泡桐的microRNAs及其功能预测

doi:10.11707/j.1001-7488.20131110

Abstract

Abstract:

A sRNA library of the mixed leaves of different Paulownia species and varieties was constructed with the second generation high-throughput sequencing technology and bioinformatics and the functions of microRNA (miRNAs) were analyzed in this paper. The results showed that the 41 399 208 out of the obtained 87 066 707 high-quality reads were perfectly aligned to transcription of Paulownia plant in the small RNAs library, accounting for 47.55%. The length of sRNAs ranged mainly from 20 to 25 nt, and the sRNA of 24 nt was the most abundant, followed by 21 nt reads. The 44 conserved miRNAs, belonging to 14 miRNA families, were identified, and there was difference in miRNA numbers of various miRNAs families. Moreover, functions of the target genes of 27 novel miRNAs were analyzed. These results provided a basis for clarifying miRNA functions during growth and development of Paulownia plants.

Key words: Paulownia, microRNA, high throughput sequencing, functional analysis

CLC Number:

S718.46

Niu Suyan, Fan Guoqiang, Zhao Zhenli, Deng Minjie, Dong Yanpeng. MicroRNAs of Paulownia Plants and Their Functional Prediction[J]. Scientia Silvae Sinicae, 2013, 49(11): 77-82.

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MicroRNAs of Paulownia Plants and Their Functional Prediction

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