linear regression analysis showed that alkaline phosphatase activity had the greatest influence on seedling root morphology, and it showed a negative regulatory effect. Conclusion: Under the background of exogenous nitrogen input, the synergistic effect of non-sterilized soil treatment and seedling inoculation treatment is conducive for maintaining the balance of plant rhizosphere microhabitat, and the limiting factor of rhizosphere microhabitat in high nitrogen treatment is transformed into phosphorus element. The root growth of C. korshinskii seedling is promoted by inoculation treatment, but is restricted by soil sterilization treatment. The root growth is closely related to soil carbon and phosphorus cycles process. The root morphological construction is positively regulated by boil microbial biomass, but it is negatively regulated by phosphorus transformation related enzymes.

Key words: Caragana korshinskii, soil sterilization, seedling inoculation, nitrogen addition, rhizosphere microeco-logical environment, root morphology

CLC Number:

S714.3

Shengxi Zhang,Yanhong He,Longfei Hao,Zhengying Nie,Tingyan Liu,Yunpeng Wang,Yongchun Hua. Regulating Effects of Soil Microorganisms and Nitrogen Addition on Rhizosphere Microhabitat and Root Morphology of Caragana korshinskii[J]. Scientia Silvae Sinicae, 2024, 60(9): 80-89.

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References 0

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施入时间 Application time	施入次数 Application number	不施氮处理 Zero nitrogen （0N）	低氮处理 Low nitrogen （LN）	高氮处理 High nitrogen （HN）
6月June	1	0.00	0.16	0.31
	2	0.00	0.16	0.31
	3	0.00	0.16	0.31
7月July	4	0.00	0.33	0.66
	5	0.00	0.33	0.66
	6	0.00	0.33	0.66
8月August	7	0.00	0.37	0.73
	8	0.00	0.37	0.73
	9	0.00	0.37	0.73
9月September	10	0.00	0.22	0.45
9月September	11	0.00	0.22	0.45

处理 Treatments			微生物生物量碳 Microbial biomass carbon （MBC）	微生物生物量氮 Microbial biomass nitrogen （MBN）	微生物生物量磷 Microbial biomass phosphorus （MBP）
?S	+M	0N	100.79±3.79bcd	16.73±1.09ab	3.49±0.26b
		LN	127.76±9.01abc	6.77±1.47cde	2.93±0.57bcd
		HN	129.19±14.33abc	8.89±1.62c	5.03±0.5a
	?M	0N	142.28±24.25a	14.17±3.66b	1.75±0.25d
		LN	136.7±6.34ab	19.25±0.69a	3.15±0.28bc
		HN	157.94±10.83a	17.28±1.23ab	3.04±0.61bcd
+S	+M	0N	85.03±14.15d	2.19±0.25ef	3.34±0.32b
		LN	90.6±8.69cd	5.27±0.8cdef	3.13±0.46bcd
		HN	166.17±3.16a	7.24±1.29cd	3.46±0.24b
	?M	0N	95.12±12.78cd	1.81±0.02f	2.28±0.41bcd
		LN	73.29±8.28d	3.87±0.84def	1.89±0.49cd
		HN	70.85±14.74d	2.63±0.53def	2.95±0.40bcd

处理 Treatments			β-1,4葡萄糖苷酶 β-1,4 glucosidase (BG)	β-1,4-N-乙酰-氨基葡糖氨糖苷酶和亮氨酸氨基肽酶 β-1,4-N-acetyl-glucosaminidase and leucine aminopeptidase (NAG+LAP)	碱性磷酸酶 Alkaline phosphatase (ALP)
?S	+M	0N	16.48±0.69cd	77.26±3.91b	19.40±1.46cd
		LN	16.38±0.19cd	81.62±2.30ab	22.03±0.15bc
		HN	17.50±0.77bc	88.12±2.64a	27.50±1.67a
	?M	0N	19.79±0.14a	40.63±2.13e	17.96±0.37d
		LN	19.22±1.60ab	54.92±0.99d	23.15±2.24b
		HN	14.57±0.36d	66.62±6.35c	19.04±0.99cd
+S	+M	0N	6.75±0.87e	15.28±1.80f	8.25±0.39e
		LN	6.66±0.20e	12.95±0.70fg	9.64±0.59e
		HN	7.31±0.82e	14.43±2.18f	8.80±1.4e
	?M	0N	2.41±0.29f	5.90±0.59g	7.49±0.14e
		LN	2.58±0.23f	19.16±2.64f	8.36±0.48e
		HN	2.53±0.09f	15.51±0.80f	8.23±0.99e

处理 Treatments			可溶性有机碳 Dissolved organic carbon (DOC)/(mg·g^?1)	有效氮 Available nitrogen (AN)/(mg·kg^?1)	有效磷 Available phosphorus (AP)/(mg·kg^?1)
?S	+M	0N	5.05±0.11a	3.36±0.14de	3.23±0.14b
		LN	5.08±0.52a	3.09±0.69de	3.67±0.18a
		HN	4.57±0.05ab	4.48±0.66cd	2.83±0.21bcd
	?M	0N	3.95±0.6bc	3.74±0.59de	2.65±0.19cde
		LN	3.23±0.07cd	3.65±0.87de	2.52±0.23def
		HN	3.53±0.11cd	4.55±0.93cd	3.07±0.13bc
+S	+M	0N	3.29±0.19cd	2.44±0.05e	2.09±0.06f
		LN	2.86±0.22d	3.75±0.54de	2.09±0.08f
		HN	3.11±0.10cd	3.69±0.04de	2.23±0.17ef
	?M	0N	3.78±0.07bc	5.95±0.33de	2.89±0.11bcd
		LN	3.63±0.22cd	7.38±0.28ab	3.05±0.13bc
		HN	3.64±0.11cd	8.04±1.01a	2.53±0.07def

处理 Treatments			总根长 Root length/cm	总表面积 Root surface area/cm2	总体积 Root volume/cm³	根尖数 Root tips
?S	+M	0N	265.19±6.13b	26.14±0.96b	0.23±0.01b	709.67±47.18d
		LN	252.08±23.66b	23.19±0.94bc	0.21±0.01b	1201±1.15b
		HN	146.21±16.57cd	14.39±1.40de	0.14±0.01c	618.67±84.07d
	?M	0N	34.92±5.11e	1.73±0.29g	0.01±0f	72±16.09h
		LN	121.07±22.52cd	6.25±0.97fg	0.03±0.01ef	269±50.76efg
		HN	78.16±19.05de	6.48±1.03fg	0.04±0.01ef	118±18.19gh
+S	+M	0N	174.56±3.15c	8.99±0.25ef	0.05±0.01ef	401.67±12.57e
		LN	308.02±5.46b	18.40±0.11cd	0.12±0cd	870.67±108.83c
		HN	496.57±67.79a	45.03±6.19a	0.41±0.05a	1782±94.20a
	?M	0N	86.50±10.67de	5.75±0.78fg	0.04±0.01ef	154±4.58gh
		LN	181.40±9.92c	11.80±0.78ef	0.08±0.01de	340.67±17.34ef
		HN	113.76±0.38cd	7.43±0.11fg	0.05±0ef	198.67±13.78fgh

Regulating Effects of Soil Microorganisms and Nitrogen Addition on Rhizosphere Microhabitat and Root Morphology of Caragana korshinskii

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因变量 Dependent variable	参数 Parameter	系数估计值 Estimate	标准化回归系数 Beta	t	P
RL	常量Constant	279.723		2.870	<0.01
	MBC	1.732	0.492	3.016	<0.01
	MBP	63.194	0.506	3.428	<0.01
	EE-GRSP	?1.617	?0.461	?3.284	<0.01
	ALP	?10.736	?0.605	?3.776	<0.01
RS	常量Constant	12.820		1.212	>0.05
	MBC	0.171	0.514	3.523	<0.01
	MBP	6.593	0.558	4.159	<0.01
	AN	?2.181	?0.343	?2.491	<0.05
	AP	9.113	0.388	2.607	<0.05
	EE-GRSP	?0.168	?0.506	?3.995	<0.01
	ALP	?1.312	?0.781	?4.422	<0.01
RV	常量Constant	0.082		0.859	>0.05
	MBC	0.002	0.558	4.018	<0.01
	MBP	0.060	0.537	4.210	<0.01
	AN	?0.020	?0.329	?2.505	<0.05
	AP	0.092	0.413	2.910	<0.01
	EE-GRSP	?0.002	?0.545	?4.519	<0.01
	ALP	?0.012	?0.722	?4.298	<0.01
RT	常量Constant	776.321		1.849	>0.05
	MBC	7.739	0.556	4.009	<0.01
	MBP	257.223	0.521	4.089	<0.01
	AN	?95.210	?0.359	?2.740	<0.05
	AP	297.157	0.303	2.142	<0.05
	EE-GRSP	?7.707	?0.556	?4.622	<0.01
	ALP	?49.256	?0.702	?4.184	<0.01

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