杉木林土壤和苗木内生溶磷细菌的筛选及其溶磷特性

doi:10.11707/j.1001-7488.20201201

Abstract

Abstract:

Objective: This study aimed to screen and identify efficient phosphate solubilizing bacteria from Chinese fir, We investigated its phosphate solubilizing characteristics, and explored ecological adaptability, so as to provide bacterial resources and culture conditions for the development of microbial fertilizer for Chinese fir. Method: Phosphate solubilizing bacteria were isolated from soil and Chinese fir plant by PVK inorganic phosphorus medium, and identified by physiological and biochemical tests as well as 16S rDNA gene sequence. A single factor test was used to analyze the phosphate solubilizing characteristics of phosphate solubilizing bacteria under different pH, temperature, carbon source, nitrogen source and phosphorus source. Result: 1) A total of 20 strains of phosphate solubilizing bacteria were isolated and screened, and the three strains with the strongest phosphate solubilizing capacity were P5, RP2 and RP22 (195.61, 109.20 and 78.86 mg·L^-1, respectively). 2) Strain P5 was identified as Burkholderia ubonensis, RP2 as Pseudomonas frederiksbergensis and RP22 as Pseudomonas grimontii by physiological and biochemical tests, combined with 16S rDNA sequence analysis and phylogenetic tree construction. 3) The results of single factor experiment showed that the P5 strain had the strongest phosphate solubilizing ability when the pH was 5, the temperature was 30℃, the carbon source was sucrose, the nitrogen source was ammonium chloride, and the phosphorus source was calcium phosphate. RP2 showed the strongest phosphate solubilizing ability when pH7, 30℃, the carbon source was glucose, the nitrogen source was potassium nitrate, and the phosphorus source was calcium phosphate. RP22 showed the strongest phosphate solubilizing ability when the pH7, 25℃, the carbon source was glucose, the nitrogen source was potassium nitrate, and the phosphorus source was calcium phosphate. Conclusion: The screened bacteria have high phosphate solubilizing ability among which strain P5 has an efficient phosphate solubilizing ability under acidic and high temperature conditions, which has great potential for the development of microbial fertilizers.

Key words: Chinese fir, phosphate solubilizing bacteria, screening, identification, culture conditions

CLC Number:

S714.3

Yihui Wei,Jiaqi Chen,Guangyu Zhao,Yuhong Dong,Lingyu Hou,Ruzhen Jiao. Screening of Phosphate Solubilizing Bacteria from Soil and Endogenous Environment of Chinese Fir Seedlings and Their Characterization of Phosphate Solubilization[J]. Scientia Silvae Sinicae, 2020, 56(12): 1-9.

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菌株 Strain No.	分离部位 Isolated part	形状 Shape	颜色 Color	表面状态 Surface state	菌株 Strain No.	分离部位 Isolated part	形状 Shape	颜色 Color	表面状态 Surface state
P1	S	C	T	M	P11	S	C	W	M
P2	S	C	W	M	P12	S	C	W	M
P3	S	C	T	M	P13	S	C	T	M
P4	S	C	T	M	SP13	St	C	LY	M
P5	S	C	W	M	LP2	L	C	W	D
P6	S	C	T	M	RP2	R	C	W	D
P7	S	C	W	D	RP13	R	C	W	M
P8	S	C	W	M	RP22	R	C	LY	M
P9	S	C	W	M	RP23	R	C	PY	SM
P10	S	C	PY	M	RP24	R	C	W	M

菌株 Strain No.	可溶性磷含量 Soluble phosphorus content	菌株 Strain No.	可溶性磷含量 Soluble phosphorus content
P1	21.61±1.82h	P11	50.77±2.45e
P2	52.68±4.86e	P12	47.08±1.29f
P3	45.67±3.94f	P13	51.73±1.97e
P4	39.62±3.59f	SP13	65.17±5.52d
P5	195.61±14.9a	LP2	44.29±2.09f
P6	56.63±4.29e	RP2	109.20±3.06b
P7	49.54±1.10e	RP13	68.22±5.26d
P8	46.54±3.00f	RP22	78.86±5.02c
P9	36.63±2.85g	RP23	74.54±6.68c
P10	24.13±1.81h	RP24	67.83±4.97d

菌株 Strain No.	革兰氏染色 Gram staining	葡萄糖水解 Glucose hydrolysis	乳糖水解 Lactose hydrolysis	甲基红 Methyl red	V-P试验 V-P test	硫化氢试验 Hydrogen sulfide production	明胶液化 Gelatin liquefaction	柠檬酸盐 Citrate	丙二酸盐 Malonate	反硝化 Denitrification
P1	-	+	+	+	-	-	+	+	-	-
P2	-	+	+	-	-	-	-	-	-	+
P3	-	+	+	+	-	-	+	+	-	+
P4	-	+	+	+	-	-	+	+	-	+
P5	-	+	+	-	-	-	-	+	-	+
P6	-	+	+	+	-	-	+	+	-	+
P7	+	+	+	+	-	-	+	+	-	-
P8	-	+	+	+	-	-	+	+	-	+
P9	-	+	+	+	-	-	+	+	-	+
P10	-	+	+	+	-	-	+	+	-	-
P11	-	+	+	+	-	-	+	+	-	-
P12	-	+	+	+	-	-	+	+	-	+
P13	-	+	+	+	-	-	+	+	-	+
SP13	-	+	+	+	-	-	+	+	-	-
LP2	-	+	+	-	+	-	+	+	-	-
RP2	-	+	+	-	+	-	+	+	-	+
RP13	-	+	+	+	-	-	+	+	-	+
RP22	-	+	+	-	+	-	+	+	+	+
RP23	-	+	-	-	-	+	-	-	-	-
RP24	-	+	-	+	-	-	-	-	-	-

菌株 Strain No.	模式菌株 Type strain	相似性 Identity(%)
P1	副伯克氏菌 Paraburkholderia hiiakae (JF763857^T)	98.78
P2	伯克氏菌 Burkholderia humptydooensis (MSMB43^T)	96.52
P3	副伯克氏菌 Paraburkholderia terrae (NBRC00964^T)	98.64
P4	伯克氏菌 Burkholderia arboris (AM47630^T)	99.92
P5	伯克氏菌 Burkholderia ubonensis (CIP107078^T)	98.44
P6	欧文氏菌 Erwinia rhapontici (ATCC29283^T)	99.11
P7	芽孢杆菌 Bacillus aryabhattai (B8W22^T)	100
P8	副伯克氏菌 Paraburkholderia metrosideri (DFBP6-1^T)	97.7
P9	伯克氏菌 Burkholderia oklahomensis (C6786^T)	98.67
P10	泛菌属 Pantoea conspicua (LMG24534^T)	99.63
P11	欧文氏菌 Erwinia billingiae (CIP106121^T)	98.81
P12	副伯克氏菌 Paraburkholderia insulsa (KF733462^T)	99.25
P13	副伯克氏菌 Paraburkholderia metrosideri (DFBP6-1^T)	98.62
SP13	泛菌属 Pantoea roadsii (LMG26273^T)	98.84
LP2	假单胞菌 Pseudomonas donghuensis (HYS^T)	98.75
RP2	假单胞菌 Pseudomonas frederiksbergensis (JAJ28^T)	99.61
RP13	副伯克氏菌 Paraburkholderia caffeinilytica (CF1^T)	99.31
RP22	假单胞菌 Pseudomonas grimontii (CFML97-514^T)	100
RP23	新鞘氨醇菌 Novosphingobium barchaimii (LL02^T)	99.69
RP24	苍白杆菌 Ochrobactrum pseudogrignonense (CCUG30717^T)	100

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Screening of Phosphate Solubilizing Bacteria from Soil and Endogenous Environment of Chinese Fir Seedlings and Their Characterization of Phosphate Solubilization

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