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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (7): 57-61.doi: 10.11707/j.1001-7488.20130708

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H2O2 Mediated Synthesis of Betulin in Cells of Betula platyphylla Induced by Fungal Elicitor

Su Meiling, Li Xiaocan, Wang Xiaodong, Zhan Yaguang, Fan Guizhi   

  1. College of Life Science, Northeast Forestry University Harbin 150040
  • Received:2012-06-18 Revised:2013-03-29 Online:2013-07-25 Published:2013-07-23

Abstract:

The role of H2O2 in the synthesis of betulin in cells of birch (Betula platyphylla) induced by fungal elicitor was studied using suspension cell lines as the test materials. The results showed that the exogenously applied H2O2 decreased the cell viability and dry weight accumulation, however promoted the synthesis of betulin. Of them, the content of betulin was increased by 89.45% under treatment with 1 mmol·L-1 H2O2 for 12 h, and was increased by 73.72% under treatment with 0.1 mM H2O2 for 24 h. The fungal elicitor increased the synthesis of H2O2 and betulin by 391.67% and 185.22%, respectively. H2O2 scavenger catalase (CAT) significantly decreased induction effects of the fungal elicitor in H2O2 and betulin production in the birch cells and the contents reduced by 73.10% and 35.96%. The results suggested that H2O2 participated in the fungal elicitor-induced synthesis of betulin in birch cells.

Key words: Betula platyphylla, betulin, fungal elicitor, H2O2, Phomopsis

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