Abstract:

Both morphological characters and a portion of of 28S rDNA sequences were used to identifying Tomicus species. The specimens were classed into groups with the following characters:1) granules or punctures on interstria 2 on the declivity of the elytra. 2) length of the elytral interstrial hairs and hairs arising from punctures. 3)arrangement of pronotal punctures and hairs. These characters could be clearly examined under a binocular microscope at 30×magnification and they were applicable and valuable for the forest entomologists to identify Tomicus species. The phylogenetic tree established with difference in 28S rDNA sequence of D2 region revealed that the specimens of each group identified by morphological characters were also grouped together. The genetic distances of intra-species, inter-species and inter-genus were not overlapped. Genetic divergence of 28S rDNA was also useful for identifying Tomicus species.

Key words: Tomicus, morphology, key to species of Tomicus, 28S rDNA