美国白蛾对摩西球囊霉定殖银中杨的适应和生理响应

doi:10.11707/j.1001-7488.20220609

Abstract

Abstract:

Objective: This study aims to investigate the adaptation and physiological response of Hyphantria cunea (Lepidoptera: Arctiidae) to Populus alba × P. berolinensis colonized with arbuscular mycorrhizae fungi (AMF). Method: The cuttings of P. alba × P. berolinensis were inoculated with Glomus mosseae (GM), and when the GM infection rate reached the highest (90 days), the leaves were harvested and fed to the larvae of H. cunea. Result: After feeding on GM infected leaves of the popular, the body weight of 3^rd-6^th instar larvae was lower than that of the control, but the difference was not significant. The development duration of 2^nd-5^th instar larvae was significantly prolonged, and the relative growth rates of 3^rd and 5^th instar larvae were significantly decreased. There was no significant difference in food digestion rate of 5^th instar larvae of H. cunea, but food conversion rate and utilization rate were inhibited. There was no significant difference in food consumption rate, conversion rate and utilization rate of 6^th instar larvae. The activity of lipase in 5^th instar larvae was significantly increased, the activity of glutathione S-transferase (GSTs) was inhibited, and the activities of protease, amylase and carboxylesterase (CarE) were not significantly different from those in the control group. The activities of lipase, protease, amylase, GSTs and CarE of 6^th instar larvae were significantly higher than those of the control. Conclusion: The P. alba × P. berolinensis inoculated with GM has a certain inhibitory effect on the growth, development and food utilization of H. cunea in a short period of time, and H. cunea can break through this inhibition by increasing the activities of digestive enzymes and detoxification enzymes, and maintain normal growth and development. The results can lay a theoretical foundation for the future study of AMF-forest-herbivorous insect system.

Key words: Hyphantria cunea, arbuscular mycorrhizae fungi (AMF), Glomus mosseae, growth and development, food utilization, digestive enzyme, detoxification enzyme

CLC Number:

S718.7

Mingtao Tan,Dun Jiang,Shuai Wu,Jie Zhang,Lei Liu,Jiaqi Zhao,Zhaojun Meng,Shanchun Yan. Adaptation and Physiological Response of Hyphantria cunea (Lepidoptera: Arctiidae) to Populus alba × P. berolinensis Colonized by Glomus mosseae[J]. Scientia Silvae Sinicae, 2022, 58(6): 88-94.

Figures/Tables 6

Table 1

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

References 0

	陈澄宇, 康志娇, 史雪岩, 等. 昆虫对植物次生物质的代谢适应机制及其对昆虫抗药性的意义. 昆虫学报, 2015, 58 (10): 1126- 1139.
	Chen C Y , Kang Z J , Shi X Y , et al. Metabolic adaptation mechanisms of insects to plant secondary metabolites and their implications for insecticide resistance of insects. Acta Entomologica Sinica, 2015, 58 (10): 1126- 1139.
	陈锐, 高贺, 张国军, 等. 小麦籽粒次生物质对麦红吸浆虫幼虫解毒酶活性及基因表达的影响. 中国农业科学, 2020, 53 (20): 4204- 4214. doi: 10.3864/j.issn.0578-1752.2020.20.009
	Chen R , Gao H , Zhang G J , et al. Effects of secondary metabolites in wheat kernels on activities of three detoxifying enzymes and related gene expression in Sitodiplosis mosellena. Scientia Agricultura Sinica, 2020, 53 (20): 4204- 4214. doi: 10.3864/j.issn.0578-1752.2020.20.009
	董艳, 董坤, 杨智仙, 等. AM真菌控制蚕豆枯萎病发生的根际微生物效应. 应用生态学报, 2016, 27 (12): 4029- 4038.
	Dong Y , Dong K , Yang Z X , et al. Rhizosphere microbial impacts of alleviating faba bean Fusarium wilt with inoculating AM fungi. Chinese Journal of Applied Ecology, 2016, 27 (12): 4029- 4038.
	樊艳平, 党海燕, 王宏民, 等. 绿豆胰蛋白酶抑制剂对绿豆象生长发育及体内解毒酶和保护酶活性的影响. 昆虫学报, 2020, 63 (12): 1473- 1481.
	Fan Y P , Dang H Y , Wang H M , et al. Effects of mung bean trypsin inhibitor on the growth and development and the activities of detoxifying and protective enzymes in Callosobruchus chinensis (Coleoptera: Bruchidae). Acta Entomologica Sinica, 2020, 63 (12): 1473- 1481.
	辜晓婷, 覃金转, 王秀荣. 接种菌根真菌对不同磷效率基因型大豆生长和磷吸收的影响. 中国生态农业学报(中英文), 2020, 28 (3): 357- 364.
	Gu X T , Qin J Z , Wang X R , et al. Effect of mycorrhizal fungal inoculation on the growth and phosphorus uptake by soybean genotypes with different phosphorus use efficiency. Chinese Journal of Eco-Agriculture, 2020, 28 (3): 357- 364.
	何磊, 邹慧芳, 李长友, 等. 丛枝菌根真菌和甜菜夜蛾的相互作用. 植物保护学报, 2017, 44 (3): 460- 466.
	He L , Zou H F , Li C Y , et al. Interactions between arbuscular mycorrhizal fungi and beet armyworm Spodoptera exigua (Hübner)(Lepidoptera: Noctuidae). Journal of Plant Protection, 2017, 44 (3): 460- 466.
	黄敏燕, 李雪峰. 植物次生物质对斜纹夜蛾解毒酶活性的影响. 基因组学与应用生物学, 2018, 37 (8): 3495- 3502.
	Huang M Y , Li X F . Effects of plant secondary metabolite on detoxification enzyme activity of Spodoptera litura. Genomics and Applied Biology, 2018, 37 (8): 3495- 3502.
	姜礅. 2019. 重金属胁迫下银中杨抗虫性及食叶害虫舞毒蛾解毒机制研究. 哈尔滨: 东北林业大学.
	Jiang D. 2019. Study on the insect resistance of Populus alba × P. berolinensis and the detoxification mechanism of defoliator, Lymantria dispar under heavy metal stress. Harbin: Northeast Forestry University. [in Chinese]
	姜礅, 孟昭军, 严善春. 用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响. 北京林业大学学报, 2017, 39 (2): 58- 63.
	Jiang D , Meng Z J , Yan S C . Effects of partially spraying Larix olgensis seedlings with exogenous methyl jasmonate on the defensive enzyme activities of Dendrolimus superans larvae. Journal of Beijing Forestry University, 2017, 39 (2): 58- 63.
	李寿银, 陈聪, 李慧, 等. 取食含不同植物源成分的饲料对香樟齿喙象幼虫生长发育及体内解毒酶活性的影响. 昆虫学报, 2019, 62 (11): 1286- 1296.
	Li S Y , Chen C , Li H , et al. Effects of feeding on diets containing components of different plants on the development and detoxifying enzyme activities in Pagiophloeus tsushimanus (Coleptera: Curculionidae) larvae. Acta Entomologica Sinica, 2019, 62 (11): 1286- 1296.
	李艺琼, 姚羽芯, 彭正强, 等. 不同生长阶段椰树叶片对椰心叶甲中肠消化酶活性的影响. 江苏农业科学, 2017, 45 (7): 94- 97.
	Li Y Q , Yao Y X , Peng Z Q , et al. The effect of palm tree leaves on midgut digestive enzyme activity of Brontispa longissima with different growth phases. Jiangsu Agricultural Sciences, 2017, 45 (7): 94- 97.
	鲁智慧, 和淑琪, 郭晋, 等. 取食不同寄主植物对草地贪夜蛾幼虫体内3类酶活性的影响. 南方农业学报, 2020, 51 (10): 2461- 2469.
	Lu Z H , He S Q , Guo J , et al. Effects of host plants on activities of three groups of enzymes in Spodoptera frugiperda (Smith) larvae. Journal of Southern Agriculture, 2020, 51 (10): 2461- 2469.
	马孝龙. 2019. 氰氟虫腙对斜纹夜蛾的亚致死效应. 雅安: 四川农业大学.
	Ma X L. 2019. Sublethal effect of metaflumizone on Spodoptera exigua. Ya'an: Sichuan Agricultural University. [in Chinese]
	潘忠玉, 莫夏娜, 孟香, 等. 绿原酸对美国白蛾幼虫生长发育和解毒相关蛋白活性的影响. 昆虫学报, 2020, 63 (9): 1081- 1090.
	Pan Z Y , Mo X N , Meng X , et al. Effects of chlorogenic acid on the growth and development and detoxification-related protein activities in Hyphantria cunea (Lepidoptera: Arctiidae) larvae. Acta Entomologica Sinica, 2020, 63 (9): 1081- 1090.
	秦海滨, 张志斌, 贺超兴. 丛枝菌根真菌提高黄瓜幼苗抗立枯病作用研究. 华北农学报, 2014, 29 (S1): 98- 102.
	Qin H B , Zhang Z B , He C X . Study of tolerance effects on blight of cucumber seedlings induced by arbuscular mycorrhizal fungi. Acta Agriculturae Boreali-Sinica, 2014, 29 (S1): 98- 102.
	向玉勇, 孙星, 殷培峰. 寄主植物、温度对金银花尺蠖幼虫消化酶活性的影响. 浙江农林大学学报, 2020, 37 (2): 311- 318.
	Xiang Y Y , Sun X , Yin P F . Effects of host plants and temperatures on digestive enzyme activities in Heterolocha jinyinhuaphaga larvae. Journal of Zhejiang A and F University, 2020, 37 (2): 311- 318.
	邢易梅, 蕫理, 战力峰, 等. 混合接种摩西球囊霉和根瘤菌对紫花苜蓿耐碱能力的影响. 草业学报, 2020, 29 (9): 136- 145.
	Xing Y M , Dong L , Zhan L F , et al. Effect of mixed inoculation of Glomus mosseae and Sinorhizobium melilotion alkali resistance of alfalfa. Acta Prataculturae Sinica, 2020, 29 (9): 136- 145.
	薛世通, 董琦, 董泽鹏, 等. 施氮量对春小麦/豌豆间作生长、AMF侵染率和春小麦产量的影响. 河南农业科学, 2020, 49 (4): 22- 28.
	Xue S T , Dong Q , Dong Z P , et al. Effects of nitrogen application rates on spring wheat and pea growth, AMF infection rate and spring wheat intercropping with pea. Journal of Henan Agricultural Sciences, 2020, 49 (4): 22- 28.
	杨国日, 莫继申. 银中杨硬枝扦插与大苗繁育技术. 辽宁林业科技, 2020, (4): 70- 71.
	Yang G R , Mo J S . Hard wood cutting and big-seedling breeding techniques of Populus alba × P. berolinensis. Liaoning Forestry Science and Technology, 2020, (4): 70- 71.
	张蓓蓓, 张辉, 景琦, 等. 两种水分条件下真菌接种及氮肥施加对小麦生长、生理及氮磷吸收的影响. 干旱地区农业研究, 2019, 37 (1): 214- 220.
	Zhang B B , Zhang H , Jing Q , et al. Effect of mycorrhizal fungi inoculation and nitrogen fertilization on physiological characteristics, growth, and nitrogen and phosphorus uptake of wheat under two distinct water regimes. Agricultural Research in the Arid Areas, 2019, 37 (1): 214- 220.
	Baghban A , Sendi J J , Zibaee A , et al. Effect of heavy metals (Cd, Cu, and Zn) on feeding indices and energy reserves of the cotton boll worm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Journal of Plant Protection Research, 2014, 54 (4): 367- 373.
	Bai P H , Wang H M , Liu B S , et al. Botanical volatiles selection in mediating electrophysiological responses and reproductive behaviors for the fall webworm moth Hyphantria cunea. Frontiers in Physiology, 2020, 11, 486.
	Benhiba L , Fouad M O , Essahibi A , et al. Arbuscular mycorrhizal symbiosis enhanced growth and antioxidant metabolism in date palm subjected to long-term drought. Trees, 2015, 29 (6): 1725- 1733.
	Chen Q , Zhao H B , Wen M , et al. Genome of the webworm Hyphantria cunea unveils genetic adaptations supporting its rapid invasion and spread. BMC Genomics, 2020, 21 (1): 242.
	Gao X P , Guo H H , Zhang Q , et al. Arbuscular mycorrhizal fungi(AMF)enhanced the growth, yield, fiber quality and phosphorus regulation in upland cotton(Gossypium hirsutum L). Scientific Reports, 2020, 10 (1): 2084.
	Jung S C , Martinez-Medina A , Lopez-Raez J A , et al. Mycorrhiza-induced resistance and priming of plant defenses. Journal of Chemistry Ecology, 2012, 38 (6): 651- 664.
	Murrell E G , Ray S , Lemmon M E , et al. Cover crop species affect mycorrhizae-mediated nutrient uptake and pest resistance in maize. Renewable Agriculture and Food Systems, 2020, 35 (5): 467- 474.
	Nishida T , Katayama N , Izumi N , et al. Arbuscular mycorrhizal fungi species-specifically affect induced plant responses to a spider mite. Population Ecology, 2010, 52 (4): 507- 515.
	Phillips J M , Hayman D S . Improved procedures for clearing roots and staining parasitic and vesicular arbuscular fungi for rapid assessment of infection. Transactions of the British Mycological Society, 1970, 55, 158- 161.
	Selvaraj A , Thangavel K , Uthandi S , et al. Arbuscular mycorrhizal fungi (Glomus intraradices) and diazotrophic bacterium (Rhizobium BMBS) primed defense in blackgram against herbivorous insect (Spodoptera litura) infestation. Microbiological Research, 2020, 231, 126355.
	Shrivastava G , Ownley B H , Augé R M , et al. Colonization by arbuscular mycorrhizal and endophytic fungi enhanced terpene production in tomato plants and their defense against a herbivorous insect. Symbiosis, 2015, 65 (2): 65- 74.
	Tao L , Ahmad A , de Roode J C , et al. Arbuscular mycorrhizal fungi affect plant tolerance and chemical defences to herbivory through different mechanism. Journal of Ecology, 2016, 104 (2): 561- 571.
	Wu N , Zhang S F , Li X , et al. Fall webworm genomes yield insights into rapid adaptation of invasive species. Nature Ecology & Evolution, 2019, 3 (1): 105- 115.
	Yazdanfar H , Ghodskhah D M , Jalali S J . The effects of host plants on the feeding indices and chemical activities of elm leaf beetle, Xanthogaleruca luteola (Muller) (Coleoptera: Chrysomelidae). Iran Agricultural Research, 2016, 35 (1): 81- 87.
	Zhang L W , Kang K , Liu Y J , et al. Evaluation of Beauveria bassianaisolates as potential agents forcontrol of Hyphantria cunea (Lepidoptera: Arctiidae). Acta Entomologica Sinica, 2016, 59, 111- 118.
	Zhang M , Fang T , Pu G , et al. Xenobiotic metabolism of plant secondary compounds in the English grain aphid, Sitobion avenae(F.) (Hemiptera: Aphididae). Pesticide Biochemistry and Physiology, 2013, 107 (1): 44- 49.

处理	平均体质量Average body weight of larvae/g
处理	3龄 3rd instar	4龄 4th instar	5龄 5th instar	6龄 6th instar
CK	0.011 9±0.001 6	0.020 5±0.002 8	0.042 7±0.008 3	0.104 3±0.029 9
GM	0.011 0±0.001 8	0.018 5±0.003 1	0.042 3±0.006 9	0.100 2±0.022 2

[1]	Jiangxia Ye,Jingwen Wang,Mingsha Zhang,Ruliang Zhou,Lei Shi. Risk Pattern Analysis of Hyphantria cunea Based on Spatial Matrix Model and 0-1 Measure [J]. Scientia Silvae Sinicae, 2021, 57(1): 140-152.
[2]	Shaobo Wang,Zhou Zhou,Yimeng Chen,Yuzhu Wang,Yong Zhang,Liangjian Qu. Diapause of Hyphantria cunea (Lepidoptera: Arctiidae) Induced by Photoperiod and Temperature [J]. Scientia Silvae Sinicae, 2020, 56(4): 121-127.
[3]	Yue Wang,Sufang Zhang,Yao Xu,Fu Liu,Xiangbo Kong,Zhen Zhang. Cloning and Expression Profiles of HcSID-1 Gene and Its Function Verification in Hyphantria cunea Larvae [J]. Scientia Silvae Sinicae, 2019, 55(10): 48-56.
[4]	Yuan Ran, Li Xinyue, Wu Shaoping, Cao Chuanwang. Effects of Elevated CO₂ Concentration on Growth and Development of Lymantria dispar (Lepidoptera: Lymantridae), as well as on Activities of Detoxifying Enzymes and Protective Enzymes in the Body [J]. Scientia Silvae Sinicae, 2018, 54(12): 102-109.
[5]	Zeng Jianyong, Zhang Fangming, Wu Yue, Zhang Tingting, Zhang Guocai. Synergy Mechanism of Abamectin and Triflumuron on Lymantria dispar Larvae [J]. Scientia Silvae Sinicae, 2018, 54(12): 110-115.
[6]	Sun Lizhu, Luo Lan, Yuan Zhonglin. Effects of Extracts of Lantana camara (Verbenaceae) Leaves on Cellulose and Detoxifying Enzymes Activities of Reticulitermes flaviceps (Isoptera: Rhinotermitidae) [J]. Scientia Silvae Sinicae, 2017, 53(5): 107-115.
[7]	Liu Huihui, Zhang Yong, Wang Yuzhu, Zeng Baosheng, Liu Qun, Zhang Zhen. Genome Editing of Wnt-1 in Fall Webworm (Hyphantria cunea) [J]. Scientia Silvae Sinicae, 2017, 53(3): 119-127.
[8]	Gao Yue, Qiu Cailou, Wang Guangbiao, Xie Chunxia, Cheng Cong. Flight Parameters of Unmanned Aerial Vehicle(UAV) in Spraying the Insecticide against Hyphantria cunea and the Control Effects [J]. Scientia Silvae Sinicae, 2017, 53(12): 147-152.
[9]	Su Xiaoyu, Wang Jing, Ren Xiaojing, Li Huiping. Ultraviolet Mutation Breeding for High Toxicity Strains of Beauveria bassiana against Larvae of Hyphantria cunea (Drury) [J]. Scientia Silvae Sinicae, 2016, 52(7): 165-169.
[10]	Xu Ming, Xu Fuyuan, Wu Xiaoqin. Effects of the Mixture of Bt and Chlorbenzuron on the Activity of Midgut Proteinase, Haemolymph Proteins on 4^th Instar Larvae of Hyphantria cunea [J]. Scientia Silvae Sinicae, 2015, 51(11): 137-144.
[11]	Zhang Xiongshuai, Zhou Guona, Gao Baojia. Response Mechanisms of Pine Caterpillar Enzymatic System to Pine Induced Resistance [J]. Scientia Silvae Sinicae, 2014, 50(10): 181-187.
[12]	Zhang Yan;Guo Tongbin;Zhuge Qiang. Effects of Bt Nanlin 895 Poplar on the Metabolic Enzymes of Scarce Chocolate Tip, Clostera anachoreta [J]. Scientia Silvae Sinicae, 2012, 48(6): 95-99.
[13]	Liu Dan;Yan Shanchun;Cao Chuanwang;Liao Yuezhi. Effect of Spinosad on the Detoxifying and Protective Enzymes of Malacosoma neustria testacea [J]. Scientia Silvae Sinicae, 2012, 48(4): 67-74.
[14]	Guo Tongbin;Ji Baozhong;Zhuge Qiang;Huang Minren. Effect of Transgenic Poplars on the Activities of Detoxification Enzymes in Micromelalopha troglodyta Larvae [J]. Scientia Silvae Sinicae, 2007, 43(5): 59-63.
[15]	Cao Haiqun;Yue Yongde;Peng Zhenhua;Hua Rimao;Tang Feng. Effects of Bamboo Extracts on the Activities of Several Enzymes in Helicoverpa armigera Larvae [J]. Scientia Silvae Sinicae, 2006, 42(7): 145-148.

Adaptation and Physiological Response of Hyphantria cunea (Lepidoptera: Arctiidae) to Populus alba × P. berolinensis Colonized by Glomus mosseae

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 0

Related Articles 15

Recommended Articles

Metrics

Comments