应用nanoLC-MS/MS分析毛白杨次生维管系统的蛋白质表达谱

doi:10.11707/j.1001-7488.20130507

›› 2013, Vol. 49 ›› Issue (5): 43-53.doi: 10.11707/j.1001-7488.20130507

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Proteome Profile in Secondary Vascular System of Populus tomentosa by nanoLC-MS/MS

Jiang Shulei^1,2, Chen Jiafei^1,3, Zhao Shutang^1,2, Lu Mengzhu^1,2

1. State Key Laboratory of Tree Genetics and Breeding Beijing 100091;2. Research Institute of Forestry,Chinese Academy of Forestry Beijing 100091;3. Research Institute of Forestry New Technology,Chinese Academy of Forestry Beijing 100091

Received:2012-09-04 Revised:2013-02-12 Online:2013-05-25 Published:2013-05-25

Abstract

Abstract: As the uppermost carbon pool and important renewable source, wood has dual functions in both responding to the global climate change and meeting the resource demands of human society, thereby it is essential to understand the molecular mechanism of wood formation. In this study, a 1DE-based nanoLC-MS/MS method was introduced to analyze the proteome in the secondary vascular system of Populus tomentosa, and the identified proteins included:14 proteins associated cell division; 7 proteins related to transcription factors; 22 proteins associated with cytoskeleton; 31 proteins associated with signal transduction;and 14 proteins associated with cell wall formation. The corresponding genes of these proteins participate in regulating cambial activity. The accomplishment of this proteome analysis laid a foundation for further understanding the molecular mechanism of wood formation.

Key words: LC-MS/MS, proteome profile, secondary vascular system, Populus tomentosa

CLC Number:

S718.46

Jiang Shulei;Chen Jiafei;Zhao Shutang;Lu Mengzhu;. Proteome Profile in Secondary Vascular System of Populus tomentosa by nanoLC-MS/MS[J]. , 2013, 49(5): 43-53.

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Proteome Profile in Secondary Vascular System of Populus tomentosa by nanoLC-MS/MS

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