Scientia Silvae Sinicae ›› 2023, Vol. 59 ›› Issue (8): 40-51.doi: 10.11707/j.1001-7488.LYKX20220802
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Xinlu Xu1,2,3,Ou Cai1,2,3,Wanqi Zhao1,2,3,Zhuo Lü1,2,3,Wenjing Yao1,2,Long Li1,2,Shuyan Lin1,2,*()
Received:
2022-11-18
Online:
2023-08-25
Published:
2023-10-16
Contact:
Shuyan Lin
E-mail:lrx@njfu.com.cn
CLC Number:
Xinlu Xu,Ou Cai,Wanqi Zhao,Zhuo Lü,Wenjing Yao,Long Li,Shuyan Lin. Changes in Cytology and Photosynthetic Pigments during the Formation of Colored Leaves of Sasaella glabra ‘Albostriata’[J]. Scientia Silvae Sinicae, 2023, 59(8): 40-51.
Table 1
Description of leaf characteristics at different developmental stages of S. glabra ‘Albostriata’"
时期Stage | 特征描述Description of leaf characteristics |
S1 | 位于靓竹枝条顶部且被叶鞘包裹,未露出叶鞘, 卷曲,嫩白色 Located at the top of branch and wrapped in a leaf sheath, without exposing the leaf sheath, curled, tender white |
S2 | 位于枝条顶部且刚伸出叶鞘0.5 cm以内,卷曲,嫩黄色叶片出现浅绿色条纹 Located at the top of the branch and just protruding within 0.5 cm of the leaf sheath, curled, with light green stripes on tender yellow leaves |
S3 | 位于靓竹枝条顶部,伸出叶鞘,舒展中,具黄绿相间条纹 Located at the top of branch, protruding from the leaf sheath, spreading out with alternating yellow and green stripes |
S4 | 功能叶,叶片完全展开,黄绿相间条纹明显 Functional leaves, fully unfolded, with distinct yellow green stripes |
Fig.2
Microstructure comparison of S. glabra ‘Albostriata’ in different stages a, b, and c are the cell structures of S. glabra ‘Albostriata’ of S1, S2, and S3, respectively; d and e are cell structures of GS and YS in S. glabra ‘Albostriata’ leaf at S4 stage respectively. UE: upper epidermis; LE: lower epidermis; bc: bulliform cell; ac: arm cell; rc: rosette cell; fc: fusoid cell; scale bars: 75 μm in images a and b; 100 μm in images c, d and e."
Table 2
Leaves microstructure parameters in S. glabra ‘Albostriata’"
时期 Stage | 叶片厚度 Leaf thickness/μm | 上表皮厚度 Upper epidermis thickness/μm | 下表皮厚度 Lower epidermis thickness/μm | 泡状细胞 Bulliform cell length/μm | 梅花状细胞 Rosette cell thickness/μm | 指状臂细胞 Arm cell thickness/μm | 梭形细胞 空隙长度 Fusoid cell thickness/μm | |
S1 | 88.40±6.96c | 8.63±1.67c | 8.89±1.51c | 29.55±10.39e | 未分化Undifferentiation | 未分化Undifferentiation | 未分化Undifferentiation | |
S2 | 97.78±5.07b | 9.75±1.66b | 9.47±1.15c | 35.94±11.40d | 未分化Undifferentiation | 未分化Undifferentiation | 未分化undifferentiation | |
S3 | 100.69±6.92b | 9.97±1.63b | 10.41±1.75bc | 55.09±14.29c | 12.50±2.41b | 15.40±2.91a | 58.14±10.86a | |
S4 | 黄区Yellow stripe | 103.44±17.07b | 11.24±1.321a | 13.82±2.53a | 64.03±11.69b | 13.44±2.50a | 16.39±3.64a | 63.43±9.83a |
绿区 Green stripe | 108.25±8.46a | 11.80±1.51a | 14.02±1.83a | 85.05±12.48a | 13.65±2.62a | 16.36±3.10a | 60.74±9.57a |
Table 3
Leaves microstructure parameters in S. glabra ‘Albostriata’"
时期 Stage | 主脉面积 Area of main vascular/μm2 | 1级脉面积 Area of first vascular/μm2 | 2级脉面积 Area of second vascular/μm2 | |
S1 | 3 000.83±484.59d | 1 639.58±435.65e | 453.12±109.22c | |
S2 | 3 776.53±506.92c | 2 711.88±519.29d | 555.10±132.80b | |
S3 | 5 152.21±452.31b | 3 877.31±940.31c | 617.41±133.23b | |
S4 | 黄区 Yellow stripe | 9 076.42±1425.09a | 4 776.94±493.83b | 775.68±279.24a |
绿区 Gre en stripe | 9 155.99±1260.08a | 5 298.49±661.75a | 806.14±198.89a |
Fig.5
Ultrastructure of epidermis and transsections of S. glabra ‘Albostriata’ in different stripes a–f is the upper epidermis, lower epidermis, leaf transverse section, papilla, stomata and mesophyll cells of the green district of bamboo leaves; g–l is respectively the upper epidermis, lower epidermis, leaf transverse section, papilla, stomata and mesophyll cells of the yellow area of the bamboo leaves."
Table 4
Changes of leaves photosynthetic pigment content during different stages of S. glabra ‘Albostriata’"
时期Stage | 叶绿素a Chlorophyll a/( mg·g?1) | 叶绿素b Chlorophyll b/( mg·g?1) | 叶绿素 Chlorophyll/( mg·g?1) | 类胡萝卜素 Carotenoids/( mg·g?1) |
S1 | 0.297±0.037c | 0.074±0.032b | 0.370±0.037c | 0.236±0.016c |
S2 | 0.364±0.213c | 0.198±0.090b | 0.562±0.228c | 0.327±0.042c |
S3 | 9.441±0.767b | 2.169±0.496a | 11.610±1.23b | 3.206±0.188b |
S4 | 11.941±2.497a | 2.202±0.471a | 14.143±2.961a | 4.282±0.825a |
Table 5
Photosynthetic pigment content comparision in different stripes at S4 stage of S. glabra ‘Albostriata’"
表型Phenotype | 叶绿素a Chlorophyll a/( mg·g?1) | 叶绿素b Chlorophyll b/( mg·g?1) | 叶绿素 Chlorophyll/( mg·g?1) | 类胡萝卜素 Carotenoids/( mg·g?1) | 叶绿素/类胡萝卜素 Chlorophyll/Carotenoids |
S4 | 14.186±2.608c | 3.623±1.202b | 17.809±3.806c | 6.247±0.731b | 3.098±0.141a |
YS | 2.169±0.431d | 1.250±0.617c | 3.419±1.045d | 1.158±0.174c | 2.952±0.399c |
GS | 24.977±2.681b | 5.407±0.971b | 30.384±3.648b | 8.943±0.664a | 3.542±0.153b |
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